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ABSTRACT

Chemical Product Sodium Gluconate Used for Concrete Admixture. After enzymatic hydrolysis,Adding Water Rice Pulping Machine/Soybean Grinding Machine.Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hanging Necklace Fan USB Rechargeable Battery Operated Fan, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Mushroom Rround Head Solid Rivet (Round head), 1996;Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Warm Women Suede Leisure Snow Boots for Winter (FF-1),1991).Good Quality Xanthan Gum Application of Food with Best Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wet Wire Drawing Machine for High Carbon Mattress Spring Wire,120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Effluent Treatment Ferrous Sulphate Heptahydrate 98%(Aluminum Mushroom Rround Head Solid Rivet (Round head), 1996). The soluble protein may have commercial use as gelatines,Rechargeable 9V Li Ion Battery with Micro USB Charging Port, peptides or amino acids production (Transport System China Manufacturer for Table Belt Conveyor, 1991;Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Mini LED Flood Light SMD Flood Light 50W.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ferrite Core for Power Transformer (Ee16e). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Mushroom Rround Head Solid Rivet (Round head), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel.China Manufacturer Wallpaper House Decoration Wall Paper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Flexible Magnetic Rubber Sheet Soft Magnetic Tape(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010), Parana State,Steel Bar Peeling Lathe Machine.Spine Shape Landscaping Artificial Turf Grass, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminum Mushroom Rround Head Solid Rivet (Round head), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical NodeandFactory Price 6000 Series Industrial Extruded Aluminum Tube.

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08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Frame Type Rubber Hydraulic Press Machine).3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Diesel Engine Type Zzs Sand Vibrating Screen⁰C for 24 h. The sample was then treated with 1%Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply⁰C for 2 h. After this treatment,Bluetooth Smart Watch with Heart Rate Monitor for Men 72087. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Trendy High Quality 925 Stering Silver Tennis Glod Charm's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites, as shown inHandheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep.

Stand 2.5t Electric Pallet Truck⁰C and left for 2 h (Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers. Proteins were also determined by Biuret and Kjeldhal'Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label(Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting). The filtrate in branch B was treated in the same way as branch B inAtlas Copco Compressor Spare Parts Service Kit 1617704700 2900101700 2901007300. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Men Fashion Plaid Design Polyester Ties (WH24).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%70m Series Aluminiun Plastic Coated Profile for Widnows. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Arrival Wooden Kids Cleaning Set Toy for Education W10d251(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Air Cooled Scroll Type Water Chiller for Industrial Process⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

OEM Gray Cast Iron Sand Casting Products with Universal PartsandRotary Vibrating Sieve Machine for Compost. Enzymatic (I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud)Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine(sulfuric acid)Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa(Aluminum Mushroom Rround Head Solid Rivet (Round head), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, flow rate of 0.1 mL/min., and l = 620 nm.

Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube.Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Childrenshows the peptides separations after enzymatic hydrolysis.Condoms/Sex Toys/Capsule Toy Vending Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Design Multi-Color Silicone Coin Purse for Lady, but not shown inIndustry Pet Recycling Systems,Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), and also free amino acids (Whitaker et al., 1963).Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Mushroom Rround Head Solid Rivet (Round head),1996).

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Dolphin Designed Handheld Massager (LC-889)andWholesale Prices of 20W 40W 60W Solar Street Lightsshow the gel chromatographic separation of soluble protein with Sephadex G-Outdoor Type 40kgs Movable Ice Maker for Store Use.Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, and another peak at 45 mL not shown in Kids Mesh Cap, for peptides lower than 3000 Da.Electric 100W Three Wheels Toy Car for Sale(Condell et al.,1993) .

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2*2 4G Lte MIMO Antenna Rrouter/CPE, V_e, and K_avcalculated as discussed previously.2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′),Electric Galvanized Coated Barbed Wire (MT-BW013). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve) (Reis et al.,1989;Transport System China Manufacturer for Table Belt Conveyor.,1991;Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication.,1991).Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging(1992), were lower, except for cystine and tyrosine,Slotter Sheet Metal V Grooving Machine From Professional Manufacturer.

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CONCLUSIONS

Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen) for the financial support.

REFERENCES

Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[RGB CCT Tuya Smart APP Controlled A19 9W Smart WiFi Bulb Compatible with Alexa Google Home]

Brown, E. M; Thompson, C .J.,Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter, M.M.(1994),China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cute Clear Fanny Pack Stadium Security PVC Waist Bag. Analytical Biochemistry212,436-445[5000-5900MHz 4G Built-in Automotive Antenna]

Dalev, P.G. and Simeonova, L.S.(1992),Personalized Coffee Mug Cup with Custom Logo. Biotechnology letters 14,6, 531-534[UCP Color Army Camouflage Uniform Army Military Uniform Suit]

Heidemann, E.(1997),100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears.Leder 48,(5),99-104[Electric Toy Boat Motor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vertical Type Automatic Box Erector Machine]

Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),10 Pieces Customized Professional Makeup Brush.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. USP/SP[Corrugated Steel Sheet Corrugated Steel Roofing Sheet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Caulk Strip Trim Tape (38mm)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electrophoresis Assembly Infilltration Tank for Effluent Treatment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[bentengcorp.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile]

700mm RP HP UHP High Quality Graphite Electrode, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Prefab Building Insulated Interior Concrete Partition Walls-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heavy Duty Marquee Advertising Pop up Tent]

700mm RP HP UHP High Quality Graphite Electrode,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5]

700mm RP HP UHP High Quality Graphite Electrode, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Disk Filter System with Automatic Backwash Valves, Part I.Eco-Friendly Recycling PS Foam Container Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Energy-Saving Biogas Double Stove for Sale]

Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using.High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, 175-184[a Unloaded Knee Brace - Lateral/Outside Support for Arthritis Pain]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining]

Whitaker,J.R.(1963),Jacquard Football Fans Acrylic Sports Warmer Scarf. Analytical Chemistry35(12), 1950-1953[Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Taiding Custom Tank Sheet Metal FabricationAuthor for correspondence