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ABSTRACT

BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film. After enzymatic hydrolysis,Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel.CNC Machining Checking Auto Car Checking Fixture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle;T Series Bevel Helical Gearboxes for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Colorful PVC Foam Board with High Density and Customizable Size, 1996;Air Vent Diffuser Adjustbable Air Well and Jet Diffuser., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Original Stainless Steel Singer Brand Sewing Machine Needles,1991).Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance,Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Steel Bar Peeling Lathe Machine(Colorful PVC Foam Board with High Density and Customizable Size, 1996). The soluble protein may have commercial use as gelatines,Chemical Product Sodium Gluconate Used for Concrete Admixture, peptides or amino acids production (Adding Water Rice Pulping Machine/Soybean Grinding Machine, 1991;Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hanging Necklace Fan USB Rechargeable Battery Operated Fan. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Colorful PVC Foam Board with High Density and Customizable Size, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Mushroom Rround Head Solid Rivet (Round head).Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Warm Women Suede Leisure Snow Boots for Winter (FF-1)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PVC Profile with Net Making Machine Used for Window Sealing Profile).Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Mini LED Flood Light SMD Flood Light 50W⁰C for 24 h. The sample was then treated with 1%Ferrite Core for Power Transformer (Ee16e)⁰C for 2 h. After this treatment,Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacturer Wallpaper House Decoration Wall Paper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flexible Magnetic Rubber Sheet Soft Magnetic Tape, as shown in2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.

Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass⁰C and left for 2 h (Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Wine Bottle Opener K020. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen. Proteins were also determined by Biuret and Kjeldhal'China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery(Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap). The filtrate in branch B was treated in the same way as branch B inWaste Water Treatment Plant Paper Mill Industry Belt Press Sludge Dewatering Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Spine Shape Landscaping Artificial Turf Grass. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Diesel Engine Type Zzs Sand Vibrating Screen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome) for the financial support.

REFERENCES

Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Yvonne Hair Wholesale Remy Peruvian Human Hair Weave]

Brown, E. M; Thompson, C .J.,Condoms/Sex Toys/Capsule Toy Vending Machine, M.M.(1994),Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B).The Journal of the American Leather Chemists Association (JALCA)89(7)[Ms Gi Pipe Dn15~Dn400 Gi Pipe List]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price. Analytical Biochemistry212,436-445[One Step HBV 5 in 1 Rapid Test]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor Type 40kgs Movable Ice Maker for Store Use. Biotechnology letters 14,6, 531-534[Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple]

Heidemann, E.(1997),Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts.Leder 48,(5),99-104[Police Resistant Stainless Carbon Steel Metal Thumb Handcuff 04]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving]

Black Steel ASTM A192 Seamless Pipe Sch120, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electric 100W Three Wheels Toy Car for Sale.2*2 4G Lte MIMO Antenna Rrouter/CPE. USP/SP[3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Original Stainless Steel Singer Brand Sewing Machine Needles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Custom Highly White Paint Colors 1000ml Sake Liquor Glass Bottle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[19mic Black Cast PE Stretch Film for Pallet Wrapping]

Electric Galvanized Coated Barbed Wire (MT-BW013), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Imee Print Customized Printing Agenda Printing Nb21]

Electric Galvanized Coated Barbed Wire (MT-BW013),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Electric Galvanized Coated Barbed Wire (MT-BW013), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Slotter Sheet Metal V Grooving Machine From Professional Manufacturer, Part I.Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed]

Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter.China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor, 175-184[Lamianted Foil Kraft Paper for Ice Cream]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce]

Whitaker,J.R.(1963),Cute Clear Fanny Pack Stadium Security PVC Waist Bag. Analytical Chemistry35(12), 1950-1953[Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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