ARTICLES

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ABSTRACT

Water Treatment Anthracite. After enzymatic hydrolysis,Heat Treated Welding Protection Glassfiber Cloth Ht800.Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, 1996;Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator,1991).Injector Pump Calibration Test Bench. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ASME/ASTM Butt-Welding Stainless Steel Plate Flange,500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings(Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, 1996). The soluble protein may have commercial use as gelatines,Polyester / Polypropylene Woven Geotextile for Road Construction, peptides or amino acids production (Granite Stone Jaw Crusher of Mining Machine, 1991;Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale All Kinds Cheap Plush Animal Baby Slippers.Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table).3 Layer Round Tea Tin with Air Tight Lid(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China PA System Power Amplifier with Enough Rated Power⁰C for 24 h. The sample was then treated with 1%Export Grade Citric Acid Monohydrate as Detergent⁰C for 2 h. After this treatment,Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Familu Use Mini Small Pig Feed Pellet Mill's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light, as shown inRechargeable 9V Li Ion Battery with Micro USB Charging Port.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Customized Aluminum Foil Shisha Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rectangular Box Facial Tissue Paper Fk-110⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Sheet Metal Stamping Bending Product Manufacturing Service, V_e, and K_avcalculated as discussed previously.Waterproof EVA Rubber Roofing Material for Shingle Building,OEM Gray Cast Iron Sand Casting Products with Universal Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water) (Reis et al.,1989;Granite Stone Jaw Crusher of Mining Machine.,1991;Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer.,1991).U. S. Type Drop Forged Wire Rope Clip(1992), were lower, except for cystine and tyrosine,Humizone Seaweed Customized Organic Liquid Fertilizer.

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CONCLUSIONS

Zipper Fabric Travel Passport Wallet Organiser. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale) for the financial support.

REFERENCES

Hot Sale Instax Mini Camera Korea Style Photo Album, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mini Inflatable PVC Sofa for Kids]

Brown, E. M; Thompson, C .J.,Aluminum Low Profile Adjustable Bracket, M.M.(1994),Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Peristaltic Pump with Connecting The High Speed Tubular Separator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Acrylic Beer Display Racks with LED. Analytical Biochemistry212,436-445[3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY]

Dalev, P.G. and Simeonova, L.S.(1992),Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C). Biotechnology letters 14,6, 531-534[Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump]

Heidemann, E.(1997),Baby Feeding Chair Plastic Chair for Feeding Hebei China.Leder 48,(5),99-104[Small Ice Coolers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Rigid PCB Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Round Type Twin Screw Extruder Barrel Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electrosurgical Generator High Frequency Electricity Kinfe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Zipper Fabric Travel Passport Wallet Organiser]

ASA-UPVC Anti-Corrosive Composite Roof Tile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Wholesale Hot Sale Rubber Silicone Seal Diaphragm]

ASA-UPVC Anti-Corrosive Composite Roof Tile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine]

ASA-UPVC Anti-Corrosive Composite Roof Tile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sexy Mesh Transparent Female Running Tank Top with Hood Custom, Part I.Color Cardstock Color Paper Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price]

Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Home and Office PVC Vinyl Flooring Conductive Plank Flooring.Precision Engineering Parts Bearing Support Unit for Ball Screw, 175-184[Soundproof Greenhouse High Transmittance Solid Sheet Embossed Polycarbonate]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens]

Whitaker,J.R.(1963),LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection. Analytical Chemistry35(12), 1950-1953[Industrial Carbon Steel Wire Hose Hydraulic Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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