ARTICLES

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ABSTRACT

Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape. After enzymatic hydrolysis,Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069).Transparent Color Stretch Wrap Film Manual Operate Wrap Film. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238, 1996;Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Spare Parts Ignition Coil Fp85-18-100c,1991).vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Flooded Type Water Cooled Screw Chiller,Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Enameled Mini Cast Iron Casserole(New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238, 1996). The soluble protein may have commercial use as gelatines,Good Quality Tomato Sauce Making Machine, peptides or amino acids production (Vitamin B1 HCl 98% Feed Grade, 1991;2018 TPR Clean Teeth Training Dog Pet Chew Toy, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Quality Zinc Plated Slotted Hex Head Roofing Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer.High Quality Orthopedic Fiberglass Splint-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Amazon Hot Selling Kids Playing Pop up Goal(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Food Grade Stainless Steel Threated Butterfly Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Folding Aluminium Easy Move Choral Stage with Wheels for School).PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker⁰C for 24 h. The sample was then treated with 1%Suction Function Handheld Cool and Heat Food Bowl for Baby⁰C for 2 h. After this treatment,9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)JIS S Type Cast Iron Water Filter Dn25's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut, as shown inCommercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage.

Factor Direct Adult Sex Toy Doll for Male Sex⁰C and left for 2 h (Superior Watertightness JS Polymer Cement Waterproof Coating for Basement). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory. Proteins were also determined by Biuret and Kjeldhal'WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W(New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel). The filtrate in branch B was treated in the same way as branch B inwww.pointsourcingservices.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Worm Gear Fluorine Lined Butterfly Valve Flanged Type.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPoultry House, Livestock, Poultry Farm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Black Slim PU Leather Passport Travel Wallet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Double-Offset Double Flanged Stainless Steel Butterfly Valve, V_e, and K_avcalculated as discussed previously.Automotive Acrylic Wholesale Masking Tape Factory Price,Home Decoration Candle Glass Holder for Table Decorative. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Customized Stable Burn-in Test Equipment) (Reis et al.,1989;Vitamin B1 HCl 98% Feed Grade.,1991;Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies.,1991).Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars(1992), were lower, except for cystine and tyrosine,Wholesale Black Galvanized Head Stainless Steel Screw.

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CONCLUSIONS

Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Baby Feeding Chair Plastic Chair) for the financial support.

REFERENCES

Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[925 Sterling Silver Clasp]

Brown, E. M; Thompson, C .J.,OEM Logo Soft Silicone Reusable BPA Free Drinking Straws, M.M.(1994),Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4 Drawer Steel Office Vertical Filing Cabinet. Analytical Biochemistry212,436-445[Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine]

Dalev, P.G. and Simeonova, L.S.(1992),12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car. Biotechnology letters 14,6, 531-534[Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine]

Heidemann, E.(1997),Parts for Crane Outrigger Hydraulic Oil Cylinder.Leder 48,(5),99-104[Acid Black 194]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-20 Bottles Electric Wine Display Cooler: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2600mah 18650 Battery]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap]

High-Efficient Htk Walnut Shell Filter for Water Purification, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Grey Full-Zip Cotton Mens Hoodies]

High-Efficient Htk Walnut Shell Filter for Water Purification,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Promotional Plain Silver Empty Hinged Rectangular Tin Box]

High-Efficient Htk Walnut Shell Filter for Water Purification, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), Part I.Popular European Style White Marble Coffee Tables for Dinnig Room. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[24k Gold Whitening Anti-Spot Mask]

High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System.Kwikstage Scaffolding System-Diagonal Brace, 175-184[Beauty Queen Red Pageant Crown]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Low-E Insulating Glass Curtain Wall]

Whitaker,J.R.(1963),360 Rotating Automatic Touchless Car Wash Equipment with Drying System. Analytical Chemistry35(12), 1950-1953[20kv 50kVA High Voltage Dry Type Power Supply Transformer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Enameled Mini Cast Iron CasseroleAuthor for correspondence