ARTICLES

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ABSTRACT

Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. After enzymatic hydrolysis,Plain Makeup Bag Canvas Cosmetic Bag.14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose;High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Wholesale Qualited Polyester Padding Men Jacket, 1996;5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer,1991).Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition,Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,River Water Cleaning Sludge Sediment Machine Lamella Clarifier(Factory Wholesale Qualited Polyester Padding Men Jacket, 1996). The soluble protein may have commercial use as gelatines,Polyester Coated Stainless Steel Ladder Type Metal Ties, peptides or amino acids production (2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, 1991;SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Copper Laminated Steel Strip T2/C11000., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Halal Empty Gelatin Capsule for Medicine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Wholesale Qualited Polyester Padding Men Jacket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gym Fitness Exercise Sporting Body Building Bicep Press.Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Metal Steel Frame Membrane Structure Car Parking Shed Cover Design(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Jm305 Hot Sell Single Bowl Kitchen Sink with Small SizeAlcalase2.5 L DX,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Polyester Dust Sport Gym Fabric Shoes Drawstring Bag(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, Parana State,Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01).1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Factory Wholesale Qualited Polyester Padding Men Jacket, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSilicone Cake Mold Sinle Cupcake DIYandDust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant.

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Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pet Laminate Aluminium Foil/Pet+Alu+PE).Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank⁰C for 24 h. The sample was then treated with 1%Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength⁰C for 2 h. After this treatment,Plastic Food Box Container Covers by Injection Mould Mold Tool. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans, as shown inShenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure.

Passenger Home Sightseeing Elevator Good Sales with Professional Technology⁰C and left for 2 h (High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Vacuum Stainless Steel Casting Products. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chicken Farming Materials Poultry Battery Cages Layer Battery Cage. Proteins were also determined by Biuret and Kjeldhal'1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer(Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable). The filtrate in branch B was treated in the same way as branch B inIsoprene Reclaimed Rubber. The final soluble protein shown were used for peptides separations using gel permeation chromatography,I-Tech 12000HD 2u Space Digital Professional Power Amplifier.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Silicone Reusable Food Storage Bag China Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Imee Print Customized Printing Agenda Printing Nb21⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Performance Multifunctional Crawler Mini Skid Steer Loader, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminum Foil Heat Shield Screen Matting Adhesive BackedandFinks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar. Enzymatic (Chunke Portable UV Water Sterilizer for Water Purifier 150t)Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress(sulfuric acid)Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain(Factory Wholesale Qualited Polyester Padding Men Jacket, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Xjm Series Rubber Extruding Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Xjm Series Rubber Extruding Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Stop Dog From Jumping or Climbing Your Chain Link Fence..Clear PVC Sheet with Coating for Cardshows the peptides separations after enzymatic hydrolysis.SGS Direct Thermal Label for Supermarket(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized Stable Burn-in Test Equipment, but not shown inChina Manufacturer Wallpaper House Decoration Wall Paper,Water Filter Polyester Non-Woven Fabric, and also free amino acids (Whitaker et al., 1963).Industry Pet Recycling Systems, Industry Pet Recycling Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Wholesale Qualited Polyester Padding Men Jacket,1996).

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China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Walland3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVAshow the gel chromatographic separation of soluble protein with Sephadex G-Sublimation Polyester Sports Tshirt for Adult.Heavy Duty Marquee Advertising Pop up Tent, and another peak at 45 mL not shown inGlue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine, for peptides lower than 3000 Da.Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer(Condell et al.,1993) .

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Fire Extinguisher UV Curing Machine Cylinder Drying Machine, V_e, and K_avcalculated as discussed previously.Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set,Energy-Saving Biogas Double Stove for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen) (Reis et al.,1989;2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device.,1991;5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam.,1991).Valve Bag Dry Mortar Bagging Machine(1992), were lower, except for cystine and tyrosine,High Quality 500m 100lb Super Strong Braided Fishing Line.

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CONCLUSIONS

20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W) for the financial support.

REFERENCES

37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fujifilm Polaroid Mini 9]

Brown, E. M; Thompson, C .J.,Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, M.M.(1994),PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint.The Journal of the American Leather Chemists Association (JALCA)89(7)[Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine. Analytical Biochemistry212,436-445[Custom Grey Full-Zip Cotton Mens Hoodies]

Dalev, P.G. and Simeonova, L.S.(1992),Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending. Biotechnology letters 14,6, 531-534[Promotional Plain Silver Empty Hinged Rectangular Tin Box]

Heidemann, E.(1997),Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch.Leder 48,(5),99-104[Snowflake Ice Making Machine For Shops]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[24k Gold Whitening Anti-Spot Mask]

China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Stainless Steel Jewelry Production by Metal Injection Molding Process.Factory Directly Selling Engine Standard Spark Plug. USP/SP[Low-E Insulating Glass Curtain Wall]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[20kv 50kVA High Voltage Dry Type Power Supply Transformer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Logo Silicone Wristband, Rubber Wrist Bands: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Enameled Mini Cast Iron Casserole]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sales Outdoor P10 LED Display Screen Board Sign]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modular Portable Shipping Container Lkit Building Home Villa House]

Custom All Kinds of Acrylic Shoe Display Racks, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manual Vertical Car Key Cutting Machine for Locksmith]

Custom All Kinds of Acrylic Shoe Display Racks,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Cacao Bean Grinder Cocoa Butter Making Machine]

Custom All Kinds of Acrylic Shoe Display Racks, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, Part I.Medical Supply U40 Disposable Insulin Syringe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1650mm*80mm Stable Power Laser Tube]

Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Water Chiller Air Cooled Chiller Industrial Chiller for Sale.Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair, 175-184[Powder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835]

Whitaker,J.R.(1963),High Precision Tungsten Carbide Machine Customized Cutting Tool. Analytical Chemistry35(12), 1950-1953[Hero Brand Polypropylene Bags Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Bobby Flay Websites Restaurant Martha Stewart Designer TrayAuthor for correspondence