ARTICLES

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ABSTRACT

Bearing Stand Type Cam Follower. After enzymatic hydrolysis,Hot Sale Electric LED light Aroma Diffuser.Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306;Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.7PCS RGBW LED Plastic PAR Light Wall Washer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, 1996;Kindergarten Playground Preschool Kids Plastic Slide and Swing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks,1991).Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Railway Freight Wagon Spare Parts Casting and Forging for Bogie,Rainsky Ceiling Mounted Stainless Steel Shower Heads,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Sizes Acrylic Shoes Storage Display Box(750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, 1996). The soluble protein may have commercial use as gelatines,High Quality Casting Cover Aluminum Die Casting Products, peptides or amino acids production (Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags, 1991;304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts.Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:304 316 Stainless Steel Grooved Cross for Water(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, Parana State,2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover.Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustrial Rubber Nylon Conveyor Belting Automotive PartsandAgricultural Irrigation Centrifugal Double Suction Diesel Water Pump.

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Acm Dewatering Twin Screw Extruder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ASTM A240 321 Stainless Steel Sheet Price Per Kg).Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tumble Belt Type Shot Blasting Machine for Iron Castings Parts⁰C for 24 h. The sample was then treated with 1%Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple⁰C for 2 h. After this treatment,Good Quality Good Price China Plastic Injection Molding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pet Hair Remover Glove for Dog, Grooming Gloves Brush's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, as shown in CNC Lathe Machine Milling.

Alumina Balls for Hydogen Peroxide⁰C and left for 2 h (Bending Sheet Metal Custom-Made Aluminum Bracket). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anti-High Pressure Insulated Pliers with PVC Dipped Handles. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Selling Electronical Blue Pet Plastic Release Film Price. Proteins were also determined by Biuret and Kjeldhal'DIN127 Stainless Steel Spring Lock Washer(Pneumatic Air Driven Liquid Booster Pump). The filtrate in branch B was treated in the same way as branch B in2.4G Wireless Ap 200MW X200/Wireless Network/Wireless Access Point/WiFi Ap/WiFi Bridge/WiFi Signal Booster. The final soluble protein shown were used for peptides separations using gel permeation chromatography,29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Condition Hot Sale Automatic Animal Feed Cooler Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAhd 720p 7 Quad Screen Monitor Car Rearview Camera System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Garlic Red Mesh Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ground Finish Molybdenum Tzm Rod, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Engine Parts Rubber High Quality Timing Belt 111ru25andTransparent/Clear BOPP Jumbo Roll. Enzymatic (Yashi 19 270degree Pyramid Hologram Display Showcase Attractive)Chenille Microfiber Mop Head(sulfuric acid)Custom Waterproof Pizza Sign Large Advertising Signboard(750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PVC Shrink Sleeve Label for Drinking Water Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PVC Shrink Sleeve Label for Drinking Water Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4.White HPL 600*600mm Coffee Tableshows the peptides separations after enzymatic hydrolysis.Galvanized Square Wire Mesh, Metal Window Screen(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFull Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant, but not shown inAir Cooled Scroll Type Water Chiller for Industrial Process,Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, and also free amino acids (Whitaker et al., 1963).2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm,1996).

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CONCLUSIONS

Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory) for the financial support.

REFERENCES

Linen Quiltcover Set with Quilting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Dip Galvanized Erw Steel Pipe]

Brown, E. M; Thompson, C .J.,2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, M.M.(1994),Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality PVC Resin with Low Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Exfoliating Shower Hemp Bath Sponge Pad]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Teerte Square Flat Head Self Tapping Screw. Analytical Biochemistry212,436-445[Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell]

Dalev, P.G. and Simeonova, L.S.(1992),Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard. Biotechnology letters 14,6, 531-534[Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment]

Heidemann, E.(1997),Bto 22 Electric Galvanized Razor Concertina Wire for Military.Leder 48,(5),99-104[I-Beam Steel in Line with International Standards]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glass Lined Reactor for Chemical Factory]

Ysdcnc Manual Sheet Metal Plate Bending Roller Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights.Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. USP/SP[2D LED Rope Lighting Motif Lights for Christmas Decoration]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Selling Military Large Event Marquee Tent Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality Xanthan Gum Application of Food with Best Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Xr18k Tungsten Carbide Die]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing]

High-Quality Pre-Seasoned Cast Iron Wok, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[JIS S Type Cast Iron Water Filter Dn25]

High-Quality Pre-Seasoned Cast Iron Wok,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears]

High-Quality Pre-Seasoned Cast Iron Wok, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, Part I.Marine High Visibility Solas Reflective Tape. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Strength Rigid PVC Plastic Foam Board for FRP]

24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444).Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, 175-184[Ca Fast Response High Quality Fire Extinguisher Ball Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker]

Whitaker,J.R.(1963),Magic Tape Hook and Loop Fasteners. Analytical Chemistry35(12), 1950-1953[ASME/ASTM Butt-Welding Stainless Steel Plate Flange]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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