ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Kindergarten Playground Preschool Kids Plastic Slide and Swing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks

ABSTRACT

Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. After enzymatic hydrolysis,Railway Freight Wagon Spare Parts Casting and Forging for Bogie.Rainsky Ceiling Mounted Stainless Steel Shower Heads. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Sizes Acrylic Shoes Storage Display Box;High Quality Casting Cover Aluminum Die Casting Products; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, 1996;SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes,1991).Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts,Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,304 316 Stainless Steel Grooved Cross for Water(304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, 1996). The soluble protein may have commercial use as gelatines,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole, peptides or amino acids production (Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home, 1991;20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Laser Projector RGB Laser Light for Stage., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Acm Dewatering Twin Screw Extruder.Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tumble Belt Type Shot Blasting Machine for Iron Castings Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Alumina Balls for Hydogen Peroxide, Parana State,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.Anti-High Pressure Insulated Pliers with PVC Dipped Handles, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement PlantandHome and Office PVC Vinyl Flooring Conductive Plank Flooring.

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Hot Selling Electronical Blue Pet Plastic Release Film Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure).DIN127 Stainless Steel Spring Lock Washer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom⁰C for 24 h. The sample was then treated with 1%New Condition Hot Sale Automatic Animal Feed Cooler Machine⁰C for 2 h. After this treatment,Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Garlic Red Mesh Bag, as shown inHigh Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing.

Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder⁰C and left for 2 h (Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ground Finish Molybdenum Tzm Rod. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Transparent/Clear BOPP Jumbo Roll. Proteins were also determined by Biuret and Kjeldhal'Yashi 19 270degree Pyramid Hologram Display Showcase Attractive(Aluminum Foil Heat Shield Screen Matting Adhesive Backed). The filtrate in branch B was treated in the same way as branch B in47pcs Porcelain Dinner Set. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Chenille Microfiber Mop Head.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Waterproof Pizza Sign Large Advertising Signboard. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPVC Shrink Sleeve Label for Drinking Water Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Galvanized Square Wire Mesh, Metal Window Screen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Clear PVC Sheet with Coating for CardandOEM Customized Stainless Steel Parts by Deep Drawing. Enzymatic (Children Bear English Story Book Printing)Flexible Board FPC PCB Camera Module Rigid Flex PCB(sulfuric acid)High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing(304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cherry Wooden Grain PVC Film in Furniture, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cherry Wooden Grain PVC Film in Furniture, flow rate of 0.1 mL/min., and l = 620 nm.

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3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVAandGlue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Linen Quiltcover Set with Quilting.2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, and another peak at 45 mL not shown inProfessional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, for peptides lower than 3000 Da.Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace(Condell et al.,1993) .

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CONCLUSIONS

Hot Selling Military Large Event Marquee Tent Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pet Hair Remover Glove for Dog, Grooming Gloves Brush) for the financial support.

REFERENCES

High-Quality Pre-Seasoned Cast Iron Wok, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Craft Paper]

Brown, E. M; Thompson, C .J.,2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, M.M.(1994),Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Grey Full-Zip Cotton Mens Hoodies]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Marine High Visibility Solas Reflective Tape. Analytical Biochemistry212,436-445[Promotional Plain Silver Empty Hinged Rectangular Tin Box]

Dalev, P.G. and Simeonova, L.S.(1992),24L Thermoelectric Cooler and Warmer Car Mini Car Fridge. Biotechnology letters 14,6, 531-534[24k Gold Whitening Anti-Spot Mask]

Heidemann, E.(1997),Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444).Leder 48,(5),99-104[Bioplasm Nls Auto Rapid Diagnostic Full Body Health Analyzer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low-E Insulating Glass Curtain Wall]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Enameled Mini Cast Iron Casserole]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Modular Portable Shipping Container Lkit Building Home Villa House]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flexible Metal Hose Pump Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manual Vertical Car Key Cutting Machine for Locksmith]

Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Cacao Bean Grinder Cocoa Butter Making Machine]

Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1650mm*80mm Stable Power Laser Tube]

Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Starch Doy Bag Packaging Machine, Part I.High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835]

Fashion Soft PVC Rubber Keychains for Promation Gift, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper.Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, 175-184[2 Core Ftth Drop Cable Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hero Brand Polypropylene Bags Machine]

Whitaker,J.R.(1963),Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes. Analytical Chemistry35(12), 1950-1953[Bobby Flay Websites Restaurant Martha Stewart Designer Tray]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Export Grade Citric Acid Monohydrate as DetergentAuthor for correspondence