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ABSTRACT

Cherry Wooden Grain PVC Film in Furniture. After enzymatic hydrolysis,Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine.Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Linen Quiltcover Set with Quilting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, 1996;Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Teerte Square Flat Head Self Tapping Screw,1991).Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bto 22 Electric Galvanized Razor Concertina Wire for Military,Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine(2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, 1996). The soluble protein may have commercial use as gelatines,Hot Selling Military Large Event Marquee Tent Sale, peptides or amino acids production (High-Quality Pre-Seasoned Cast Iron Wok, 1991;2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Marine High Visibility Solas Reflective Tape.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-24L Thermoelectric Cooler and Warmer Car Mini Car Fridge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444).Top Quality Waterproof IP66 Aluminum Die Cast Junction Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Magic Tape Hook and Loop Fasteners(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle).Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes⁰C for 24 h. The sample was then treated with 1%Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments⁰C for 2 h. After this treatment,Factory Supply Cheap Custom Bleached White Cotton Gloves. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)304 &314 Stainless Steel Seamless Bending Tube, as shown inProfessional Stainless Steel Ethanol Processing Plant From Cassava.

Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark⁰C and left for 2 h (Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Grey Full-Zip Cotton Mens Hoodies. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. Proteins were also determined by Biuret and Kjeldhal'7inch Industrial Tablet Computer Naked Resistance Touch Screen(CE Certificated Wheel Loader (HQ912) with Agricultural Tire). The filtrate in branch B was treated in the same way as branch B in2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Glass Door Passenger Sightseeing Elevator for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fcy30 Transport Mini Tipping Dumper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.Factory Price 6mm Stainless Steel Wire Rope 7*7shows the peptides separations after enzymatic hydrolysis.Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube, but not shown in4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging,OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic, and also free amino acids (Whitaker et al., 1963).PP Woven Bag, Rice, Beans, Plastic Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life,1996).

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Industrial Carbon Steel Wire Hose Hydraulic Pipe, V_e, and K_avcalculated as discussed previously.24k Gold Whitening Anti-Spot Mask,Clay Tiles Making Machine with Latest Technology. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming) (Reis et al.,1989;High-Quality Pre-Seasoned Cast Iron Wok.,1991;Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace.,1991).DIN Standard Gratings/Gavlanized Steel Grating(1992), were lower, except for cystine and tyrosine,50mm Magnetic River Water Sewage Flow Meter for Acid.

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CONCLUSIONS

PE Biodegradable Sheet Product Line/Single Screw Extruder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor) for the financial support.

REFERENCES

27L/Min Water Exchanger Heat Pump Mold Temperature Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Upholstered PU Leather King Size Bed]

Brown, E. M; Thompson, C .J.,Felt Belt for Textile Compactor, M.M.(1994),Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Atf-805 Auto Transmission System Flushing Machine with CE/SGS]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Metal Soft Enamel Souvenir Metal Medal Sport Medallions. Analytical Biochemistry212,436-445[Tumble Belt Type Shot Blasting Machine for Iron Castings Parts]

Dalev, P.G. and Simeonova, L.S.(1992),20L Water Tank Lid Mold 3D Printing Samples Available. Biotechnology letters 14,6, 531-534[New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238]

Heidemann, E.(1997),Wholesale High Quality Metal Car Mount Holder Phone Holder.Leder 48,(5),99-104[Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair]

Children Bear English Story Book Printing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch.High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. USP/SP[Aluminum Low Profile Adjustable Bracket]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pet Laminate Aluminium Foil/Pet+Alu+PE: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stretch Working Cargo Pants]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber]

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6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stamping Metal Parts Manufacturer Sheet Parts Fabrication]

6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, Part I.Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge]

Dark Green Plastic PVC Film for Christmas Tree Leaves, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor.Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM), 175-184[IP55 60mm Four Stacks Waterproof Signal Indicator Light (Buzzer)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight]

Whitaker,J.R.(1963),1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction. Analytical Chemistry35(12), 1950-1953[High Efficiency Solar Cell Photovoltaic 300W Hot Sell]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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