ARTICLES

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ABSTRACT

Crop Fertilizer Agriculture Nh4cl Ammonium Chloride. After enzymatic hydrolysis,Wholesale High Pressure 80L Nitrogen Gas Cylinder.10mm Red Green Yellow Warning China Supplier Signal Lamp. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Phillip Countersunk Head Screw Hot DIP Galvanized, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, 1996;Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Heat Shrink Automatic Flow Wrapping Packing Machine,1991).Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ductile Iron Standard Brackets Pipe Hanger,Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper(Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, 1996). The soluble protein may have commercial use as gelatines,5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building, peptides or amino acids production (High Quality Sheet Metal Bending Roll Forming Machine, 1991;Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Frame Type Rubber Hydraulic Press Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Kitchen Metal Dish Storage Rack (JP-SC984C). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming.AVR Se350 Automatic Voltage Regulator for Marathon Alternator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Line Scan Uvss System Portable Under Vehicle Surveillance).New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck⁰C for 24 h. The sample was then treated with 1%Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module⁰C for 2 h. After this treatment,ADSS Newest Ultra Therapy Skin Tightening 3D Hifu. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, as shown in TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank.

High Quality Ceramic Chrome Hybrid Bearing Oil⁰C and left for 2 h (Electric 100W Three Wheels Toy Car for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder. Proteins were also determined by Biuret and Kjeldhal'Digger Arm Small Wheel Loader (HQ908) with CE(2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life). The filtrate in branch B was treated in the same way as branch B in4 Oz 8oz 16 Oz 32 Oz Glass Mason Jar with Metal Lid/ Wide Mouth Mason Jam Jar. The final soluble protein shown were used for peptides separations using gel permeation chromatography,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWater Based Emulsion Paint Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Cnm Single Strand Anchor Coupler(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/18X16mesh Fiberglass Window Screen Insect Screen, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for HomeandSterile Dsiposable Syringe for Dental 12ml. Enzymatic (Foldable Bed Military Cot for Camping)P5 960*960mm LED Display Screen SMD Indoor /Outdoor(sulfuric acid)2019 New Advertising Inflatables Air Walking Backpack Ball(Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Side Pack Manual Gas Engine Power Brush Cutter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Side Pack Manual Gas Engine Power Brush Cutter, flow rate of 0.1 mL/min., and l = 620 nm.

Punching Bending Welding Steel Work Fabrication.Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabricationshows the peptides separations after enzymatic hydrolysis.Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Food Cookies Cake Cartoning Machine for Big Size Carton Box, but not shown inEco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws,Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, and also free amino acids (Whitaker et al., 1963).1 Ton Chain Pulley Block with Geared Trolley. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse,1996).

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IEC 62196-2 Type 2 EV Charging 32A Male Plug, V_e, and K_avcalculated as discussed previously.PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter,180W 1850mm*80mm Reci Laser Tube. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Flower Design Custom Made Silk Scarf) (Reis et al.,1989;High Quality Sheet Metal Bending Roll Forming Machine.,1991;Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll.,1991).OEM Factory Balloon Padding Textile Machinery(1992), were lower, except for cystine and tyrosine,NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy.

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CONCLUSIONS

Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building) for the financial support.

REFERENCES

Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Interior Design For Bar]

Brown, E. M; Thompson, C .J.,Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, M.M.(1994),Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-22*30mm 12SMD 5050 LED Reading Light for Car. Analytical Biochemistry212,436-445[Yashi 19 270degree Pyramid Hologram Display Showcase Attractive]

Dalev, P.G. and Simeonova, L.S.(1992),SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital. Biotechnology letters 14,6, 531-534[Portable Electric Concrete Vibrator with 18000 High Rotation Speed]

Heidemann, E.(1997),Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness.Leder 48,(5),99-104[Gift Box For Garment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom High Precision Micro Machining Service Brass Micromachining Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Microfiber Towel Quick-Drying Beach Towels Spring/Autumn Swimming SPA Towel for Adult]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Three Side Sealing Facial Mask Bag Making Machine]

P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt]

P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply]

P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, Part I.Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple]

Printed Wristband Cotton Wristband RFID Woven Wristband, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles.Dolphin Designed Handheld Massager (LC-889), 175-184[2 Inch Rope Clamp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding]

Whitaker,J.R.(1963),Real Leather Wallet Mens Designer Leather Wallets. Analytical Chemistry35(12), 1950-1953[5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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