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ABSTRACT

0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming. After enzymatic hydrolysis,AVR Se350 Automatic Voltage Regulator for Marathon Alternator.Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147;High Strength Rigid PVC Plastic Foam Board for FRP; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, 1996;Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould,1991).LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels,Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module(4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, 1996). The soluble protein may have commercial use as gelatines,ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, peptides or amino acids production (Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, 1991;Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Ceramic Chrome Hybrid Bearing Oil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Digger Arm Small Wheel Loader (HQ908) with CE.15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Foldable Bed Military Cot for Camping(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor).P5 960*960mm LED Display Screen SMD Indoor /Outdoor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 New Advertising Inflatables Air Walking Backpack Ball⁰C for 24 h. The sample was then treated with 1%Side Pack Manual Gas Engine Power Brush Cutter⁰C for 2 h. After this treatment,Punching Bending Welding Steel Work Fabrication. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, as shown inSGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment.

1 Ton Chain Pulley Block with Geared Trolley⁰C and left for 2 h (High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Powder Coating Polyester Resin with Factory Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Plastic Part Hardware Plastic Injection Mold. Proteins were also determined by Biuret and Kjeldhal'Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine( Color Coated Aluminum Coils). The filtrate in branch B was treated in the same way as branch B inOEM Anodizing Precision Sheet Metal Stamping. The final soluble protein shown were used for peptides separations using gel permeation chromatography,IEC 62196-2 Type 2 EV Charging 32A Male Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av180W 1850mm*80mm Reci Laser Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Performance Multifunctional Crawler Mini Skid Steer LoaderandOutdoor Concert Stage Equipment Aluminum Stage Portable Stage. Enzymatic (Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home)22*30mm 12SMD 5050 LED Reading Light for Car(sulfuric acid)SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital(4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness, flow rate of 0.1 mL/min., and l = 620 nm.

Outdoor Cheap Portable Stage Aluminum Truss Stage.Custom CNC Milled Precision Spare Part CNC Pen Automobile Partsshows the peptides separations after enzymatic hydrolysis.One Arm Motorized Multifunction Hospital Equipment for China Supply(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYe3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw, but not shown inTop China Forklift Supplier & Manufacturer,Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, and also free amino acids (Whitaker et al., 1963).P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall,1996).

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Printed Wristband Cotton Wristband RFID Woven Wristband, V_e, and K_avcalculated as discussed previously.High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles,Dolphin Designed Handheld Massager (LC-889). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ID25mm Od86mm Standard Through Hole Slip Ring) (Reis et al.,1989;Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap.,1991;Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve.,1991).Real Leather Wallet Mens Designer Leather Wallets(1992), were lower, except for cystine and tyrosine,ASTM A240 321 Stainless Steel Sheet Price Per Kg.

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CONCLUSIONS

V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp) for the financial support.

REFERENCES

Promotional Plain Silver Empty Hinged Rectangular Tin Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Professional Microfiber Waffle Kitchen Cafe Towels]

Brown, E. M; Thompson, C .J.,Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, M.M.(1994),5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Comfortable Two Piece Women Fitness Clothing for Gym Wear]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm). Analytical Biochemistry212,436-445[Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck]

Dalev, P.G. and Simeonova, L.S.(1992),11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale. Biotechnology letters 14,6, 531-534[H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)]

Heidemann, E.(1997),Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H).Leder 48,(5),99-104[Factory LED Solar Garden Outdoor Landscape Lawn Light Lamp Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe]

Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cantilever Oxygen Plasma Cutter 80.Stainless Steel Expansion Anchor Bolt Steel Zinc Plated. USP/SP[Stainless Steel Expansion Anchor Bolt Steel Zinc Plated]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Load 1600kg Small Machine Room Bed Elevator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Fiber Laser Cutting Machine for Sheet and Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FTTH Embedded Type Sc Fiber Optic Fast Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[8 Channel 4K H. 265 Network Video Recorder Surveillance CCTV System NVR]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industry 0.002mm Accuracy Checking Fixture]

Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Impact Sound Isolation Wall Clip]

Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[AISI 410 Stainless Steel Galvanized Wire for Making Scourer]

Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, Part I.Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024]

High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper.300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, 175-184[Custom Control Center Frame Plastic Injection Molds for Auto Part]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter]

Whitaker,J.R.(1963),BS 5163 Ductile Iron Rising Stem Gate Valve. Analytical Chemistry35(12), 1950-1953[B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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