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ABSTRACT

Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress. After enzymatic hydrolysis,Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain.Xjm Series Rubber Extruding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stop Dog From Jumping or Climbing Your Chain Link Fence.;SGS Direct Thermal Label for Supermarket; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Water Filter Polyester Non-Woven Fabric, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industry Pet Recycling Systems, Industry Pet Recycling Line, 1996;Sublimation Polyester Sports Tshirt for Adult., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Heavy Duty Marquee Advertising Pop up Tent,1991).Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fire Extinguisher UV Curing Machine Cylinder Drying Machine,Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Energy-Saving Biogas Double Stove for Sale(Industry Pet Recycling Systems, Industry Pet Recycling Line, 1996). The soluble protein may have commercial use as gelatines,Valve Bag Dry Mortar Bagging Machine, peptides or amino acids production (High Quality 500m 100lb Super Strong Braided Fishing Line, 1991;20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industry Pet Recycling Systems, Industry Pet Recycling Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine.Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Medical Supply U40 Disposable Insulin Syringe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (0.1mm Tungsten Carbide 2f Single Flute Micro End Mill).Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Water Chiller Air Cooled Chiller Industrial Chiller for Sale⁰C for 24 h. The sample was then treated with 1%Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair⁰C for 2 h. After this treatment,High Precision Tungsten Carbide Machine Customized Cutting Tool. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Maleic Anhydride CAS 108-31-6, as shown inProfessional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould.

2000mj Q Switched for Pigment Removal with Ce Certification⁰C and left for 2 h (High Quality Army Captain Navy Blue Color Hat for Army Tender). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cow Dung Chicken Manure Organic Fertilizer Making Machine. Proteins were also determined by Biuret and Kjeldhal'marble Makeup Brush Set for Travel in 20PCS(Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor). The filtrate in branch B was treated in the same way as branch B inHigh Quality Membrane Panel Cooperative with Delonghi. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Swimming Pool Water Disinfect Ozone Generator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%20kv 50kVA High Voltage Dry Type Power Supply Transformer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avManufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4 Styles Blue Glass Crystal Butt Beads Spiral Plug⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial Activated Carbon Water Filter Industry Water Treatment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Polyester Coated Stainless Steel Ladder Type Metal Tiesand2016 New Generation Sausage Making Machine. Enzymatic (Magnetic Mixer Mixing Tank with Bottom Agitator (MTK))Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent(sulfuric acid)New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)(Industry Pet Recycling Systems, Industry Pet Recycling Line, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Supplier Building Construction Steel Frame (ZY431), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Supplier Building Construction Steel Frame (ZY431), flow rate of 0.1 mL/min., and l = 620 nm.

iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing.China Excavator Radiator /Oil Radiator /Water Radiator Manufactureshows the peptides separations after enzymatic hydrolysis.Lab Equipment portable Fume Hood(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPromotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday, but not shown in380mm Working Area Concrete Diamond Floor Edge Grinder,Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, and also free amino acids (Whitaker et al., 1963).10ton Telescopic Hydraulic Truck Crane. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industry Pet Recycling Systems, Industry Pet Recycling Line,1996).

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CONCLUSIONS

2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Logo Silicone Wristband, Rubber Wrist Bands) for the financial support.

REFERENCES

PRO-Installer Fiber Optic Termination Kit Lk-6016, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Scroll Process Chiller]

Brown, E. M; Thompson, C .J.,Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace, M.M.(1994),Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901).The Journal of the American Leather Chemists Association (JALCA)89(7)[ Head Lamp Fabia Mining Lamp]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[T Series Bevel Helical Gearboxes for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs. Analytical Biochemistry212,436-445[2010 Used Foton Truck Mixer]

Dalev, P.G. and Simeonova, L.S.(1992),Powder Coated H Column Beam Steel Structure Building for Sale. Biotechnology letters 14,6, 531-534[Heat Shrink Automatic Flow Wrapping Packing Machine]

Heidemann, E.(1997),Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor.Leder 48,(5),99-104[Graphite Casting Molds]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polyester Dust Sport Gym Fabric Shoes Drawstring Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nh4cl White Granular Agricultural Grade Ammonium Chloride]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nylon Cable Glands with CE: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Anti-Steal SMC Ditch Gully Grate for Urban Drainage System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine]

X Shaped Extruded Aluminum Window Screen Frames, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3PC Stainless Steel Flanged End Ball Valve 3 Inches 150]

X Shaped Extruded Aluminum Window Screen Frames,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine]

X Shaped Extruded Aluminum Window Screen Frames, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4, Part I.4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Halal Chicken Slaughter Machine for Sale]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Wire and Insulation]

Whitaker,J.R.(1963),New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug. Analytical Chemistry35(12), 1950-1953[12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Electric Galvanized Coated Barbed Wire (MT-BW013)Author for correspondence