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ABSTRACT

High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump. After enzymatic hydrolysis,10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper.300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;BS 5163 Ductile Iron Rising Stem Gate Valve;CNC Aluminum Sheet Press Brake; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ID25mm Od86mm Standard Through Hole Slip Ring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, 1996;Bio Media., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine,1991).Platform Trolley with 150kg Capacity, 4 Castor Wheel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine,LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,All Size Colored Tapping Hex Head Self Drill Screw Wood Screw(Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, 1996). The soluble protein may have commercial use as gelatines,Pre-Engineered Cage Broiler Poultry Farm Building, peptides or amino acids production (Alumina Ball with High Quality, 1991;PVC Textile Fabric for Outdoor Sports Waterproof Backpack, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;White 4*8FT 18mm PVC Foam Board for Cabinets Use., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Powerful Anti-Stress, Strong Flexibility Spoke Wire.Modern American Style Beech Solid Wood Kitchen Cabinet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hero Brand Polypropylene Bags Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire).Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%En1092-1 05 Pn16 Blind Flat Face Flange⁰C for 24 h. The sample was then treated with 1%Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag⁰C for 2 h. After this treatment,Peristaltic Pump with Connecting The High Speed Tubular Separator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Bulb Light with Remote Control RGB Christmas Bulb, as shown inComputerized Single Fishnet Jacquard Circular Knitting Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.12ml Round Shape Empty Clear Nail Polish Glass Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/M4X10 Round Head Core Pulling Aluminum Nail, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

New Shacman F3000 6X4 Truck Trailer Head. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chicken Layer Cage Poultry Cage Chicken Cages) for the financial support.

REFERENCES

Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Floor Lamp_Standard Lamp]

Brown, E. M; Thompson, C .J.,Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention, M.M.(1994),Cylindrical Shape Beer Glass Bottle for Wine Storage.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Injection for OEM Product. Analytical Biochemistry212,436-445[ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024. Biotechnology letters 14,6, 531-534[Powder Coated H Column Beam Steel Structure Building for Sale]

Heidemann, E.(1997),Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event.Leder 48,(5),99-104[Customized 180 Degree Digital Camera Hinge/Camera Hardware /Axis]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Water Based Emulsion Paint Production Line]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Different Thickness High Quality Aluminum Foil for Glass Bottle Lid]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Type Price Prefabricated Roof Tile in Sudan: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Supplier Building Construction Steel Frame (ZY431)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sc-2400 Automatic Multi Function Sosn Side Drilling Machine]

Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Supply 100% Natural Epimedium Extract for Male Health-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park]

Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump]

Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust, Part I.Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Surprise! Range Hood Free! ! ! Juice Cart]

Top10 1080P H. 264 P2p CCTV WiFi IP Camera, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture.Manufacturer Custom Precision Aluminum Die Casting LED Light Housing, 175-184[Jt40 Right Angle Gearbox Bevel Gearboxes Miter Gear Boxes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light]

Whitaker,J.R.(1963),Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate. Analytical Chemistry35(12), 1950-1953[LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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