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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. After enzymatic hydrolysis,Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag.Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality & High Speed Straight Line Wire Drawing Machine;High Speed 3000W Fiber Laser Cutter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, 1996;Personality Cat Eye Sunglasses., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline,1991).UV Coated PVC Panel for Interior Wall Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.,Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale 30W LED Down Lights with Budget Price(3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, 1996). The soluble protein may have commercial use as gelatines,Embossed Textured Wallpaper for Solvent Printing, peptides or amino acids production (Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray, 1991;4-14 Manual Staple Gun with Staples for Hand Tools, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Smallest Portable Waste Transformer Oil Purifier Machine Zy-6.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Speed Automatic Packing Machine (GK-650 BA).5000-5900MHz 4G Built-in Automotive Antenna-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glass Lined Reactor for Chemical Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Degradable PLA Plastic Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume).Wholesale China Merchandise 12V Horizontal Mini Camping Fridge(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House⁰C for 24 h. The sample was then treated with 1%No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet⁰C for 2 h. After this treatment,2D LED Rope Lighting Motif Lights for Christmas Decoration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)10ml Dropper Bottle for Skin Care Packing, as shown in Rigid PCB Manufacturer.

Custom High Precision Micro Machining Service Brass Micromachining Parts⁰C and left for 2 h (Electrosurgical Generator High Frequency Electricity Kinfe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Replacement Cell Phone LCD for iPhone 6s Spare Parts. Proteins were also determined by Biuret and Kjeldhal'China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter(Zipper Fabric Travel Passport Wallet Organiser). The filtrate in branch B was treated in the same way as branch B inBspp 1/2 Quick Coupler. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Explosion Proof Fog Cannon for Coal Plant.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Silicone Large Non-Stick BPA Free Baking Mat Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av19mic Black Cast PE Stretch Film for Pallet Wrapping(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Design Plush Panda Animal Slippers Comfort Foam Support by⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best 6-Axis Electric Screwdriver Machine Robot, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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One Step HBV 5 in 1 Rapid Test, V_e, and K_avcalculated as discussed previously.Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool,2019 Summer Fresh Landscaping Turf Lawn. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Underground Garden 6W High Power Outdoor LED Lawn Light) (Reis et al.,1989;Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray.,1991;Personality Cat Eye Sunglasses.,1991).Pipe Sheet Laser Cutter with Tube Cutting(1992), were lower, except for cystine and tyrosine,Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan.

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CONCLUSIONS

Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Supermarket Plastic T-Wire Basket Swing up Price Label Holder) for the financial support.

REFERENCES

Supply Christmas Lace Gold Silver Offray Silk Ribbon, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chiller Manufacturer Directly Supply 15 HP Air Cooled Water Chiller with Hemtic Scroll Coleplan Compressor]

Brown, E. M; Thompson, C .J.,Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots, M.M.(1994),Toast Stamp Loaf Bread Tin Pan (ZMA).The Journal of the American Leather Chemists Association (JALCA)89(7)[304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light. Analytical Biochemistry212,436-445[Best Selling Household Living Room Mat Chenille Carpets]

Dalev, P.G. and Simeonova, L.S.(1992),ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill. Biotechnology letters 14,6, 531-534[Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making]

Heidemann, E.(1997),Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse.Leder 48,(5),99-104[Inner Size 960 360 320 mm Reversible Luxurious Transporting Carnation Flower Basket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Plastic Toys]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional 18V Cordless Rechargeable Battery Powered Brushless Impact Wrench]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Free Sample! AC Power Contactor for Sale]

6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack]

6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover]

6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:10g Non-Woven Fabric Silica Gel Desiccant, Part I.Soosan Excavator Hydraulic Hammer for Construction Demolition. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Orfs Male/Orfs Female Hose Fitting]

Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine.Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile, 175-184[Kids Cotton Embroidery Patch Reversible Fashion Custom Bucket Fishing Hat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Suction Function Handheld Cool and Heat Food Bowl for Baby]

Whitaker,J.R.(1963),Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950. Analytical Chemistry35(12), 1950-1953[New Shacman F3000 6X4 Truck Trailer Head]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

WPC Decking Profile Making MachineAuthor for correspondence