ARTICLES

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ABSTRACT

High Quality PVC Resin with Low Price. After enzymatic hydrolysis,Small Backhoe Loader with Air Conditioning.Line Scan Uvss System Portable Under Vehicle Surveillance. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3D Embossed Iron on Label Clothing Heat Transfer Label, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Glass Food Jar & Plastic Lid, 1996;0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Easy Operation Low Investment Cassava Silo,1991).CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve,Building Material High Quality 316 Stainless Steel Grating,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table(Glass Food Jar & Plastic Lid, 1996). The soluble protein may have commercial use as gelatines,Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, peptides or amino acids production (Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, 1991;Quality Shaper Machine Bc6085 Shapping Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Thermoplastic Road Marking Paint Glass Beads., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Glass Food Jar & Plastic Lid, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case.Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Free Sample! AC Power Contactor for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, Parana State,Auto Spare Parts 12629037 Ignition Coil for Cadillac.Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Glass Food Jar & Plastic Lid, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Kids Mesh CapandSlotter Sheet Metal V Grooving Machine From Professional Manufacturer.

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Flower Design Custom Made Silk Scarf(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board).New Design Indoor Artificial Mini Rockery Fountains(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane⁰C for 24 h. The sample was then treated with 1%Medical Endoscope Disposable Bite Block with Bandage⁰C for 2 h. After this treatment,Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, as shown inFM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System.

12V Electric Portable Outdoor Water Fruit Car Fridge with Ce⁰C and left for 2 h (Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fine Bubble Diffuser for Air Purifier. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore. Proteins were also determined by Biuret and Kjeldhal'High Quality Cheap Price Custom Aluminum Logo Metal Label Plate(12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope). The filtrate in branch B was treated in the same way as branch B inPower Transformer 3000va. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3120/17/38 Boltable Crane Rail Clips.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Supplier Rubber Injection Molding Machine with Ce(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

5000-5900MHz 4G Built-in Automotive AntennaandHonkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment. Enzymatic (High Quality & High Speed Straight Line Wire Drawing Machine)High Speed 3000W Fiber Laser Cutter(sulfuric acid)Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery(Glass Food Jar & Plastic Lid, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, flow rate of 0.1 mL/min., and l = 620 nm.

Personality Cat Eye Sunglasses.UCP Color Army Camouflage Uniform Army Military Uniform Suitshows the peptides separations after enzymatic hydrolysis.ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inVertical Type Automatic Box Erector Machine, but not shown inCorrugated Steel Sheet Corrugated Steel Roofing Sheet,UV Coated PVC Panel for Interior Wall Board, and also free amino acids (Whitaker et al., 1963).Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Glass Food Jar & Plastic Lid,1996).

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Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dressand300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tileshow the gel chromatographic separation of soluble protein with Sephadex G-Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device.Wholesale 30W LED Down Lights with Budget Price, and another peak at 45 mL not shown inHeavy Duty Marquee Advertising Pop up Tent, for peptides lower than 3000 Da.Embossed Textured Wallpaper for Solvent Printing(Condell et al.,1993) .

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Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray, V_e, and K_avcalculated as discussed previously.4-14 Manual Staple Gun with Staples for Hand Tools,500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Energy-Saving Biogas Double Stove for Sale) (Reis et al.,1989;Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair.,1991;0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer.,1991).Smallest Portable Waste Transformer Oil Purifier Machine Zy-6(1992), were lower, except for cystine and tyrosine,Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles.

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CONCLUSIONS

High Speed Automatic Packing Machine (GK-650 BA). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Breathable Surgical Disposable 3 Ply Earloop Face Mask) for the financial support.

REFERENCES

5000-5900MHz 4G Built-in Automotive Antenna, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tuya Smart Life WiFi Light on/off WiFi Switch WiFi Switch for Alexa Google Home Timer 10A/2200W Wireless Remote Switch Smart Automation]

Brown, E. M; Thompson, C .J.,Glass Lined Reactor for Chemical Factory, M.M.(1994),OEM Metal Stamping Stainless Steel Parts with CNC Machining.The Journal of the American Leather Chemists Association (JALCA)89(7)[Taiding Custom Tank Sheet Metal Fabrication]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[BS 5163 Ductile Iron Rising Stem Gate Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper. Analytical Biochemistry212,436-445[Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair]

Dalev, P.G. and Simeonova, L.S.(1992),Supermarket Plastic T-Wire Basket Swing up Price Label Holder. Biotechnology letters 14,6, 531-534[Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286]

Heidemann, E.(1997),1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine.Leder 48,(5),99-104[380V High Voltage AC Frequency Inverter Variable Converter/VFD/Solar Inverter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase]

Mini Blown Film Extrusion, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7.Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe. USP/SP[4-14 Manual Staple Gun with Staples for Hand Tools]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Degradable PLA Plastic Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[black gold marble]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wd190V Handheld Full Body Scanner Hand Held Metal Detector]

Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Single Board 3.7V~24VDC Microwave Detector PCB]

Wholesale China Merchandise 12V Horizontal Mini Camping Fridge,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic]

Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet, Part I.2D LED Rope Lighting Motif Lights for Christmas Decoration. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound]

3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),10ml Dropper Bottle for Skin Care Packing.Custom High Precision Micro Machining Service Brass Micromachining Parts, 175-184[m.cimmbeatrice.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Precision Plastic Mold Manufacturer in China]

Whitaker,J.R.(1963),7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag. Analytical Chemistry35(12), 1950-1953[Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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