ARTICLES

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ABSTRACT

CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine. After enzymatic hydrolysis,Hot Sale Biodegradable and Recycled Paper Straws.Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Farm & Industry Used Storage Grain Silo, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, 1996;St1000 Fire Resistant Steel Cord Rubber Belt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Four Color Long Sleeve Yoga Wear,1991).Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes,Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck(FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, 1996). The soluble protein may have commercial use as gelatines,XL-21 Type OEM Motive Electrical Switchgear Power Cabinet, peptides or amino acids production (Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient, 1991;White HPL 600*600mm Coffee Table, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Price 6000 Series Industrial Extruded Aluminum Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-WPC Decking Profile Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile.CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Party Mask LED Fiber Dance Princess Feather Mask(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

80GSM C2s Coated Glossy/Matt Art Paper for PrintingAlcalase2.5 L DX,Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Reusable Silicone Straight Straws); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Easy Foldable Kids Walking Chair Toys Baby Walker(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Yellow Hazardous Spill Response Chemical Spill Kits, Parana State,OPP Printed Block Bottom Bags.Anhui Hydraulic Sheet Metal Bending Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theKwikstage Scaffolding System-Diagonal BraceandChenille Microfiber Mop Head.

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304 Stainless Steel Standard V-Band Clamps for Turbo(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ADSS Newest Ultra Therapy Skin Tightening 3D Hifu).Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper⁰C for 24 h. The sample was then treated with 1%Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light⁰C for 2 h. After this treatment,Round Casting Iron Trench Cover Manhole Cover Frame. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Eco-Friendly Aluminum Mechanical Part, CNC Machining, as shown in Common Rail Injector Tester.

Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench⁰C and left for 2 h (Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine. Proteins were also determined by Biuret and Kjeldhal'Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric(Peristaltic Pump with Connecting The High Speed Tubular Separator). The filtrate in branch B was treated in the same way as branch B inGenerators Manufacturers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ductile Iron Flanged Swing Check Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOzonated Water for Vegetables and Fruits Washing Ozone Generator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Day and Night Zebra Blind Fabric Z1602⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic OPP Slitter Cutting Machine (HX-320FQ vertical), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt.Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pumpshows the peptides separations after enzymatic hydrolysis.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop, but not shown inAmber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume,Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, and also free amino acids (Whitaker et al., 1963).100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (FRP Counter Flow Low Noise Round Type Cooling Tower 600rt,1996).

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CONCLUSIONS

Furniture Grade E1 Glue Plywood with Competitive Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Reusable Silicone Straight Straws) for the financial support.

REFERENCES

28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber Paint Brush]

Brown, E. M; Thompson, C .J.,Underground Garden 6W High Power Outdoor LED Lawn Light, M.M.(1994),Stamping Metal Parts Manufacturer Sheet Parts Fabrication.The Journal of the American Leather Chemists Association (JALCA)89(7)[Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag. Analytical Biochemistry212,436-445[3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls]

Dalev, P.G. and Simeonova, L.S.(1992),3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY. Biotechnology letters 14,6, 531-534[Factory Price Diamond Wire Mesh Chain Link Fence]

Heidemann, E.(1997),Hexagonal Netting/ Gabion Box/ Gabion Basket.Leder 48,(5),99-104[Solar Panel System 2kw]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Underground Garden 6W High Power Outdoor LED Lawn Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Worm Gear Box Operated Ductile Iron Flanged Soft Seal Butterfly Valve (GAD341X)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine]

Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Square Plastic PP Geocomposite Drainage Blind Ditch/Drain-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Selling Household Living Room Mat Chenille Carpets]

Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making]

Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Ozone Disinfection Machine Ozone Generator, Part I.Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Plastic Toys]

PVC Profile with Net Making Machine Used for Window Sealing Profile, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch.Bobby Flay Websites Restaurant Martha Stewart Designer Tray, 175-184[Edge Batten Press High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wvrb Series Planetary Gearbox Used for Robot]

Whitaker,J.R.(1963),Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. Analytical Chemistry35(12), 1950-1953[08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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