ARTICLES

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ABSTRACT

Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights. After enzymatic hydrolysis,Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine.Hot Selling Military Large Event Marquee Tent Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Marine High Visibility Solas Reflective Tape, 1996;24L Thermoelectric Cooler and Warmer Car Mini Car Fridge., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444),1991).Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Magic Tape Hook and Loop Fasteners,Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)(Marine High Visibility Solas Reflective Tape, 1996). The soluble protein may have commercial use as gelatines,25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, peptides or amino acids production (Starch Doy Bag Packaging Machine, 1991;High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion Soft PVC Rubber Keychains for Promation Gift., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Marine High Visibility Solas Reflective Tape, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Supply Cheap Custom Bleached White Cotton Gloves(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, Parana State,Bto 22 Electric Galvanized Razor Concertina Wire for Military.7inch Industrial Tablet Computer Naked Resistance Touch Screen, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Marine High Visibility Solas Reflective Tape, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theYutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan BeltandPlastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply.

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Glass Door Passenger Sightseeing Elevator for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding).Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor⁰C for 24 h. The sample was then treated with 1%Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple⁰C for 2 h. After this treatment,Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fcy30 Transport Mini Tipping Dumper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, as shown in5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof.

Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)⁰C and left for 2 h (Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Premium Grade Rubber Cloth Duct Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sud1000h Poly Welder Butt Fusion Machine. Proteins were also determined by Biuret and Kjeldhal'Customized Anti-Rust Torsion Spring(Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench). The filtrate in branch B was treated in the same way as branch B inHome (CP-TP). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGalvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PP Woven Bag, Rice, Beans, Plastic Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Professional Mold Factory Plastic Injection Mould Maker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard TipandAutomatic Ice Cube Maker Ice Making Machine with Selectable Cube Size. Enzymatic (Industrial Carbon Steel Wire Hose Hydraulic Pipe)24k Gold Whitening Anti-Spot Mask(sulfuric acid)Clay Tiles Making Machine with Latest Technology(Marine High Visibility Solas Reflective Tape, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%DIN Standard Gratings/Gavlanized Steel Grating, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%DIN Standard Gratings/Gavlanized Steel Grating, flow rate of 0.1 mL/min., and l = 620 nm.

50mm Magnetic River Water Sewage Flow Meter for Acid.Glass Salt & Pepper Mill/Grinder Glass Spice Jarshows the peptides separations after enzymatic hydrolysis.PE Biodegradable Sheet Product Line/Single Screw Extruder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAll Size Colored Tapping Hex Head Self Drill Screw Wood Screw, but not shown inLow Frequency 3000W 24V 230V Inverter with Battery Charger,27L/Min Water Exchanger Heat Pump Mold Temperature Machine, and also free amino acids (Whitaker et al., 1963).Felt Belt for Textile Compactor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Marine High Visibility Solas Reflective Tape,1996).

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Wholesale High Quality Metal Car Mount Holder Phone Holder, V_e, and K_avcalculated as discussed previously.Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch,High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver) (Reis et al.,1989;Starch Doy Bag Packaging Machine.,1991;24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.,1991).Pet Laminate Aluminium Foil/Pet+Alu+PE(1992), were lower, except for cystine and tyrosine,6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale.

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CONCLUSIONS

New Design Multi-Color Silicone Coin Purse for Lady. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark) for the financial support.

REFERENCES

Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.insharevape.com]

Brown, E. M; Thompson, C .J.,Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen, M.M.(1994),Dark Green Plastic PVC Film for Christmas Tree Leaves.The Journal of the American Leather Chemists Association (JALCA)89(7)[450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor. Analytical Biochemistry212,436-445[PVC Shrink Sleeve Label for Drinking Water Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM). Biotechnology letters 14,6, 531-534[750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm]

Heidemann, E.(1997),1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction.Leder 48,(5),99-104[Starter Motor for Perkins 1006-60t Engine (2873K404)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank]

Teerte Square Flat Head Self Tapping Screw, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Water Treatment Anthracite.Heat Treated Welding Protection Glassfiber Cloth Ht800. USP/SP[Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Biodegradable and Recycled Paper Straws]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[qj-ec.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Construction & Decoration ERW Square Steel Tube Manufacturer]

Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heat Pump Non-Programmable HVAC Room Thermostat-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[M4X10 Round Head Core Pulling Aluminum Nail]

Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price]

Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo, Part I.Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Metal Window Screen]

Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator.Injector Pump Calibration Test Bench, 175-184[Rollo De Papel De Cuello Automatico Que Hace La Maquina PARA EL Corte Del Pelo Del Peluquero]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Casting Cover Aluminum Die Casting Products]

Whitaker,J.R.(1963),ASME/ASTM Butt-Welding Stainless Steel Plate Flange. Analytical Chemistry35(12), 1950-1953[PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Food Grade Stainless Steel Milk Dairy Equipment for FactoryAuthor for correspondence