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ABSTRACT

Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. After enzymatic hydrolysis,Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration.Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Ozone Disinfection Machine Ozone Generator;Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PVC Profile with Net Making Machine Used for Window Sealing Profile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, 1996;Bobby Flay Websites Restaurant Martha Stewart Designer Tray., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line,1991).Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Diameter 2.68m Paragraphed Autoclave for AAC Bricks,Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Travelling Weekender Mens Canvas Duffle Gym Sports Bag(Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, 1996). The soluble protein may have commercial use as gelatines,4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box, peptides or amino acids production (China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), 1991;Best Selling Household Living Room Mat Chenille Carpets, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;130HP 4WD Large Farm Power Engine Tractor From High Quality Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Large Size Outdoor Store Front Signs Advertising Billboard. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg.1325 CNC Engraving Router-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Round Shape All Color Pets Feeding Dog Bowl of China Supplies, Parana State,Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla.Wvrb Series Planetary Gearbox Used for Robot, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theViton O Ring Kit with Excellent with Oil ResistanceandMetal Steel Frame Membrane Structure Car Parking Shed Cover Design.

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NPK 15 15 15 Compound Fertilizer at a Hot Selling(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Quality Zinc Plated Slotted Hex Head Roofing Screw).Mini Blown Film Extrusion(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pearls Flake 99% Factory Naoh Caustic Soda⁰C for 24 h. The sample was then treated with 1%Auto Spare Parts 12629037 Ignition Coil for Cadillac⁰C for 2 h. After this treatment,High Quality PVC Resin with Low Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small Backhoe Loader with Air Conditioning's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Line Scan Uvss System Portable Under Vehicle Surveillance, as shown inRailway Freight Wagon Spare Parts Casting and Forging for Bogie.

Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light⁰C and left for 2 h (Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.FSTN Type Monochrome Graphic Display Cog LCD with Backlight. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3D Embossed Iron on Label Clothing Heat Transfer Label. Proteins were also determined by Biuret and Kjeldhal'Glass Food Jar & Plastic Lid(10g Non-Woven Fabric Silica Gel Desiccant). The filtrate in branch B was treated in the same way as branch B in2 in 1 Skin Care Beauty Equipment Multipolar RF Radio Frequency Machine for Anti Wrinkle RF3.0. The final soluble protein shown were used for peptides separations using gel permeation chromatography,0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Easy Operation Low Investment Cassava Silo. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Building Material High Quality 316 Stainless Steel Grating⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic Injection for OEM ProductandAmazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. Enzymatic (Quality Shaper Machine Bc6085 Shapping Machine)Thermoplastic Road Marking Paint Glass Beads(sulfuric acid)Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine(Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Medical Endoscope Disposable Bite Block with Bandage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Machining 6601 Aluminum Parts with Anodized Automoblile) for the financial support.

REFERENCES

Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[ASTM A536 Material Hose Clamps with UL FM Approval]

Brown, E. M; Thompson, C .J.,Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), M.M.(1994),Ladder Type Cable Tray with Ce/ GOST/ TUV/UL.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. Analytical Biochemistry212,436-445[Children Bear English Story Book Printing]

Dalev, P.G. and Simeonova, L.S.(1992),Fine Bubble Diffuser for Air Purifier. Biotechnology letters 14,6, 531-534[ KTV]

Heidemann, E.(1997),Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore.Leder 48,(5),99-104[18K Yellow Gold Custom Old Mine Cut Moissanite Diamond Engagement Rings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Black Oiled Carbon Steel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector]

China Supplier Rubber Injection Molding Machine with Ce, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo]

China Supplier Rubber Injection Molding Machine with Ce,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Felt Belt for Textile Compactor]

China Supplier Rubber Injection Molding Machine with Ce, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, Part I.High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve]

Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment.High Quality & High Speed Straight Line Wire Drawing Machine, 175-184[Ahu With Condensing Unit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery]

Whitaker,J.R.(1963),High Speed 3000W Fiber Laser Cutter. Analytical Chemistry35(12), 1950-1953[Wall Washer Light Building Decoration Landscape LED IP65 Linear Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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