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ABSTRACT

Acm Dewatering Twin Screw Extruder. After enzymatic hydrolysis,Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820).Tumble Belt Type Shot Blasting Machine for Iron Castings Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pet Hair Remover Glove for Dog, Grooming Gloves Brush, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, 1996;Alumina Balls for Hydogen Peroxide., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anti-High Pressure Insulated Pliers with PVC Dipped Handles,1991).Hot Selling Electronical Blue Pet Plastic Release Film Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DIN127 Stainless Steel Spring Lock Washer,29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Condition Hot Sale Automatic Animal Feed Cooler Machine(Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, 1996). The soluble protein may have commercial use as gelatines,Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, peptides or amino acids production (Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), 1991;Garlic Red Mesh Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ground Finish Molybdenum Tzm Rod.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Transparent/Clear BOPP Jumbo Roll. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Yashi 19 270degree Pyramid Hologram Display Showcase Attractive.Chenille Microfiber Mop Head-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Waterproof Pizza Sign Large Advertising Signboard(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Children Bear English Story Book Printing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices).Flexible Board FPC PCB Camera Module Rigid Flex PCB(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing⁰C for 24 h. The sample was then treated with 1%Cherry Wooden Grain PVC Film in Furniture⁰C for 2 h. After this treatment,Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), as shown inCNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine.

Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump⁰C and left for 2 h (Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Linen Quiltcover Set with Quilting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life. Proteins were also determined by Biuret and Kjeldhal'Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace(Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch). The filtrate in branch B was treated in the same way as branch B in4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Teerte Square Flat Head Self Tapping Screw.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBto 22 Electric Galvanized Razor Concertina Wire for Military(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Selling Military Large Event Marquee Tent Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High-Quality Pre-Seasoned Cast Iron Wok, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Factory Supply Cheap Custom Bleached White Cotton Gloves. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Galvanized Square Wire Mesh, Metal Window Screen) for the financial support.

REFERENCES

2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PTFE Oil Seal / Teflon Oil Seal]

Brown, E. M; Thompson, C .J.,304 &314 Stainless Steel Seamless Bending Tube, M.M.(1994),Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark.The Journal of the American Leather Chemists Association (JALCA)89(7)[Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Grey Full-Zip Cotton Mens Hoodies. Analytical Biochemistry212,436-445[Fire Extinguisher UV Curing Machine Cylinder Drying Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. Biotechnology letters 14,6, 531-534[700mm RP HP UHP High Quality Graphite Electrode]

Heidemann, E.(1997),7inch Industrial Tablet Computer Naked Resistance Touch Screen.Leder 48,(5),99-104[Showcomplex pH2.5 Indoor LED Wall Mounted Display Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Betaine 98% Betaina Feed Grade]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vertical Automatic Granule Bag Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cast Steel Gate Valve Class 150/300/600]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal]

Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa]

Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Damask Pillows Cover]

Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fcy30 Transport Mini Tipping Dumper, Part I.Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hf Vacuum Dryer Machine with Advanced High Frequency Technology]

Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Premium Grade Rubber Cloth Duct Tape.Sud1000h Poly Welder Butt Fusion Machine, 175-184[Ride On Floor Cleaning Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction]

Whitaker,J.R.(1963),Customized Anti-Rust Torsion Spring. Analytical Chemistry35(12), 1950-1953[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Polished Tzm Molybdenum Alloy PlateAuthor for correspondence