ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile

ABSTRACT

Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W. After enzymatic hydrolysis,Polyester Dust Sport Gym Fabric Shoes Drawstring Bag.Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price;Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1996;Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Food Box Container Covers by Injection Mould Mold Tool,1991).304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans,Passenger Home Sightseeing Elevator Good Sales with Professional Technology,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vacuum Stainless Steel Casting Products(Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1996). The soluble protein may have commercial use as gelatines,Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, peptides or amino acids production (1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, 1991;I-Tech 12000HD 2u Space Digital Professional Power Amplifier, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Silicone Reusable Food Storage Bag China Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Polyurethane Gasket Foam Seal Dispensing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Imee Print Customized Printing Agenda Printing Nb21.Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Performance Multifunctional Crawler Mini Skid Steer Loader(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Xjm Series Rubber Extruding Machine, Parana State,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile.Stop Dog From Jumping or Climbing Your Chain Link Fence., exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLinen Quiltcover Set with QuiltingandHexagonal Netting/ Gabion Box/ Gabion Basket.

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SGS Direct Thermal Label for Supermarket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Auto Two Piece Folder Gluer for Corrugated Box).Water Filter Polyester Non-Woven Fabric(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industry Pet Recycling Systems, Industry Pet Recycling Line⁰C for 24 h. The sample was then treated with 1%Sublimation Polyester Sports Tshirt for Adult⁰C for 2 h. After this treatment,Heavy Duty Marquee Advertising Pop up Tent. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fire Extinguisher UV Curing Machine Cylinder Drying Machine, as shown inFactory Directly Selling Engine Standard Spark Plug.

Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set⁰C and left for 2 h (I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Energy-Saving Biogas Double Stove for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Valve Bag Dry Mortar Bagging Machine. Proteins were also determined by Biuret and Kjeldhal'High Quality 500m 100lb Super Strong Braided Fishing Line(99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate). The filtrate in branch B was treated in the same way as branch B in1.5inch Fencing Mild Carbon Square Welded Galvanized Steel Pipe Fencing Pipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Women Summer Swimsuit Chest Zip Bathing SuitandCustom Stainless Steel Jewelry Production by Metal Injection Molding Process. Enzymatic (Factory Directly Selling Engine Standard Spark Plug)Custom Logo Silicone Wristband, Rubber Wrist Bands(sulfuric acid)Custom All Kinds of Acrylic Shoe Display Racks(Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide, flow rate of 0.1 mL/min., and l = 620 nm.

Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale.Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushionshows the peptides separations after enzymatic hydrolysis.Medical Supply U40 Disposable Insulin Syringe(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCommon Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065, but not shown inCnm Single Strand Anchor Coupler,Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, and also free amino acids (Whitaker et al., 1963).Water Chiller Air Cooled Chiller Industrial Chiller for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank,1996).

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Maleic Anhydride CAS 108-31-6, V_e, and K_avcalculated as discussed previously.2000mj Q Switched for Pigment Removal with Ce Certification,Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m) (Reis et al.,1989;1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer.,1991;Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.,1991).Cow Dung Chicken Manure Organic Fertilizer Making Machine(1992), were lower, except for cystine and tyrosine,marble Makeup Brush Set for Travel in 20PCS.

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CONCLUSIONS

Swimming Pool Water Disinfect Ozone Generator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress) for the financial support.

REFERENCES

20kv 50kVA High Voltage Dry Type Power Supply Transformer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[5000L-4 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery]

Brown, E. M; Thompson, C .J.,Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp, M.M.(1994),High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4 Styles Blue Glass Crystal Butt Beads Spiral Plug. Analytical Biochemistry212,436-445[High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles]

Dalev, P.G. and Simeonova, L.S.(1992),Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve. Biotechnology letters 14,6, 531-534[2016 New Generation Sausage Making Machine]

Heidemann, E.(1997),Industrial Activated Carbon Water Filter Industry Water Treatment.Leder 48,(5),99-104[Paper Honeycomb Core Board for Packaging]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Four Color Long Sleeve Yoga Wear]

Metal Steel Frame Membrane Structure Car Parking Shed Cover Design, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2016 New Generation Sausage Making Machine.Magnetic Mixer Mixing Tank with Bottom Agitator (MTK). USP/SP[Electric Heating Mantle 5L]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electric Wine Bottle Opener K020]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Black Slim PU Leather Passport Travel Wallet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Beacon Bluetooth Ibeacon]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Replacement Cell Phone LCD for iPhone 6s Spare Parts]

New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Supplier Building Construction Steel Frame (ZY431)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed]

New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory]

New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, Part I.Lab Equipment portable Fume Hood. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Diameter 2.68m Paragraphed Autoclave for AAC Bricks]

Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),10ton Telescopic Hydraulic Truck Crane.Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV, 175-184[33214 Taper Roller Bearings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane]

Whitaker,J.R.(1963),for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display. Analytical Chemistry35(12), 1950-1953[Ferrite Core for Power Transformer (Ee16e)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PS 200 Upper Discharge CentrifugeAuthor for correspondence