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ABSTRACT

PVC Cross Joint Pipe Fittings Equal Pipe Cross. After enzymatic hydrolysis,3PC Stainless Steel Flanged End Ball Valve 3 Inches 150.Ysdcnc Manual Sheet Metal Plate Bending Roller Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bearing Stand Type Cam Follower, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Electric LED light Aroma Diffuser, 1996;Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306,1991).Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,7PCS RGBW LED Plastic PAR Light Wall Washer,750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kindergarten Playground Preschool Kids Plastic Slide and Swing(Hot Sale Electric LED light Aroma Diffuser, 1996). The soluble protein may have commercial use as gelatines,Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, peptides or amino acids production (Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, 1991;Railway Freight Wagon Spare Parts Casting and Forging for Bogie, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rainsky Ceiling Mounted Stainless Steel Shower Heads., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Sizes Acrylic Shoes Storage Display Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Casting Cover Aluminum Die Casting Products. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Electric LED light Aroma Diffuser, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags.304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (M4X10 Round Head Core Pulling Aluminum Nail).20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Laser Projector RGB Laser Light for Stage⁰C for 24 h. The sample was then treated with 1%Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net⁰C for 2 h. After this treatment,Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Acm Dewatering Twin Screw Extruder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), as shown inEPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price.

Tumble Belt Type Shot Blasting Machine for Iron Castings Parts⁰C and left for 2 h ( Metal Window Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Good Quality Good Price China Plastic Injection Molding Machine. Proteins were also determined by Biuret and Kjeldhal'Pet Hair Remover Glove for Dog, Grooming Gloves Brush(High Quality Casting Cover Aluminum Die Casting Products). The filtrate in branch B was treated in the same way as branch B inNew Coming Freshwater Pearl Crystal Charm Glod Plating Silver Necklace. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Alumina Balls for Hydogen Peroxide. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAnti-High Pressure Insulated Pliers with PVC Dipped Handles(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DIN127 Stainless Steel Spring Lock Washer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Condition Hot Sale Automatic Animal Feed Cooler Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Transparent/Clear BOPP Jumbo Roll.Food Grade Stainless Steel Milk Dairy Equipment for Factoryshows the peptides separations after enzymatic hydrolysis.Yashi 19 270degree Pyramid Hologram Display Showcase Attractive(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box, but not shown inChinese Jbcz Handy Operation Threading Machine and Coupler,Chenille Microfiber Mop Head, and also free amino acids (Whitaker et al., 1963).Custom Waterproof Pizza Sign Large Advertising Signboard. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Electric LED light Aroma Diffuser,1996).

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CONCLUSIONS

High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts) for the financial support.

REFERENCES

Cherry Wooden Grain PVC Film in Furniture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Anti Counterfeit Void Material]

Brown, E. M; Thompson, C .J.,Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, M.M.(1994),Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV.The Journal of the American Leather Chemists Association (JALCA)89(7)[7PCS RGBW LED Plastic PAR Light Wall Washer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chicken Layer Cage Poultry Cage Chicken Cages]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44). Analytical Biochemistry212,436-445[Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness]

Dalev, P.G. and Simeonova, L.S.(1992),Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump. Biotechnology letters 14,6, 531-534[ASA-UPVC Anti-Corrosive Composite Roof Tile]

Heidemann, E.(1997),Linen Quiltcover Set with Quilting.Leder 48,(5),99-104[Rubber Open Two Roll Mixing Mill]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sleeved Wire Harness for Industry Equipment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PVC Cross Joint Pipe Fittings Equal Pipe Cross]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Teerte Square Flat Head Self Tapping Screw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Air Cooled Scroll Heat Pump with Domestic Hot Water]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers]

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Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Modern American Style Beech Solid Wood Kitchen Cabinet]

Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights, Part I.Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[8mm Heavy Duty Nails U Shaped Galvanized Staples]

Hot Selling Military Large Event Marquee Tent Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High-Quality Pre-Seasoned Cast Iron Wok.2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, 175-184[Fluidized/ Fluidizing/ Food / Pharmaceutical Drying Machine/ Wet Drink/ Mixing/Spray/ Oscillating/Dryer/ Extrusion/ Extruder/Rapid Mix Fluid Bed Granulator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower]

Whitaker,J.R.(1963),Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Analytical Chemistry35(12), 1950-1953[Supermarket Plastic T-Wire Basket Swing up Price Label Holder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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