ARTICLES

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ABSTRACT

Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. After enzymatic hydrolysis,7inch Industrial Tablet Computer Naked Resistance Touch Screen.Glass Door Passenger Sightseeing Elevator for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, 1996;Fcy30 Transport Mini Tipping Dumper., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park,1991).Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Premium Grade Rubber Cloth Duct Tape,Sud1000h Poly Welder Butt Fusion Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Anti-Rust Torsion Spring(Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, 1996). The soluble protein may have commercial use as gelatines,Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining, peptides or amino acids production (New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, 1991;Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PP Woven Bag, Rice, Beans, Plastic Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Professional Mold Factory Plastic Injection Mould Maker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated.Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Carbon Steel Wire Hose Hydraulic Pipe(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PE Biodegradable Sheet Product Line/Single Screw Extruder, Parana State,Custom Grey Full-Zip Cotton Mens Hoodies.27L/Min Water Exchanger Heat Pump Mold Temperature Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh-Efficient Htk Walnut Shell Filter for Water PurificationandPrepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.

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Felt Belt for Textile Compactor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Quality PVC Plastic Ceiling for Wall Panel).Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Metal Soft Enamel Souvenir Metal Medal Sport Medallions⁰C for 24 h. The sample was then treated with 1%20L Water Tank Lid Mold 3D Printing Samples Available⁰C for 2 h. After this treatment,Wholesale High Quality Metal Car Mount Holder Phone Holder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, as shown inY2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor.

Pet Laminate Aluminium Foil/Pet+Alu+PE⁰C and left for 2 h (Premium Grade Rubber Cloth Duct Tape). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Design Multi-Color Silicone Coin Purse for Lady. Proteins were also determined by Biuret and Kjeldhal'Common Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065(Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)). The filtrate in branch B was treated in the same way as branch B inFiber Optic Cable Amplifier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Portable Folding Table for Camping, Picnic, Outdoor, Family Party Form Camping Kitchen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dark Green Plastic PVC Film for Christmas Tree Leaves. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDesktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Water Treatment Anthracite, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heat Treated Welding Protection Glassfiber Cloth Ht800, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical NodeandFashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. Enzymatic (Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector)Heat Pump Non-Programmable HVAC Room Thermostat(sulfuric acid)Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo(Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable, flow rate of 0.1 mL/min., and l = 620 nm.

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Granite Stone Jaw Crusher of Mining Machine, V_e, and K_avcalculated as discussed previously.Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor,Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Design Multi-Color Silicone Coin Purse for Lady) (Reis et al.,1989;New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.,1991;Fcy30 Transport Mini Tipping Dumper.,1991).The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine(1992), were lower, except for cystine and tyrosine,Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag.

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CONCLUSIONS

Wholesale All Kinds Cheap Plush Animal Baby Slippers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DIN Standard Gratings/Gavlanized Steel Grating) for the financial support.

REFERENCES

Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Diy Storage Cube Cabinet Wardrobe Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, M.M.(1994),Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dolphin Designed Handheld Massager (LC-889)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Prices of 20W 40W 60W Solar Street Lights]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress. Analytical Biochemistry212,436-445[ Kids Mesh Cap]

Dalev, P.G. and Simeonova, L.S.(1992),Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale. Biotechnology letters 14,6, 531-534[Slotter Sheet Metal V Grooving Machine From Professional Manufacturer]

Heidemann, E.(1997),Taiding Custom Tank Sheet Metal Fabrication.Leder 48,(5),99-104[Flat Panel Tv Wall Brakcet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Spare Parts For Mg]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope]

3 Layer Round Tea Tin with Air Tight Lid, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China PA System Power Amplifier with Enough Rated Power-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5000-5900MHz 4G Built-in Automotive Antenna]

3 Layer Round Tea Tin with Air Tight Lid,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[UCP Color Army Camouflage Uniform Army Military Uniform Suit]

3 Layer Round Tea Tin with Air Tight Lid, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Export Grade Citric Acid Monohydrate as Detergent, Part I.Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vertical Type Automatic Box Erector Machine]

Familu Use Mini Small Pig Feed Pellet Mill, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light.Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer, 175-184[4*8 Bintangor Commercial Plywood]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Corrugated Steel Sheet Corrugated Steel Roofing Sheet]

Whitaker,J.R.(1963),Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed. Analytical Chemistry35(12), 1950-1953[ Caulk Strip Trim Tape (38mm)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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