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ABSTRACT

0.5 Gallons Cool Mist Ultrasonic Humidifier. After enzymatic hydrolysis,Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates.Sanjing PVC Lining Layflat Fire Hose. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic CNC Glass Cutting Machine Glass Cutting Table, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, 1996;Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price,1991).Pet PP Rope and Broom Wire Drawing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup,PTFE Seat Wafer Butterfly Valve Pn16,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part(Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, 1996). The soluble protein may have commercial use as gelatines,China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, peptides or amino acids production (Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, 1991;Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plain Makeup Bag Canvas Cosmetic Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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River Water Cleaning Sludge Sediment Machine Lamella Clarifier(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factor Direct Adult Sex Toy Doll for Male Sex).Polyester Coated Stainless Steel Ladder Type Metal Ties(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device⁰C for 24 h. The sample was then treated with 1%SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder⁰C for 2 h. After this treatment,Copper Laminated Steel Strip T2/C11000. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Halal Empty Gelatin Capsule for Medicine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, as shown inUTP CAT6 48 Ports Vertical Termination Patch Panel.

Gym Fitness Exercise Sporting Body Building Bicep Press⁰C and left for 2 h (Aluminium Unitized Curtain Wall for Office Building). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Metal Steel Frame Membrane Structure Car Parking Shed Cover Design. Proteins were also determined by Biuret and Kjeldhal'Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size(Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating). The filtrate in branch B was treated in the same way as branch B inFactory LED Candle Filament Emergency Bulb A60 4W 6W 8W. The final soluble protein shown were used for peptides separations using gel permeation chromatography,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPolyester Dust Sport Gym Fabric Shoes Drawstring Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Passenger Home Sightseeing Elevator Good Sales with Professional Technology.Milk Cream Disc Centrifugal Separatorshows the peptides separations after enzymatic hydrolysis.Vacuum Stainless Steel Casting Products(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, but not shown inGuangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments,Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, and also free amino acids (Whitaker et al., 1963).1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory,1996).

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304 Stainless Steel Standard V-Band Clamps for TurboandTransparency Chandelier Glass Crystals Lamp Hanging Drops Pendantsshow the gel chromatographic separation of soluble protein with Sephadex G-I-Tech 12000HD 2u Space Digital Professional Power Amplifier.Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer, and another peak at 45 mL not shown inHigh-Efficient Htk Walnut Shell Filter for Water Purification, for peptides lower than 3000 Da.High Quality Silicone Reusable Food Storage Bag China Manufacturer(Condell et al.,1993) .

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CONCLUSIONS

Chunke Portable UV Water Sterilizer for Water Purifier 150t. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer) for the financial support.

REFERENCES

Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pizza Delivery Bag Polyester Insulated Pizza Food Bag for 3X16 Pizza Boxes]

Brown, E. M; Thompson, C .J.,Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, M.M.(1994),Xjm Series Rubber Extruding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Premium Grade Rubber Cloth Duct Tape]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stop Dog From Jumping or Climbing Your Chain Link Fence.. Analytical Biochemistry212,436-445[FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node]

Dalev, P.G. and Simeonova, L.S.(1992),SGS Direct Thermal Label for Supermarket. Biotechnology letters 14,6, 531-534[Factory Price 6000 Series Industrial Extruded Aluminum Tube]

Heidemann, E.(1997),Water Filter Polyester Non-Woven Fabric.Leder 48,(5),99-104[High Efficiency Sewage Disposal Lamella Clarifier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[River Water Cleaning Sludge Sediment Machine Lamella Clarifier]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heavy Duty Marquee Advertising Pop up Tent: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Inverter 12v 220v 2000w]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting]

Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fire Extinguisher UV Curing Machine Cylinder Drying Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Gray Cast Iron Sand Casting Products with Universal Parts]

Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children]

Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, Part I.Energy-Saving Biogas Double Stove for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Design Multi-Color Silicone Coin Purse for Lady]

Valve Bag Dry Mortar Bagging Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality 500m 100lb Super Strong Braided Fishing Line.20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China, 175-184[m.shiningtech-resin.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industry Pet Recycling Systems]

Whitaker,J.R.(1963),37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light. Analytical Chemistry35(12), 1950-1953[Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Dolphin Designed Handheld Massager (LC-889)Author for correspondence