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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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ABSTRACT

Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets. After enzymatic hydrolysis,OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner.160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Nbm High Quality Auto Part Factory Price 6pk2310, 1996;Ultrasonic Automatic Face Mask Blank Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manufacturer of Soda Ash Dense 99 2% Price,1991).Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Headband Big Headphone EVA Bag Tool Case Specialized Storage Box,Chicken Slaughtering Machine for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish(Nbm High Quality Auto Part Factory Price 6pk2310, 1996). The soluble protein may have commercial use as gelatines,Hf Vacuum Dryer Machine with Advanced High Frequency Technology, peptides or amino acids production (Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers, 1991;Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Nbm High Quality Auto Part Factory Price 6pk2310, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Oak Laminate Grey Floating Cork Vinyl Plank Flooring.China Manufacturer Customized Microwave Ring Ferrite Magnet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Rainsky Ceiling Mounted Stainless Steel Shower Heads).Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sequins Butterfly Handmade Beads Embroidery Patch0C for 24 h. The sample was then treated with 1%Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills0C for 2 h. After this treatment,Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Adjustable Aluminum Solar Mounting System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Wireless Cheapest Android 2D Barcode Scanner, as shown inLarge Variety Low Volume Non Standard Customized High Precision CNC Machining Parts.

Factory Price Diamond Wire Mesh Chain Link Fence0C and left for 2 h (2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Heating Mantle 5L. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration. Proteins were also determined by Biuret and Kjeldhal'99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate(DIN929 Low-Cost Supply Hexagon Weld Nut). The filtrate in branch B was treated in the same way as branch B inWaterproof Human Body Mercury-Free Glass Thermometers Ce Approved. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavBest Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap, pH 3.25., mobile phase B: buffer solution of 0.01 mol/48V DC Powered Air Conditioner for Outdoor Cabinet 2000W, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Galfan Wire (Aluminium-Zinc Alloy coated). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electrical 4W AC Synchronous Gear Motor for Engraving Machine) for the financial support.

REFERENCES

1000base Single Mode 1310nm LC SFP Module 20km 1.25g, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Deep Groove Ball Bearing of 6208 for Construction Machinery]

Brown, E. M; Thompson, C .J.,Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant), M.M.(1994),Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection.The Journal of the American Leather Chemists Association (JALCA)89(7)[Low Power Consumption Laser Foam Vertical Die Cutting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Six Stations Pen Press Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV. Analytical Biochemistry212,436-445[Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fire Extinguisher UV Curing Machine Cylinder Drying Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.usbcmaster.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[700mm RP HP UHP High Quality Graphite Electrode]

Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wall Washer Light Building Decoration Landscape LED IP65 Linear Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Betaine 98% Betaina Feed Grade]

Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine]

Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Stainless Steel Ethanol Processing Plant From Cassava, Part I.5W Ultra-Thin Tiltable LED Down Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vertical Automatic Granule Bag Packing Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

Whitaker,J.R.(1963),Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children. Analytical Chemistry35(12), 1950-1953[Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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