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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles. After enzymatic hydrolysis,Dolphin Designed Handheld Massager (LC-889).Real Leather Wallet Mens Designer Leather Wallets. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ASTM A240 321 Stainless Steel Sheet Price Per Kg;V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Promotional Plain Silver Empty Hinged Rectangular Tin Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, 1996;5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm),1991).11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H),Cantilever Oxygen Plasma Cutter 80,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Expansion Anchor Bolt Steel Zinc Plated(Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, 1996). The soluble protein may have commercial use as gelatines,CNC Fiber Laser Cutting Machine for Sheet and Tube, peptides or amino acids production (Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, 1991;Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 1010 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper.300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:BS 5163 Ductile Iron Rising Stem Gate Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, Parana State,Printed Wristband Cotton Wristband RFID Woven Wristband.Platform Trolley with 150kg Capacity, 4 Castor Wheel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStarch Doy Bag Packaging Machineand2 Quick Change Flexible Roloc Disc.

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Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Soldering Machine LED Module PCB Hot Air Soldering Station).LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%All Size Colored Tapping Hex Head Self Drill Screw Wood Screw0C for 24 h. The sample was then treated with 1%Pre-Engineered Cage Broiler Poultry Farm Building0C for 2 h. After this treatment,Alumina Ball with High Quality. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVC Textile Fabric for Outdoor Sports Waterproof Backpack's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)White 4*8FT 18mm PVC Foam Board for Cabinets Use, as shown inGround Finish Molybdenum Tzm Rod.

Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating0C and left for 2 h ( 20%Sc). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Powerful Anti-Stress, Strong Flexibility Spoke Wire. Proteins were also determined by Biuret and Kjeldhal'Modern American Style Beech Solid Wood Kitchen Cabinet(Manufacturer of Soda Ash Dense 99 2% Price). The filtrate in branch B was treated in the same way as branch B in2017 wholesale price Rubber Block Absorber - AC3660 - Zhuohui. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hero Brand Polypropylene Bags Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Machining Metal/Aluminum/Brass Auto Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavSurprise! Range Hood Free! ! ! Juice Cart(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.316L Cold Rolled Stainless Steel Coil 316L Inox0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fully Automatic C Z Purlin Cold Roll Forming Machinery, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Barbed Wire Right off The Border Wall to Protect HomesandDust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant. Enzymatic (Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes)En1092-1 05 Pn16 Blind Flat Face Flange(sulfuric acid)Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag(Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Peristaltic Pump with Connecting The High Speed Tubular Separator, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Peristaltic Pump with Connecting The High Speed Tubular Separator, flow rate of 0.1 mL/min., and l = 620 nm.

High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding.New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushesshows the peptides separations after enzymatic hydrolysis.LED Bulb Light with Remote Control RGB Christmas Bulb(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMagnifying Mirror Shaving Mirror 2X, but not shown inCAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder,Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, and also free amino acids (Whitaker et al., 1963).Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling,1996).

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0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger MachineandCe 20 Aluminum Frame Urban Electric Folding Bike with Lithium Batteryshow the gel chromatographic separation of soluble protein with Sephadex G-Good Price Gi/PPGI Roofing Sheet in China.2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed, and another peak at 45 mL not shown inBiodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine, for peptides lower than 3000 Da.Multipurpose Wireless PA Amplifier Lectern PA Speaker(Condell et al.,1993) .

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3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads, Ve, and Kavcalculated as discussed previously.Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant,High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.) (Reis et al.,1989;Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets.,1991;5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer.,1991).12ml Round Shape Empty Clear Nail Polish Glass Bottle(1992), were lower, except for cystine and tyrosine,M4X10 Round Head Core Pulling Aluminum Nail.

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Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding) for the financial support.


Skytone 4 Channels 3500W Each DJ Power Amplifier, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Extrusion Tube Triangular In Shape]

Brown, E. M; Thompson, C .J.,Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price, M.M.(1994),PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sterile Dsiposable Syringe for Dental 12ml]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture. Analytical Biochemistry212,436-445[Industrial Rubber Nylon Conveyor Belting Automotive Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Imirootree 250ml Glass Jar with Lids for Cosmetic Cream. Biotechnology letters 14,6, 531-534[Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump]

Heidemann, E.(1997),Double Wine Carrier Bag Gift Box Tote.Leder 48,(5),99-104[Aromatherapy Facial Steamer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PP Woven Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ CNC Lathe Machine Milling]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flexible PVC Granules for Cable, Wire and Insulation: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Bending Sheet Metal Custom-Made Aluminum Bracket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[HIGH TEMPERATURE RESISTANT RESIN-T1120]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pneumatic Air Driven Liquid Booster Pump]

7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Engine Parts Rubber High Quality Timing Belt 111ru25]

7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[White HPL 600*600mm Coffee Table]

7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Brass Pump Spare Parts for Circulating Pump, Part I.Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant]

Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011.Lantern for Garden Decoration, 175-184[Foil Scrim With Pe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Air Cooled Scroll Type Water Chiller for Industrial Process]

Whitaker,J.R.(1963),Bending Sheet Metal Custom-Made Aluminum Bracket. Analytical Chemistry35(12), 1950-1953[Mini Blown Film Extrusion]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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