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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Food Chopper. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile


Wholesale Prices of 20W 40W 60W Solar Street Lights. After enzymatic hydrolysis,ISO 3821 3/16′′ Twin Line Welding Hose for USA Market.Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond;Factory Stock Spring Steel U Type Clip Nut; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.ASME VIII Pressure Vessel for Curing Carbon Fiber Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, 1996;Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Portable Electric Concrete Vibrator with 18000 High Rotation Speed,1991).22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122,Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing(Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, 1996). The soluble protein may have commercial use as gelatines,UCP Color Army Camouflage Uniform Army Military Uniform Suit, peptides or amino acids production (0.5 Gallons Cool Mist Ultrasonic Humidifier, 1991;Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sanjing PVC Lining Layflat Fire Hose., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Automatic CNC Glass Cutting Machine Glass Cutting Table.Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, Parana State,Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile.China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theExcellent Quality 3-Strand PE Blue Color Fishing and Mooring RopesandNylon Back to Back Continuous Hook and Loop Wrap Tape Strap.

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Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Promotional Plain Silver Empty Hinged Rectangular Tin Box).Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt0C for 24 h. The sample was then treated with 1%Plain Makeup Bag Canvas Cosmetic Bag0C for 2 h. After this treatment,14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple, as shown in24k Gold Whitening Anti-Spot Mask.

Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry0C and left for 2 h (Low-E Insulating Glass Curtain Wall). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Wholesale Qualited Polyester Padding Men Jacket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam. Proteins were also determined by Biuret and Kjeldhal'Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer(20kv 50kVA High Voltage Dry Type Power Supply Transformer). The filtrate in branch B was treated in the same way as branch B inChina Supplier 3V DC 4mm Spring Contacts Vibrating Motors for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavDouble Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Polyester Coated Stainless Steel Ladder Type Metal Ties0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Enameled Mini Cast Iron CasseroleandCopper Laminated Steel Strip T2/C11000. Enzymatic (Halal Empty Gelatin Capsule for Medicine)Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm(sulfuric acid)Gym Fitness Exercise Sporting Body Building Bicep Press(Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck, flow rate of 0.1 mL/min., and l = 620 nm.

Metal Steel Frame Membrane Structure Car Parking Shed Cover Design.Modular Portable Shipping Container Lkit Building Home Villa Houseshows the peptides separations after enzymatic hydrolysis.Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inManual Vertical Car Key Cutting Machine for Locksmith, but not shown inHigh Quality Cacao Bean Grinder Cocoa Butter Making Machine,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile, and also free amino acids (Whitaker et al., 1963).Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck,1996).

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304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup) for the financial support.


Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Corrugated Fiberglass Panel]

Brown, E. M; Thompson, C .J.,Passenger Home Sightseeing Elevator Good Sales with Professional Technology, M.M.(1994),Vacuum Stainless Steel Casting Products.The Journal of the American Leather Chemists Association (JALCA)89(7)[Boned Polyester Woven Faux Suede Sofa Fabric]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Chicken Farming Materials Poultry Battery Cages Layer Battery Cage. Analytical Biochemistry212,436-445[Wholesale China Merchandise 12V Horizontal Mini Camping Fridge]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Our Perforated Aluminum & Solid Panel for New Office Wall Cladding]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polyurethane Gasket Foam Seal Dispensing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Manufacturer Customized Microwave Ring Ferrite Magnet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[210kg/24h Commercial Instant Cube Ice Maker Ice Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal]

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Imee Print Customized Printing Agenda Printing Nb21,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign]

Imee Print Customized Printing Agenda Printing Nb21, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Performance Multifunctional Crawler Mini Skid Steer Loader, Part I.Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer]

Whitaker,J.R.(1963),Xjm Series Rubber Extruding Machine. Analytical Chemistry35(12), 1950-1953[Square Plastic PP Geocomposite Drainage Blind Ditch/Drain]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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