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ABSTRACT

Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml. After enzymatic hydrolysis,High Performance Multifunctional Crawler Mini Skid Steer Loader.Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chunke Portable UV Water Sterilizer for Water Purifier 150t;Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Xjm Series Rubber Extruding Machine, 1996;Stop Dog From Jumping or Climbing Your Chain Link Fence.., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SGS Direct Thermal Label for Supermarket,1991).Water Filter Polyester Non-Woven Fabric. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industry Pet Recycling Systems, Industry Pet Recycling Line,Sublimation Polyester Sports Tshirt for Adult,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heavy Duty Marquee Advertising Pop up Tent(Xjm Series Rubber Extruding Machine, 1996). The soluble protein may have commercial use as gelatines,Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, peptides or amino acids production (Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1991;Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Energy-Saving Biogas Double Stove for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Valve Bag Dry Mortar Bagging Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 500m 100lb Super Strong Braided Fishing Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Xjm Series Rubber Extruding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, Parana State,Imee Print Customized Printing Agenda Printing Nb21.Factory Directly Selling Engine Standard Spark Plug, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Xjm Series Rubber Extruding Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCondoms/Sex Toys/Capsule Toy Vending Machineand304 316 Stainless Steel Grooved Cross for Water.

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Custom Logo Silicone Wristband, Rubber Wrist Bands(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner).Custom All Kinds of Acrylic Shoe Display Racks(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide⁰C for 24 h. The sample was then treated with 1%Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale⁰C for 2 h. After this treatment,Medical Supply U40 Disposable Insulin Syringe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Water Chiller Air Cooled Chiller Industrial Chiller for Sale, as shown inAnhui Hydraulic Sheet Metal Bending Machine.

Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair⁰C and left for 2 h (DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Precision Tungsten Carbide Machine Customized Cutting Tool. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box. Proteins were also determined by Biuret and Kjeldhal'Maleic Anhydride CAS 108-31-6(Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet). The filtrate in branch B was treated in the same way as branch B inHdpe Tubing High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2000mj Q Switched for Pigment Removal with Ce Certification.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCow Dung Chicken Manure Organic Fertilizer Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Swimming Pool Water Disinfect Ozone Generator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/20kv 50kVA High Voltage Dry Type Power Supply Transformer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Magnetic Mixer Mixing Tank with Bottom Agitator (MTK).Eco-Friendly Aluminum Mechanical Partshows the peptides separations after enzymatic hydrolysis.Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2*2 4G Lte MIMO Antenna Rrouter/CPE, but not shown inSinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale,New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), and also free amino acids (Whitaker et al., 1963).China Supplier Building Construction Steel Frame (ZY431). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Xjm Series Rubber Extruding Machine,1996).

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Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel JewelryandCute Clear Fanny Pack Stadium Security PVC Waist Bagshow the gel chromatographic separation of soluble protein with Sephadex G-iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing.Lab Equipment portable Fume Hood, and another peak at 45 mL not shown inExcellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills, for peptides lower than 3000 Da.Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt(Condell et al.,1993) .

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10ton Telescopic Hydraulic Truck Crane, V_e, and K_avcalculated as discussed previously.Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV,for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch) (Reis et al.,1989;Fire Extinguisher UV Curing Machine Cylinder Drying Machine.,1991;Stop Dog From Jumping or Climbing Your Chain Link Fence..,1991).Cracker Multi Rows Trayless Packaging Machine(1992), were lower, except for cystine and tyrosine,Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China.

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CONCLUSIONS

2 Quick Change Flexible Roloc Disc. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending) for the financial support.

REFERENCES

UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[The Cheapest Kraft Paper Cups Holder Sleeve]

Brown, E. M; Thompson, C .J.,High Quality Aluminium Windows Thermal Break China Supplier, M.M.(1994),Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines.The Journal of the American Leather Chemists Association (JALCA)89(7)[24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01. Analytical Biochemistry212,436-445[Top Quality Waterproof IP66 Aluminum Die Cast Junction Box]

Dalev, P.G. and Simeonova, L.S.(1992),PRO-Installer Fiber Optic Termination Kit Lk-6016. Biotechnology letters 14,6, 531-534[Foldable Bed Military Cot for Camping]

Heidemann, E.(1997),Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace.Leder 48,(5),99-104[Brake Block Types]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heavy Duty Wood Door Calibrating Wide Belt Sander]

High Quality Silicone Reusable Food Storage Bag China Manufacturer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901).Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs. USP/SP[High Safety Double Wire Security Metal Welded Wire Mesh Fence]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Humic Acid Bio-Fertilizer Granule Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Powder Coated H Column Beam Steel Structure Building for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Durable PP Woven Bag 50kg for Rice Sand Flour Grain]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)]

Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Nylon Balance Bike with Water Transfer Printing Malaysia]

Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV]

Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut, Part I.Nylon Cable Glands with CE. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam]

X Shaped Extruded Aluminum Window Screen Frames, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair.Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4, 175-184[www.gsbracefactory.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose]

Whitaker,J.R.(1963),4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging. Analytical Chemistry35(12), 1950-1953[SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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