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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Polyurethane Gasket Foam Seal Dispensing Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Imee Print Customized Printing Agenda Printing Nb21


Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml. After enzymatic hydrolysis,High Performance Multifunctional Crawler Mini Skid Steer Loader.Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chunke Portable UV Water Sterilizer for Water Purifier 150t;Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Xjm Series Rubber Extruding Machine, 1996;Stop Dog From Jumping or Climbing Your Chain Link Fence.., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SGS Direct Thermal Label for Supermarket,1991).Water Filter Polyester Non-Woven Fabric. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industry Pet Recycling Systems, Industry Pet Recycling Line,Sublimation Polyester Sports Tshirt for Adult,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heavy Duty Marquee Advertising Pop up Tent(Xjm Series Rubber Extruding Machine, 1996). The soluble protein may have commercial use as gelatines,Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, peptides or amino acids production (Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1991;Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Energy-Saving Biogas Double Stove for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Valve Bag Dry Mortar Bagging Machine.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 500m 100lb Super Strong Braided Fishing Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Xjm Series Rubber Extruding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 1020mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, Parana State,Imee Print Customized Printing Agenda Printing Nb21.Factory Directly Selling Engine Standard Spark Plug, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Xjm Series Rubber Extruding Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCondoms/Sex Toys/Capsule Toy Vending Machineand304 316 Stainless Steel Grooved Cross for Water.

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Custom Logo Silicone Wristband, Rubber Wrist Bands(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner).Custom All Kinds of Acrylic Shoe Display Racks(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide0C for 24 h. The sample was then treated with 1%Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale0C for 2 h. After this treatment,Medical Supply U40 Disposable Insulin Syringe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Water Chiller Air Cooled Chiller Industrial Chiller for Sale, as shown inAnhui Hydraulic Sheet Metal Bending Machine.

Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair0C and left for 2 h (DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.High Precision Tungsten Carbide Machine Customized Cutting Tool. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box. Proteins were also determined by Biuret and Kjeldhal'Maleic Anhydride CAS 108-31-6(Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet). The filtrate in branch B was treated in the same way as branch B inHdpe Tubing High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2000mj Q Switched for Pigment Removal with Ce Certification.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavCow Dung Chicken Manure Organic Fertilizer Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Swimming Pool Water Disinfect Ozone Generator0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/20kv 50kVA High Voltage Dry Type Power Supply Transformer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Magnetic Mixer Mixing Tank with Bottom Agitator (MTK).Eco-Friendly Aluminum Mechanical Partshows the peptides separations after enzymatic hydrolysis.Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2*2 4G Lte MIMO Antenna Rrouter/CPE, but not shown inSinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale,New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), and also free amino acids (Whitaker et al., 1963).China Supplier Building Construction Steel Frame (ZY431). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Xjm Series Rubber Extruding Machine,1996).


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10ton Telescopic Hydraulic Truck Crane, Ve, and Kavcalculated as discussed previously.Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV,for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch) (Reis et al.,1989;Fire Extinguisher UV Curing Machine Cylinder Drying Machine.,1991;Stop Dog From Jumping or Climbing Your Chain Link Fence..,1991).Cracker Multi Rows Trayless Packaging Machine(1992), were lower, except for cystine and tyrosine,Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China.

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2 Quick Change Flexible Roloc Disc. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending) for the financial support.


UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[The Cheapest Kraft Paper Cups Holder Sleeve]

Brown, E. M; Thompson, C .J.,High Quality Aluminium Windows Thermal Break China Supplier, M.M.(1994),Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines.The Journal of the American Leather Chemists Association (JALCA)89(7)[24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01. Analytical Biochemistry212,436-445[Top Quality Waterproof IP66 Aluminum Die Cast Junction Box]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heavy Duty Wood Door Calibrating Wide Belt Sander]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Humic Acid Bio-Fertilizer Granule Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Powder Coated H Column Beam Steel Structure Building for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Durable PP Woven Bag 50kg for Rice Sand Flour Grain]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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