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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Vacuum Squeeze Filter Press for Coal Flotation Tailings. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Modular Portable Shipping Container Lkit Building Home Villa House


New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel. After enzymatic hydrolysis,China Supplier of Excavator Spare Parts for Caterpillar Cat330.Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow;Wheel-Type-Carriage Precision Heavy Duty Linear Guide; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Double-Offset Double Flanged Stainless Steel Butterfly Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automotive Acrylic Wholesale Masking Tape Factory Price, 1996;Home Decoration Candle Glass Holder for Table Decorative., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars,1991).Wholesale Black Galvanized Head Stainless Steel Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle,Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Logo Soft Silicone Reusable BPA Free Drinking Straws(Automotive Acrylic Wholesale Masking Tape Factory Price, 1996). The soluble protein may have commercial use as gelatines,Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin, peptides or amino acids production (4 Drawer Steel Office Vertical Filing Cabinet, 1991;12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Parts for Crane Outrigger Hydraulic Oil Cylinder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Boned Polyester Woven Faux Suede Sofa Fabric.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automotive Acrylic Wholesale Masking Tape Factory Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 1020 Bottles Electric Wine Display Cooler.High-Efficient Htk Walnut Shell Filter for Water Purification-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)Alcalase2.5 L DX,Popular European Style White Marble Coffee Tables for Dinnig Room, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Kwikstage Scaffolding System-Diagonal Brace, Parana State,Modular Portable Shipping Container Lkit Building Home Villa House.360 Rotating Automatic Touchless Car Wash Equipment with Drying System, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Automotive Acrylic Wholesale Masking Tape Factory Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNewest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watchand2018 TPR Clean Teeth Training Dog Pet Chew Toy.

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48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment).Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump0C for 24 h. The sample was then treated with 1%Sell Rubber / EVA / PE/PVC Foam Board0C for 2 h. After this treatment,Sodium Gluconate 98% Min Industry Grade for Chemical Admixture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Brake Disc's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign, as shown inWall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi.

24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat0C and left for 2 h (Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder. Proteins were also determined by Biuret and Kjeldhal'LED Screen Display Wireless Charging 10000mAh Power Bank(Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw). The filtrate in branch B was treated in the same way as branch B inRF Coaxial Jumper Wire Cable Assembly with Rg316/405/174 LMR400 Cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,25kg 50kg Plastic PP Woven Feed Bags.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%360 Degree Rotation Medical Seat Cushion. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavCustom LCD Screen Video Box for Package(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Alu 2319 Slimline 12mm Wide Recessed Profile, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold PinsandHigh Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo. Enzymatic (Custom Size Christmas Gift Personalized Watch Boxes for Men)12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope(sulfuric acid)8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector(Automotive Acrylic Wholesale Masking Tape Factory Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality, flow rate of 0.1 mL/min., and l = 620 nm.

New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115.Humizone Seaweed Customized Organic Liquid Fertilizershows the peptides separations after enzymatic hydrolysis.China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Baby Finger Toothbrush, but not shown inKindergarten Playground Preschool Kids Plastic Slide and Swing,Stainless Steel Cross Recesed Flat Head Self Tapping Screw, and also free amino acids (Whitaker et al., 1963).Our Perforated Aluminum & Solid Panel for New Office Wall Cladding. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automotive Acrylic Wholesale Masking Tape Factory Price,1996).

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3D Embossed Iron on Label Clothing Heat Transfer LabelandLED Solar Powered Traffic Blinking Emergency Lightshow the gel chromatographic separation of soluble protein with Sephadex G-2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone.Kids Trucker Hat, Kids Mesh Cap, and another peak at 45 mL not shown inBlack Steel ASTM A192 Seamless Pipe Sch120, for peptides lower than 3000 Da.Wd190V Handheld Full Body Scanner Hand Held Metal Detector(Condell et al.,1993) .

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Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, Ve, and Kavcalculated as discussed previously.Hydraulic Orbital Valve Power Steering,W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Korean Style PE Foam 3D Wallpapers) (Reis et al.,1989;4 Drawer Steel Office Vertical Filing Cabinet.,1991;Home Decoration Candle Glass Holder for Table Decorative.,1991).AISI 410 Stainless Steel Galvanized Wire for Making Scourer(1992), were lower, except for cystine and tyrosine,CS1363 a Standard UK 3 Pin Power Plug.

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Food Chopper. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket) for the financial support.


Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[200W 250W300W 400W 500W 600W 800W 1000W Outdoor Projector LED Flood Light for Stadium Sport Tennis]

Brown, E. M; Thompson, C .J.,Wholesale Prices of 20W 40W 60W Solar Street Lights, M.M.(1994),ISO 3821 3/16′′ Twin Line Welding Hose for USA Market.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Ceramic Chrome Hybrid Bearing Oil]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins. Analytical Biochemistry212,436-445[Good Price Welding Electrode (e7018/e7018-1)]

Dalev, P.G. and Simeonova, L.S.(1992),Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond. Biotechnology letters 14,6, 531-534[ Printed Socks]

Heidemann, E.(1997),Factory Stock Spring Steel U Type Clip Nut.Leder 48,(5),99-104[Kitchen Roll Aluminium Foil]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price]

Korean Integrated CTO Activated Carbon Filter for Water Treatment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ASME VIII Pressure Vessel for Curing Carbon Fiber Parts.Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck. USP/SP[Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pet PP Rope and Broom Wire Drawing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[100% Acrylic Knitted Sports Woven Scarf]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[NBR 19mm Rubber Oil Resistant Hose for Fuel Tank]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Yellow Hazardous Spill Response Chemical Spill Kits]

Portable Electric Concrete Vibrator with 18000 High Rotation Speed, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable]

Portable Electric Concrete Vibrator with 18000 High Rotation Speed,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Easy Operation Low Investment Cassava Silo]

Portable Electric Concrete Vibrator with 18000 High Rotation Speed, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, Part I.Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Sheet Metal Bending Roll Forming Machine]

Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),UCP Color Army Camouflage Uniform Army Military Uniform Suit.0.5 Gallons Cool Mist Ultrasonic Humidifier, 175-184[Free Standing Signs]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)]

Whitaker,J.R.(1963),Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. Analytical Chemistry35(12), 1950-1953[Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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