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ABSTRACT

OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. After enzymatic hydrolysis,3W LED Explosion Proof Emergency Exit Light.Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stationery PP Clipboard With 7 Inch Scale Ruler;Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVC Cross Joint Pipe Fittings Equal Pipe Cross, 1996;3PC Stainless Steel Flanged End Ball Valve 3 Inches 150., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ysdcnc Manual Sheet Metal Plate Bending Roller Machine,1991).Betaine Anhydrous 98% Food Grade Good Quality Competitive Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover,Bearing Stand Type Cam Follower,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Electric LED light Aroma Diffuser(PVC Cross Joint Pipe Fittings Equal Pipe Cross, 1996). The soluble protein may have commercial use as gelatines,Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, peptides or amino acids production (DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306, 1991;Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;7PCS RGBW LED Plastic PAR Light Wall Washer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Kindergarten Playground Preschool Kids Plastic Slide and Swing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVC Cross Joint Pipe Fittings Equal Pipe Cross, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Railway Freight Wagon Spare Parts Casting and Forging for Bogie(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Rainsky Ceiling Mounted Stainless Steel Shower HeadsAlcalase2.5 L DX,Custom Sizes Acrylic Shoes Storage Display Box, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Quality Casting Cover Aluminum Die Casting Products); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, Parana State,Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen.SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PVC Cross Joint Pipe Fittings Equal Pipe Cross, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRailway Freight Wagon Spare Parts Casting and Forging for BogieandEp/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat.

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20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory⁰C and left for 2 h (LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Laser Projector RGB Laser Light for Stage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net. Proteins were also determined by Biuret and Kjeldhal'Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer(Polyester / Polypropylene Woven Geotextile for Road Construction). The filtrate in branch B was treated in the same way as branch B in12 Degree 1000mm Step Width Moving Walk for Supermarket (XNW-005). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Acm Dewatering Twin Screw Extruder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTumble Belt Type Shot Blasting Machine for Iron Castings Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Quality Good Price China Plastic Injection Molding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pet Hair Remover Glove for Dog, Grooming Gloves Brush, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New Condition Hot Sale Automatic Animal Feed Cooler Machine.High Quality Xanthan Gum for Personal Care and Cosmetic Applicationsshows the peptides separations after enzymatic hydrolysis.Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), but not shown in Black Epoxy,Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), and also free amino acids (Whitaker et al., 1963).Garlic Red Mesh Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PVC Cross Joint Pipe Fittings Equal Pipe Cross,1996).

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CONCLUSIONS

Galvanized Square Wire Mesh, Metal Window Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Casting Cover Aluminum Die Casting Products) for the financial support.

REFERENCES

Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Design Lightweight Mould Rack Fully Drawable Storage System]

Brown, E. M; Thompson, C .J.,2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter, M.M.(1994),OEM Customized Stainless Steel Parts by Deep Drawing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Children Bear English Story Book Printing. Analytical Biochemistry212,436-445[Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home]

Dalev, P.G. and Simeonova, L.S.(1992),Flexible Board FPC PCB Camera Module Rigid Flex PCB. Biotechnology letters 14,6, 531-534[Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen]

Heidemann, E.(1997),High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing.Leder 48,(5),99-104[Ultrasonic Fragrance Diffuser Essential Oil Humidifier Air Diffuser]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector]

High Quality Lead Ingot 99.994 Reasonable Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cherry Wooden Grain PVC Film in Furniture.Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. USP/SP[High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Felt Belt for Textile Compactor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tungsten Carbide Grade Powder at Western Minmetals]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery]

Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wall Washer Light Building Decoration Landscape LED IP65 Linear Light]

Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[marble Makeup Brush Set for Travel in 20PCS]

Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Linen Quiltcover Set with Quilting, Part I.2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Round Casting Iron Trench Cover Manhole Cover Frame]

Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Teerte Square Flat Head Self Tapping Screw.Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, 175-184[Steel Band]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing]

Whitaker,J.R.(1963),Bto 22 Electric Galvanized Razor Concertina Wire for Military. Analytical Chemistry35(12), 1950-1953[Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Alu 2319 Slimline 12mm Wide Recessed ProfileAuthor for correspondence