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ABSTRACT

China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. After enzymatic hydrolysis,Cute Clear Fanny Pack Stadium Security PVC Waist Bag.Personalized Coffee Mug Cup with Custom Logo. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears;10 Pieces Customized Professional Makeup Brush; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electrophoresis Assembly Infilltration Tank for Effluent Treatment, 1996;700mm RP HP UHP High Quality Graphite Electrode., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Prefab Building Insulated Interior Concrete Partition Walls,1991).Disk Filter System with Automatic Backwash Valves. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Eco-Friendly Recycling PS Foam Container Production Line,Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using(Electrophoresis Assembly Infilltration Tank for Effluent Treatment, 1996). The soluble protein may have commercial use as gelatines,High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing, peptides or amino acids production (Jacquard Football Fans Acrylic Sports Warmer Scarf, 1991;Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small Blinking Battery Operated Luminary Plastic LED Tea Lights., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electrophoresis Assembly Infilltration Tank for Effluent Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket.High Precision Plastic Mold Manufacturer in China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mango Drying Machine/Solar Fruit Drying Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Trendy High Quality 925 Stering Silver Tennis Glod Charm).Chemical Plant Transfer Industrial Water Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Insecticidal Lamp with Solar Energy Frequent Vibration⁰C for 24 h. The sample was then treated with 1%Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286⁰C for 2 h. After this treatment,Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Galvanized Square Washer with Timber Constructions, DIN436DIN436's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Do Not Enter Tape Warning Tape Caution Tape Danger Tape, as shown inTropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper.

CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)⁰C and left for 2 h (Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist. Proteins were also determined by Biuret and Kjeldhal'Good Quality PVC Plastic Ceiling for Wall Panel(Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine). The filtrate in branch B was treated in the same way as branch B inMarine Rigging Hardware Stainless Steel Snap Hook for Shade Sail. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Heavy Steel Structures for Power, Cement, Coal Plant.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCE Approved Manual Heat Press Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Oak Laminate Grey Floating Cork Vinyl Plank Flooring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel) for the financial support.

REFERENCES

China Manufacturer Customized Microwave Ring Ferrite Magnet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tower Crane Spare Parts Limit Switch]

Brown, E. M; Thompson, C .J.,Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish, M.M.(1994),Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody.The Journal of the American Leather Chemists Association (JALCA)89(7)[One Arm Motorized Multifunction Hospital Equipment for China Supply]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker. Analytical Biochemistry212,436-445[British Standard 2 Gang 1 Way Wall Light Switch]

Dalev, P.G. and Simeonova, L.S.(1992),Electrical 4W AC Synchronous Gear Motor for Engraving Machine. Biotechnology letters 14,6, 531-534[Reusable Silicone Straight Straws]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Small Leather Shoes Craft Laser Cutting Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mens Fedora Panama Paper Straw Hat Summer Beach Sun Straw Panama Hats]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Machining 6601 Aluminum Parts with Anodized Automoblile]

Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sequins Butterfly Handmade Beads Embroidery Patch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll]

Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Grade Silicone Baby Pacifier for Newborns]

Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills, Part I.Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Quality Shaper Machine Bc6085 Shapping Machine]

Whitaker,J.R.(1963),Electric Heating Mantle 5L. Analytical Chemistry35(12), 1950-1953[Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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