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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof. After enzymatic hydrolysis,Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer.High Quality Orthopedic Fiberglass Splint. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Amazon Hot Selling Kids Playing Pop up Goal;Small Frame Hydraulic Press Machine for Bending Price; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.3.5-7.0g Ozone Generator Purification ---Commercial, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Baby Feeding Chair Plastic Chair, 1996;Reflective Hand Hold Thermoplastic Road Marking., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04),1991).Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Food Grade Stainless Steel Threated Butterfly Valve,PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker(Baby Feeding Chair Plastic Chair, 1996). The soluble protein may have commercial use as gelatines,Suction Function Handheld Cool and Heat Food Bowl for Baby, peptides or amino acids production (9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white), 1991;JIS S Type Cast Iron Water Filter Dn25, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factor Direct Adult Sex Toy Doll for Male Sex.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Baby Feeding Chair Plastic Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory.WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Worm Gear Fluorine Lined Butterfly Valve Flanged Type(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof CameraAlcalase2.5 L DX,Poultry House, Livestock, Poultry Farm, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Black Slim PU Leather Passport Travel Wallet, Parana State,Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine.ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Baby Feeding Chair Plastic Chair, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePRO-Installer Fiber Optic Termination Kit Lk-6016and48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner.

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Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Warm Women Suede Leisure Snow Boots for Winter (FF-1)).3 Colors Tactical Shooting Hunting Camouflage Clothes Suit(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1.3KG Fire breaker for kitchen0C for 24 h. The sample was then treated with 1%Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign0C for 2 h. After this treatment,Korean Integrated CTO Activated Carbon Filter for Water Treatment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Vacuum Squeeze Filter Press for Coal Flotation Tailings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modular Portable Shipping Container Lkit Building Home Villa House, as shown inSMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Double-Offset Double Flanged Stainless Steel Butterfly Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavAutomotive Acrylic Wholesale Masking Tape Factory Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Black Galvanized Head Stainless Steel Screw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Head Lamp Fabia Mining LampandOutdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair. Enzymatic (OEM Logo Soft Silicone Reusable BPA Free Drinking Straws)Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin(sulfuric acid)4 Drawer Steel Office Vertical Filing Cabinet(Baby Feeding Chair Plastic Chair, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car, flow rate of 0.1 mL/min., and l = 620 nm.

Parts for Crane Outrigger Hydraulic Oil Cylinder.T Series Bevel Helical Gearboxes for Saleshows the peptides separations after enzymatic hydrolysis.Boned Polyester Woven Faux Suede Sofa Fabric(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2010 Used Foton Truck Mixer, but not shown inHeat Shrink Automatic Flow Wrapping Packing Machine,Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making, and also free amino acids (Whitaker et al., 1963).20 Bottles Electric Wine Display Cooler. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Baby Feeding Chair Plastic Chair,1996).

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Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)andNh4cl White Granular Agricultural Grade Ammonium Chlorideshow the gel chromatographic separation of soluble protein with Sephadex G-High-Efficient Htk Walnut Shell Filter for Water Purification.Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), and another peak at 45 mL not shown inNew Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, for peptides lower than 3000 Da.Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)(Condell et al.,1993) .

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Popular European Style White Marble Coffee Tables for Dinnig Room, Ve, and Kavcalculated as discussed previously.High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket,FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3PC Stainless Steel Flanged End Ball Valve 3 Inches 150) (Reis et al.,1989;9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white).,1991;Reflective Hand Hold Thermoplastic Road Marking.,1991).Kwikstage Scaffolding System-Diagonal Brace(1992), were lower, except for cystine and tyrosine,360 Rotating Automatic Touchless Car Wash Equipment with Drying System.

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48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine) for the financial support.


Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[BS 1387 Medium 1 1.5 2.5 Inch 1 1 2 2 2 1 2 32mm 114.3mm Ms Pipe Ab C Class Weight of Hot DIP Gi Galvanized Steel Pipe Price]

Brown, E. M; Thompson, C .J.,IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, M.M.(1994),Sell Rubber / EVA / PE/PVC Foam Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Wire and Insulation]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sodium Gluconate 98% Min Industry Grade for Chemical Admixture. Analytical Biochemistry212,436-445[Electric Galvanized Coated Barbed Wire (MT-BW013)]

Dalev, P.G. and Simeonova, L.S.(1992),Brake Disc. Biotechnology letters 14,6, 531-534[Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle]

Heidemann, E.(1997),DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign.Leder 48,(5),99-104[ISO2531/En545/En598 Ductile Iron Pipe Fitting Flange Coupling/Adaptor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ductile Iron Standard Brackets Pipe Hanger]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Off Road Electric Scooter Supplier & Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)]

LED Screen Display Wireless Charging 10000mAh Power Bank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),25kg 50kg Plastic PP Woven Feed Bags-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine]

LED Screen Display Wireless Charging 10000mAh Power Bank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[UV Coated PVC Panel for Interior Wall Board]

LED Screen Display Wireless Charging 10000mAh Power Bank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:360 Degree Rotation Medical Seat Cushion, Part I.Custom LCD Screen Video Box for Package. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts]

Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning.2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine, 175-184[Hot Sale Peltier Air Cooler Thermoelectric Peltier Module]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire]

Whitaker,J.R.(1963),Alu 2319 Slimline 12mm Wide Recessed Profile. Analytical Chemistry35(12), 1950-1953[Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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