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ABSTRACT

Wvrb Series Planetary Gearbox Used for Robot. After enzymatic hydrolysis,NPK 15 15 15 Compound Fertilizer at a Hot Selling.Mini Blown Film Extrusion. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality PVC Resin with Low Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Backhoe Loader with Air Conditioning, 1996;Line Scan Uvss System Portable Under Vehicle Surveillance., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light,1991).FSTN Type Monochrome Graphic Display Cog LCD with Backlight. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3D Embossed Iron on Label Clothing Heat Transfer Label,Glass Food Jar & Plastic Lid,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer(Small Backhoe Loader with Air Conditioning, 1996). The soluble protein may have commercial use as gelatines,Easy Operation Low Investment Cassava Silo, peptides or amino acids production (CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, 1991;100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Building Material High Quality 316 Stainless Steel Grating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sc-2400 Automatic Multi Function Sosn Side Drilling Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Backhoe Loader with Air Conditioning, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair.Quality Shaper Machine Bc6085 Shapping Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Thermoplastic Road Marking Paint Glass Beads(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Free Sample! AC Power Contactor for Sale, Parana State,Round Shape All Color Pets Feeding Dog Bowl of China Supplies.PA System Loudspeaker 100W Outdoor Column Speaker , exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Small Backhoe Loader with Air Conditioning, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChicken Farming Materials Poultry Battery Cages Layer Battery CageandOPP Printed Block Bottom Bags.

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Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump).Breathable Surgical Disposable 3 Ply Earloop Face Mask(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9⁰C for 24 h. The sample was then treated with 1%Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw⁰C for 2 h. After this treatment,Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flower Design Custom Made Silk Scarf's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Design Indoor Artificial Mini Rockery Fountains, as shown inHot Sale Electric LED light Aroma Diffuser.

7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane⁰C and left for 2 h (Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Medical Endoscope Disposable Bite Block with Bandage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels. Proteins were also determined by Biuret and Kjeldhal'Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)(Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer). The filtrate in branch B was treated in the same way as branch B inShoe Lamination Machine EVA Lamination Machine for Export. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ladder Type Cable Tray with Ce/ GOST/ TUV/UL.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFine Bubble Diffuser for Air Purifier(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Cheap Price Custom Aluminum Logo Metal Label Plate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3120/17/38 Boltable Crane Rail Clips, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder MillandChina Supplier Rubber Injection Molding Machine with Ce. Enzymatic (Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China)Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery(sulfuric acid)High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free(Small Backhoe Loader with Air Conditioning, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, flow rate of 0.1 mL/min., and l = 620 nm.

Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment.Auto Spare Parts 12629037 Ignition Coil for Cadillacshows the peptides separations after enzymatic hydrolysis.High Quality & High Speed Straight Line Wire Drawing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, but not shown inSt1000 Fire Resistant Steel Cord Rubber Belt,High Speed 3000W Fiber Laser Cutter, and also free amino acids (Whitaker et al., 1963).Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Small Backhoe Loader with Air Conditioning,1996).

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UV Coated PVC Panel for Interior Wall Board, V_e, and K_avcalculated as discussed previously.Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.,Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts) (Reis et al.,1989;CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making.,1991;Line Scan Uvss System Portable Under Vehicle Surveillance.,1991).Wholesale 30W LED Down Lights with Budget Price(1992), were lower, except for cystine and tyrosine,Embossed Textured Wallpaper for Solvent Printing.

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CONCLUSIONS

Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case) for the financial support.

REFERENCES

4-14 Manual Staple Gun with Staples for Hand Tools, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10A MPPT Solar Controllers PV 60V for off-Grid]

Brown, E. M; Thompson, C .J.,500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3), M.M.(1994),Smallest Portable Waste Transformer Oil Purifier Machine Zy-6.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Laser Projector RGB Laser Light for Stage]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles. Analytical Biochemistry212,436-445[Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed Automatic Packing Machine (GK-650 BA). Biotechnology letters 14,6, 531-534[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

Heidemann, E.(1997),5000-5900MHz 4G Built-in Automotive Antenna.Leder 48,(5),99-104[U Arm]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging]

CNC Machining 6601 Aluminum Parts with Anodized Automoblile, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Glass Lined Reactor for Chemical Factory.OEM Metal Stamping Stainless Steel Parts with CNC Machining. USP/SP[ID25mm Od86mm Standard Through Hole Slip Ring]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Dual Probe Ph Meter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Comfortable Two Piece Women Fitness Clothing for Gym Wear]

Supermarket Plastic T-Wire Basket Swing up Price Label Holder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox]

Supermarket Plastic T-Wire Basket Swing up Price Label Holder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck]

Supermarket Plastic T-Wire Basket Swing up Price Label Holder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, Part I.Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)]

Degradable PLA Plastic Bottle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale China Merchandise 12V Horizontal Mini Camping Fridge.20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House, 175-184[High Quality Cable Extrusion Machine Mold]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe]

Whitaker,J.R.(1963),No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet. Analytical Chemistry35(12), 1950-1953[Stainless Steel Expansion Anchor Bolt Steel Zinc Plated]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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