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ABSTRACT

Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm). After enzymatic hydrolysis,11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale.Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cantilever Oxygen Plasma Cutter 80;Stainless Steel Expansion Anchor Bolt Steel Zinc Plated; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Fiber Laser Cutting Machine for Sheet and Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, 1996;Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole,1991).Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump,10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction(Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, 1996). The soluble protein may have commercial use as gelatines,BS 5163 Ductile Iron Rising Stem Gate Valve, peptides or amino acids production (CNC Aluminum Sheet Press Brake, 1991;ID25mm Od86mm Standard Through Hole Slip Ring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bio Media.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Platform Trolley with 150kg Capacity, 4 Castor Wheel.Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

White 4*8FT 18mm PVC Foam Board for Cabinets Use, Parana State,5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer.Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMagic Sequin Pouch Coin Pouch Kids Sequin Coin PurseandReflective Hand Hold Thermoplastic Road Marking.

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Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2000mj Q Switched for Pigment Removal with Ce Certification).Powerful Anti-Stress, Strong Flexibility Spoke Wire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Modern American Style Beech Solid Wood Kitchen Cabinet⁰C for 24 h. The sample was then treated with 1%Hero Brand Polypropylene Bags Machine⁰C for 2 h. After this treatment,CNC Machining Metal/Aluminum/Brass Auto Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Surprise! Range Hood Free! ! ! Juice Cart's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chicken Layer Cage Poultry Cage Chicken Cages, as shown inFull Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000).

316L Cold Rolled Stainless Steel Coil 316L Inox⁰C and left for 2 h (Industrial Activated Carbon Water Filter Industry Water Treatment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fully Automatic C Z Purlin Cold Roll Forming Machinery. Proteins were also determined by Biuret and Kjeldhal'Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant(Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen). The filtrate in branch B was treated in the same way as branch B inLuyao Electric Neck Support Massage Pillow Deep Kneading Back Cushion. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%En1092-1 05 Pn16 Blind Flat Face Flange. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED Bulb Light with Remote Control RGB Christmas Bulb, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant.Brass Pump Spare Parts for Circulating Pumpshows the peptides separations after enzymatic hydrolysis.High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inInterior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel, but not shown inCreative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir,12ml Round Shape Empty Clear Nail Polish Glass Bottle, and also free amino acids (Whitaker et al., 1963).M4X10 Round Head Core Pulling Aluminum Nail. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets,1996).

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PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels, V_e, and K_avcalculated as discussed previously.2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture,Imirootree 250ml Glass Jar with Lids for Cosmetic Cream. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115) (Reis et al.,1989;CNC Aluminum Sheet Press Brake.,1991;Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security.,1991).Double Wine Carrier Bag Gift Box Tote(1992), were lower, except for cystine and tyrosine,(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts.

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CONCLUSIONS

Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Alumina Ball with High Quality) for the financial support.

REFERENCES

Flexible PVC Granules for Cable, Wire and Insulation, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.mutrade.com]

Brown, E. M; Thompson, C .J.,7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security, M.M.(1994),Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Brass Pump Spare Parts for Circulating Pump. Analytical Biochemistry212,436-445[AVR Se350 Automatic Voltage Regulator for Marathon Alternator]

Dalev, P.G. and Simeonova, L.S.(1992),Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes. Biotechnology letters 14,6, 531-534[Eco-Friendly Aluminum Mechanical Part]

Heidemann, E.(1997),Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi.Leder 48,(5),99-104[Soft-Touch Matte Golden Plastic Jars/Bottles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2*2 4G Lte MIMO Antenna Rrouter/CPE]

Promotional Plain Silver Empty Hinged Rectangular Tin Box, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011.Lantern for Garden Decoration. USP/SP[Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Chunke Portable UV Water Sterilizer for Water Purifier 150t]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bending Sheet Metal Custom-Made Aluminum Bracket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Galvanized Coil Suppliers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cute Clear Fanny Pack Stadium Security PVC Waist Bag]

New Shacman F3000 6X4 Truck Trailer Head, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills]

New Shacman F3000 6X4 Truck Trailer Head,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Home Kitchen Water Purification Ozone Generator 1000mg]

New Shacman F3000 6X4 Truck Trailer Head, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention, Part I.Cylindrical Shape Beer Glass Bottle for Wine Storage. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Plastic Injection for OEM Product, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024.Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event, 175-184[Factory Supply 4ml 5ml 6ml 7ml 8ml 10ml 15ml 20ml 30ml 35ml Clear Amber Ampoule Injection Penicillin Glass Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag]

Whitaker,J.R.(1963),Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen. Analytical Chemistry35(12), 1950-1953[Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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