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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Acm Dewatering Twin Screw Extruder

ABSTRACT

Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). After enzymatic hydrolysis,Tumble Belt Type Shot Blasting Machine for Iron Castings Parts.Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Alumina Balls for Hydogen Peroxide, 1996;Anti-High Pressure Insulated Pliers with PVC Dipped Handles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Selling Electronical Blue Pet Plastic Release Film Price,1991).DIN127 Stainless Steel Spring Lock Washer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom,New Condition Hot Sale Automatic Animal Feed Cooler Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System(Alumina Balls for Hydogen Peroxide, 1996). The soluble protein may have commercial use as gelatines,Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144), peptides or amino acids production (Garlic Red Mesh Bag, 1991;Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ground Finish Molybdenum Tzm Rod., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Transparent/Clear BOPP Jumbo Roll.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Alumina Balls for Hydogen Peroxide, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chenille Microfiber Mop Head.Custom Waterproof Pizza Sign Large Advertising Signboard-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Shrink Sleeve Label for Drinking Water Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM Customized Stainless Steel Parts by Deep Drawing, Parana State,Acm Dewatering Twin Screw Extruder.Children Bear English Story Book Printing, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Alumina Balls for Hydogen Peroxide, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)andAtf-805 Auto Transmission System Flushing Machine with CE/SGS.

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Flexible Board FPC PCB Camera Module Rigid Flex PCB(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238).High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cherry Wooden Grain PVC Film in Furniture⁰C for 24 h. The sample was then treated with 1%Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine⁰C for 2 h. After this treatment,Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump, as shown inCuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair.

Linen Quiltcover Set with Quilting⁰C and left for 2 h (Aluminum Low Profile Adjustable Bracket). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace. Proteins were also determined by Biuret and Kjeldhal'Teerte Square Flat Head Self Tapping Screw(Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump). The filtrate in branch B was treated in the same way as branch B inWinter Plain Color Kids Shoes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bto 22 Electric Galvanized Razor Concertina Wire for Military. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Selling Military Large Event Marquee Tent Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High-Quality Pre-Seasoned Cast Iron Wok, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising PosterandRebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. Enzymatic (Marine High Visibility Solas Reflective Tape)24L Thermoelectric Cooler and Warmer Car Mini Car Fridge(sulfuric acid)Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)(Alumina Balls for Hydogen Peroxide, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, flow rate of 0.1 mL/min., and l = 620 nm.

Magic Tape Hook and Loop Fasteners.Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fibershows the peptides separations after enzymatic hydrolysis.Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPen Type pH Meter pH-031&0.00-14.00pH Tester, but not shown inStamping Metal Parts Manufacturer Sheet Parts Fabrication,Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), and also free amino acids (Whitaker et al., 1963).25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Alumina Balls for Hydogen Peroxide,1996).

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Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, V_e, and K_avcalculated as discussed previously.Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price,Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60) (Reis et al.,1989;Garlic Red Mesh Bag.,1991;Anti-High Pressure Insulated Pliers with PVC Dipped Handles.,1991).Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments(1992), were lower, except for cystine and tyrosine,Factory Supply Cheap Custom Bleached White Cotton Gloves.

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CONCLUSIONS

2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory) for the financial support.

REFERENCES

304 &314 Stainless Steel Seamless Bending Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydraulic Hose And Connection]

Brown, E. M; Thompson, C .J.,Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, M.M.(1994),Custom Grey Full-Zip Cotton Mens Hoodies.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pond Self Priming Booster suction  Water Pump with Pressure Switch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flexible Magnetic Rubber Sheet Soft Magnetic Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. Analytical Biochemistry212,436-445[Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine]

Dalev, P.G. and Simeonova, L.S.(1992),7inch Industrial Tablet Computer Naked Resistance Touch Screen. Biotechnology letters 14,6, 531-534[Ep/Nn/EPP Industrial Rubber Conveyor Belt]

Heidemann, E.(1997),Glass Door Passenger Sightseeing Elevator for Sale.Leder 48,(5),99-104[Double Gear]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100%Polyester Satin Fabric for Blouse]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lantern for Garden Decoration]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Energy Saving Street Light E27 E40 27W LED Corn Bulb]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fixed Competitive Price Fly Fishing Boat Bag - White black pure string hunting camouflage ghillie suit - Demarui]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Multi-Function Small Tree Digging Shovel/Tree Machine]

Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fcy30 Transport Mini Tipping Dumper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Stainless Steel Double Small Torsion Spring]

Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1 Ton Chain Pulley Block with Geared Trolley]

Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, Part I.Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Spooling Pet Polyester Mylar Film for Cable Material]

Premium Grade Rubber Cloth Duct Tape, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sud1000h Poly Welder Butt Fusion Machine.Customized Anti-Rust Torsion Spring, 175-184[Cole And Bright Solar Sensor Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Micro-Combustion Tube]

Whitaker,J.R.(1963),Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining. Analytical Chemistry35(12), 1950-1953[Chemical Product Sodium Gluconate Used for Concrete Admixture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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