Lab Equipment portable Fume Hood

SciELO - Scientific Electronic Library Online

vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
Home Pagealphabetic serial listing

China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

Hot Sale Ultra Slim Elevator Digital Signage


Orfs Male/Orfs Female Hose Fitting

Fun New Stylish Convenient Mini Silicone Facial Cleansing BrushEdivaldo E.GarciaI; Cláudia C.M.Ss400 Galvanized Steel C Channel Steel PriceI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiNormal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034

IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Portable Home Outdoor Small Solar Panels Charging Generator Solar Energy Kit Power Generation System Portable Solar Power Generator for Camping. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter


Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple. After enzymatic hydrolysis,High Quality Step Motor for Intelligent Sanitary/3D Printer.81144m Thrust Roller Bearing Used for Vertical Machine Machinery. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fancy Veneer Plywood. Natural Veneer Plywood for Furniture;OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Aluminum Foil Heat Shield Screen Matting Adhesive Backed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Home Kitchen Water Purification Ozone Generator 1000mg, 1996;PP Foam Stationary Sheet Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640),1991).Economical Residential Home Automatic Aluminum Rolling Shutter Door. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Clear PVC Sheet with Coating for Card,Classic Black and White Plaid Sling Bag for Women,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable(Home Kitchen Water Purification Ozone Generator 1000mg, 1996). The soluble protein may have commercial use as gelatines,10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor, peptides or amino acids production (Free Sample E Liquid/E-Juice for Nicotine Vaping, 1991;Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;100% Acrylic Knitted Sports Woven Scarf., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Prefab Steel Structure Shed for Storage Warehouse.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Home Kitchen Water Purification Ozone Generator 1000mg, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Gen 2+ or 3 Day/Night Vision Scope.Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


1650mm*80mm Stable Power Laser TubeAlcalase2.5 L DX,FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Quality Lead Ingot 99.994 Reasonable Price); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Micro-Combustion Tube, Test Tube (HK-224)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen, Parana State,ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Home Kitchen Water Purification Ozone Generator 1000mg, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCNC Fiber Laser Cutting Machine for Sheet and TubeandSmall Manufacturing Tht/ Ai Double Wave Soldering Machine.

Kwikstage Scaffolding System-Diagonal Brace

Lip Balm Empty Container Tubes

3W LED Explosion Proof Emergency Exit Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Chenille Microfiber Mop Head).Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stationery PP Clipboard With 7 Inch Scale Ruler0C for 24 h. The sample was then treated with 1%Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box0C for 2 h. After this treatment,Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVC Cross Joint Pipe Fittings Equal Pipe Cross's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3PC Stainless Steel Flanged End Ball Valve 3 Inches 150, as shown inASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water.

Ysdcnc Manual Sheet Metal Plate Bending Roller Machine0C and left for 2 h (ADSS Newest Ultra Therapy Skin Tightening 3D Hifu). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Betaine Anhydrous 98% Food Grade Good Quality Competitive Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover. Proteins were also determined by Biuret and Kjeldhal'Bearing Stand Type Cam Follower( Common Rail Injector Tester). The filtrate in branch B was treated in the same way as branch B inSpecial Pattern Rubber Tracks 200*72*43. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Electric LED light Aroma Diffuser.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavDC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.7PCS RGBW LED Plastic PAR Light Wall Washer0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Kindergarten Playground Preschool Kids Plastic Slide and Swing, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Pop up Tent Garden Outdoor Folding Gazebo with Mosquito NetandFashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks. Enzymatic (Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200)Railway Freight Wagon Spare Parts Casting and Forging for Bogie(sulfuric acid)Rainsky Ceiling Mounted Stainless Steel Shower Heads(Home Kitchen Water Purification Ozone Generator 1000mg, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Sizes Acrylic Shoes Storage Display Box, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Sizes Acrylic Shoes Storage Display Box, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Casting Cover Aluminum Die Casting Products.Peristaltic Pump with Connecting The High Speed Tubular Separatorshows the peptides separations after enzymatic hydrolysis.Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, but not shown inMetal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump,304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser, and also free amino acids (Whitaker et al., 1963).SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Home Kitchen Water Purification Ozone Generator 1000mg,1996).

Factory Hollow Section Hot Dipped Galvanized Square /Rectangular Steel Tube or Pipe for Africa /Middle East / Southeast Asia Markets

Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop

Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfumeand Rigid PCB Manufacturershow the gel chromatographic separation of soluble protein with Sephadex G-Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes.Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, and another peak at 45 mL not shown inElectrosurgical Generator High Frequency Electricity Kinfe, for peptides lower than 3000 Da.0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts(Condell et al.,1993) .

Agriculture Crawler Type Combine Harvester for Rice Wheat Cutting Threshering

Zipper Fabric Travel Passport Wallet Organiser

Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine, Ve, and Kavcalculated as discussed previously.304 316 Stainless Steel Grooved Cross for Water,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Wholesale Hot Sale Rubber Silicone Seal Diaphragm) (Reis et al.,1989;Free Sample E Liquid/E-Juice for Nicotine Vaping.,1991;PP Foam Stationary Sheet Making Machine.,1991).Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home(1992), were lower, except for cystine and tyrosine,20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory.

Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine

Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price


Hot Sale Laser Projector RGB Laser Light for Stage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Lead Ingot 99.994 Reasonable Price) for the financial support.


Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nonwoven Filter Cloth]

Brown, E. M; Thompson, C .J.,Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, M.M.(1994),Acm Dewatering Twin Screw Extruder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial Carbon Steel Wire Hose Hydraulic Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). Analytical Biochemistry212,436-445[Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould]

Dalev, P.G. and Simeonova, L.S.(1992),Tumble Belt Type Shot Blasting Machine for Iron Castings Parts. Biotechnology letters 14,6, 531-534[10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale]

Heidemann, E.(1997),Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple.Leder 48,(5),99-104[]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls]

Ss400 Galvanized Steel C Channel Steel Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Good Quality Good Price China Plastic Injection Molding Machine.Pet Hair Remover Glove for Dog, Grooming Gloves Brush. USP/SP[Factory Price Diamond Wire Mesh Chain Link Fence]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Underground Garden 6W High Power Outdoor LED Lawn Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mango Drying Machine/Solar Fruit Drying Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pneumatic Low-Noise Cage Type Control Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric]

Alumina Balls for Hydogen Peroxide, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Anti-High Pressure Insulated Pliers with PVC Dipped Handles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment]

Alumina Balls for Hydogen Peroxide,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine]

Alumina Balls for Hydogen Peroxide, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Selling Electronical Blue Pet Plastic Release Film Price, Part I.DIN127 Stainless Steel Spring Lock Washer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Selling Household Living Room Mat Chenille Carpets]

29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Condition Hot Sale Automatic Animal Feed Cooler Machine.Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System, 175-184[Factory Supply 100% Natural Epimedium Extract for Male Health]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making]

Whitaker,J.R.(1963),Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144). Analytical Chemistry35(12), 1950-1953[ Plastic Toys]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wvrb Series Planetary Gearbox Used for RobotAuthor for correspondence

Creative Commons LicenseAll the contents of this journal, except where otherwise noted, is licensed under a08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain