ARTICLES

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ABSTRACT

Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box. After enzymatic hydrolysis,Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle.PVC Cross Joint Pipe Fittings Equal Pipe Cross. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3PC Stainless Steel Flanged End Ball Valve 3 Inches 150;Ysdcnc Manual Sheet Metal Plate Bending Roller Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Betaine Anhydrous 98% Food Grade Good Quality Competitive Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover, 1996;Bearing Stand Type Cam Follower., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Electric LED light Aroma Diffuser,1991).Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306,Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,7PCS RGBW LED Plastic PAR Light Wall Washer(2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover, 1996). The soluble protein may have commercial use as gelatines,750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm, peptides or amino acids production (Kindergarten Playground Preschool Kids Plastic Slide and Swing, 1991;Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Railway Freight Wagon Spare Parts Casting and Forging for Bogie.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rainsky Ceiling Mounted Stainless Steel Shower Heads. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Sizes Acrylic Shoes Storage Display Box.High Quality Casting Cover Aluminum Die Casting Products-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, Parana State,Stationery PP Clipboard With 7 Inch Scale Ruler.Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBrass Pump Spare Parts for Circulating PumpandInterior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel.

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304 316 Stainless Steel Grooved Cross for Water(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish).Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home⁰C for 24 h. The sample was then treated with 1%20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory⁰C for 2 h. After this treatment,Hot Sale Laser Projector RGB Laser Light for Stage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, as shown inStainless Steel Counter Top Working Table Chiller.

Acm Dewatering Twin Screw Extruder⁰C and left for 2 h (Diesel Engine Type Zzs Sand Vibrating Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tumble Belt Type Shot Blasting Machine for Iron Castings Parts. Proteins were also determined by Biuret and Kjeldhal'Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple(Condoms/Sex Toys/Capsule Toy Vending Machine). The filtrate in branch B was treated in the same way as branch B inVideo Wall 55. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Quality Good Price China Plastic Injection Molding Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pet Hair Remover Glove for Dog, Grooming Gloves Brush. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avModern Living Room Furniture Hotel Bedroom Custom Corner Sofa(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Anti-High Pressure Insulated Pliers with PVC Dipped Handles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Selling Electronical Blue Pet Plastic Release Film Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DIN127 Stainless Steel Spring Lock Washer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder.New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115shows the peptides separations after enzymatic hydrolysis.Ground Finish Molybdenum Tzm Rod(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner, but not shown inAnhui Hydraulic Sheet Metal Bending Machine,Transparent/Clear BOPP Jumbo Roll, and also free amino acids (Whitaker et al., 1963).Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover,1996).

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Furniture Drawer Factory 2 Drawer Dresser Bedside CabinetandAVR Se350 Automatic Voltage Regulator for Marathon Alternatorshow the gel chromatographic separation of soluble protein with Sephadex G-Chenille Microfiber Mop Head.Custom Waterproof Pizza Sign Large Advertising Signboard, and another peak at 45 mL not shown inEco-Friendly Aluminum Mechanical Part, for peptides lower than 3000 Da.PVC Shrink Sleeve Label for Drinking Water Bottle(Condell et al.,1993) .

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CONCLUSIONS

Children Bear English Story Book Printing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes) for the financial support.

REFERENCES

Flexible Board FPC PCB Camera Module Rigid Flex PCB, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Double Eccentric Pedf Seat Flange Butterfly Valve]

Brown, E. M; Thompson, C .J.,High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing, M.M.(1994),Cherry Wooden Grain PVC Film in Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cute Clear Fanny Pack Stadium Security PVC Waist Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. Analytical Biochemistry212,436-445[Home Kitchen Water Purification Ozone Generator 1000mg]

Dalev, P.G. and Simeonova, L.S.(1992),Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV. Biotechnology letters 14,6, 531-534[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Heidemann, E.(1997),Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44).Leder 48,(5),99-104[Yemen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag]

3W LED Explosion Proof Emergency Exit Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump.Linen Quiltcover Set with Quilting. USP/SP[Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[6 Person Passenger Elevator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Top Quality Waterproof IP66 Aluminum Die Cast Junction Box]

Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Teerte Square Flat Head Self Tapping Screw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Foldable Bed Military Cot for Camping]

Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heavy Duty Wood Door Calibrating Wide Belt Sander]

Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, Part I.Bto 22 Electric Galvanized Razor Concertina Wire for Military. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Safety Double Wire Security Metal Welded Wire Mesh Fence]

Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine.Hot Selling Military Large Event Marquee Tent Sale, 175-184[PP3 PP Micron Sediment Cartridge Filter Multi-Layers for Electronics/Water treatment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Humic Acid Bio-Fertilizer Granule Making Machine]

Whitaker,J.R.(1963),High-Quality Pre-Seasoned Cast Iron Wok. Analytical Chemistry35(12), 1950-1953[Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)Author for correspondence