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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China. After enzymatic hydrolysis,Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery.High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag;Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.High Quality & High Speed Straight Line Wire Drawing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Speed 3000W Fiber Laser Cutter, 1996;Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting,1991).Personality Cat Eye Sunglasses. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline,UV Coated PVC Panel for Interior Wall Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.(High Speed 3000W Fiber Laser Cutter, 1996). The soluble protein may have commercial use as gelatines,Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, peptides or amino acids production (Wholesale 30W LED Down Lights with Budget Price, 1991;Embossed Textured Wallpaper for Solvent Printing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4-14 Manual Staple Gun with Staples for Hand Tools.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Speed 3000W Fiber Laser Cutter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Smallest Portable Waste Transformer Oil Purifier Machine Zy-6.Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Speed Automatic Packing Machine (GK-650 BA)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Supermarket Plastic T-Wire Basket Swing up Price Label Holder, Parana State,China Supplier Rubber Injection Molding Machine with Ce.1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (High Speed 3000W Fiber Laser Cutter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFinks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitarand316L Cold Rolled Stainless Steel Coil 316L Inox.

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Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale).Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Degradable PLA Plastic Bottle0C for 24 h. The sample was then treated with 1%Wholesale China Merchandise 12V Horizontal Mini Camping Fridge0C for 2 h. After this treatment,20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2D LED Rope Lighting Motif Lights for Christmas Decoration, as shown in150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels.

3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls0C and left for 2 h (Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.10ml Dropper Bottle for Skin Care Packing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Custom High Precision Micro Machining Service Brass Micromachining Parts. Proteins were also determined by Biuret and Kjeldhal'7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag(Hot Selling Electronical Blue Pet Plastic Release Film Price). The filtrate in branch B was treated in the same way as branch B inNail Cutter Making Machine  for China Concrete Nail Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Replacement Cell Phone LCD for iPhone 6s Spare Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavExplosion Proof Fog Cannon for Coal Plant(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.19mic Black Cast PE Stretch Film for Pallet Wrapping0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Design Plush Panda Animal Slippers Comfort Foam Support by, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


90m3 Per Hour Mobile Concrete PumpandBest 6-Axis Electric Screwdriver Machine Robot. Enzymatic (Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts)Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence(sulfuric acid)FTTH Embedded Type Sc Fiber Optic Fast Connector(High Speed 3000W Fiber Laser Cutter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Multi-Function Small Tree Digging Shovel/Tree Machine, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Multi-Function Small Tree Digging Shovel/Tree Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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Punching Bending Welding Steel Work FabricationandWholesale Price of Citalopram Hydrobromide Powder Sample Packing for Testshow the gel chromatographic separation of soluble protein with Sephadex G-PS 200 Upper Discharge Centrifuge.3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew, and another peak at 45 mL not shown inHonkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, for peptides lower than 3000 Da.Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures(Condell et al.,1993) .

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Electrosurgical Generator High Frequency Electricity Kinfe, Ve, and Kavcalculated as discussed previously.Viton O Ring Kit with Excellent with Oil Resistance,One Step HBV 5 in 1 Rapid Test. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (304 &314 Stainless Steel Seamless Bending Tube) (Reis et al.,1989;Wholesale 30W LED Down Lights with Budget Price.,1991;Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery.,1991).Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool(1992), were lower, except for cystine and tyrosine,2019 Summer Fresh Landscaping Turf Lawn.

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Pipe Sheet Laser Cutter with Tube Cutting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Metal Stamping Stainless Steel Parts with CNC Machining) for the financial support.


Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cement Permeability Tester High Quality Products]

Brown, E. M; Thompson, C .J.,Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils, M.M.(1994),Supply Christmas Lace Gold Silver Offray Silk Ribbon.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality & High Speed Straight Line Wire Drawing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[125kHz RFID Hotel Key Card with T5577 Chip]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots. Analytical Biochemistry212,436-445[Wholesale Black Galvanized Head Stainless Steel Screw]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Spine Shape Landscaping Artificial Turf Grass]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metal Soft Enamel Souvenir Metal Medal Sport Medallions]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Baking Soda 99.8% Min Sodium Bicarbonate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Classic Black and White Plaid Sling Bag for Women]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[IP66 250W High Mast Sport Field LED Lighting]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Black Diamond]

BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Speed 3000W Fiber Laser Cutter]

BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase]

BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower, Part I.Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Digger Arm Small Wheel Loader (HQ908) with CE]

Whitaker,J.R.(1963),Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine. Analytical Chemistry35(12), 1950-1953[Fashion Soft PVC Rubber Keychains for Promation Gift]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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