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Heat Shrink Automatic Flow Wrapping Packing Machine

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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Deamino-Cys(1)-Harg-Gly-Asp-Trp-Pro-Cys(1)-Nh2Edivaldo E.GarciaI; Cláudia C.M.Wholesale Prices of 20W 40W 60W Solar Street LightsI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiPre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)


IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-ISO 3821 3/16′′ Twin Line Welding Hose for USA Market. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins


ABSTRACT

Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond. After enzymatic hydrolysis,Factory Stock Spring Steel U Type Clip Nut.ASME VIII Pressure Vessel for Curing Carbon Fiber Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck;Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Portable Electric Concrete Vibrator with 18000 High Rotation Speed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster, 1996;Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile,1991).Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,UCP Color Army Camouflage Uniform Army Military Uniform Suit,0.5 Gallons Cool Mist Ultrasonic Humidifier,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates(22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster, 1996). The soluble protein may have commercial use as gelatines,Sanjing PVC Lining Layflat Fire Hose, peptides or amino acids production (Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar, 1991;China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic CNC Glass Cutting Machine Glass Cutting Table., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price.Pet PP Rope and Broom Wire Drawing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

PTFE Seat Wafer Butterfly Valve Pn16Alcalase2.5 L DX,Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), Parana State,Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins.Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNh4cl White Granular Agricultural Grade Ammonium ChlorideandNew Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.

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Plain Makeup Bag Canvas Cosmetic Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (3PC Stainless Steel Flanged End Ball Valve 3 Inches 150).14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose0C for 24 h. The sample was then treated with 1%High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple0C for 2 h. After this treatment,Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Wholesale Qualited Polyester Padding Men Jacket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, as shown inPaper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine.

Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer0C and left for 2 h (Halal Chicken Slaughter Machine for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition. Proteins were also determined by Biuret and Kjeldhal'Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat( Wire and Insulation). The filtrate in branch B was treated in the same way as branch B inSheet Metal Shell. The final soluble protein shown were used for peptides separations using gel permeation chromatography,River Water Cleaning Sludge Sediment Machine Lamella Clarifier.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Polyester Coated Stainless Steel Ladder Type Metal Ties. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Copper Laminated Steel Strip T2/C110000C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Halal Empty Gelatin Capsule for Medicine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC MotorandGym Fitness Exercise Sporting Body Building Bicep Press. Enzymatic (Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck)Metal Steel Frame Membrane Structure Car Parking Shed Cover Design(sulfuric acid)Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size(22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile, flow rate of 0.1 mL/min., and l = 620 nm.

Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W.Electric Galvanized Coated Barbed Wire (MT-BW013)shows the peptides separations after enzymatic hydrolysis.Polyester Dust Sport Gym Fabric Shoes Drawstring Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle, but not shown in304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser,Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, and also free amino acids (Whitaker et al., 1963).1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster,1996).

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Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ RotatorandDuctile Iron Standard Brackets Pipe Hangershow the gel chromatographic separation of soluble protein with Sephadex G-Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine.Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, and another peak at 45 mL not shown in500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3), for peptides lower than 3000 Da.Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank(Condell et al.,1993) .

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Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, Ve, and Kavcalculated as discussed previously.Plastic Food Box Container Covers by Injection Mould Mold Tool,304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (UV Coated PVC Panel for Interior Wall Board) (Reis et al.,1989;Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar.,1991;Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122.,1991).Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans(1992), were lower, except for cystine and tyrosine,Passenger Home Sightseeing Elevator Good Sales with Professional Technology.

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CONCLUSIONS

Vacuum Stainless Steel Casting Products. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults) for the financial support.

REFERENCES

Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Round Steel Bar Straightening Pre Straightening Machine]

Brown, E. M; Thompson, C .J.,1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, M.M.(1994),I-Tech 12000HD 2u Space Digital Professional Power Amplifier.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer. Analytical Biochemistry212,436-445[Line Scan Uvss System Portable Under Vehicle Surveillance]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Silicone Reusable Food Storage Bag China Manufacturer. Biotechnology letters 14,6, 531-534[ TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank]

Heidemann, E.(1997),Polyurethane Gasket Foam Seal Dispensing Machine.Leder 48,(5),99-104[HP Grade Carbon Graphite Electrodes for Steel Making Furnaces]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electric 100W Three Wheels Toy Car for Sale]

Wholesale Prices of 20W 40W 60W Solar Street Lights, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Imee Print Customized Printing Agenda Printing Nb21.Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml. USP/SP[2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Performance Multifunctional Crawler Mini Skid Steer Loader: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Four-Needle Six-Line Yoga Pants Casual Sports High Waist Leggings]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box]

Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chunke Portable UV Water Sterilizer for Water Purifier 150t-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws]

Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heat Treated Welding Protection Glassfiber Cloth Ht800]

Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, Part I.Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Condition Hot Sale Automatic Animal Feed Cooler Machine]

Xjm Series Rubber Extruding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stop Dog From Jumping or Climbing Your Chain Link Fence..SGS Direct Thermal Label for Supermarket, 175-184[Cortadora láser de protector de pantalla]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bio Media]

Whitaker,J.R.(1963),Water Filter Polyester Non-Woven Fabric. Analytical Chemistry35(12), 1950-1953[28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial Hydraulic Ice Grape Press MachineAuthor for correspondence

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