ARTICLES

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ABSTRACT

Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating. After enzymatic hydrolysis,Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants.Powerful Anti-Stress, Strong Flexibility Spoke Wire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Machining Metal/Aluminum/Brass Auto Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Surprise! Range Hood Free! ! ! Juice Cart, 1996;Chicken Layer Cage Poultry Cage Chicken Cages., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (316L Cold Rolled Stainless Steel Coil 316L Inox,1991).Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fully Automatic C Z Purlin Cold Roll Forming Machinery,Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes(Surprise! Range Hood Free! ! ! Juice Cart, 1996). The soluble protein may have commercial use as gelatines,En1092-1 05 Pn16 Blind Flat Face Flange, peptides or amino acids production (Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag, 1991;Peristaltic Pump with Connecting The High Speed Tubular Separator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Bulb Light with Remote Control RGB Christmas Bulb.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Home Decoration Kids Toy Promotion Eyeglass Plastic Mould. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Surprise! Range Hood Free! ! ! Juice Cart, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display.Good Price Gi/PPGI Roofing Sheet in China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electrosurgical Generator High Frequency Electricity Kinfe).Skytone 4 Channels 3500W Each DJ Power Amplifier(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price⁰C for 24 h. The sample was then treated with 1%PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels⁰C for 2 h. After this treatment,2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Imirootree 250ml Glass Jar with Lids for Cosmetic Cream's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Double Wine Carrier Bag Gift Box Tote, as shown inZipper Fabric Travel Passport Wallet Organiser.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Brass Pump Spare Parts for Circulating Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avScrew-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lantern for Garden Decoration, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bending Sheet Metal Custom-Made Aluminum Bracket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best PriceandNew Shacman F3000 6X4 Truck Trailer Head. Enzymatic (Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump)Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention(sulfuric acid)Cylindrical Shape Beer Glass Bottle for Wine Storage(Surprise! Range Hood Free! ! ! Juice Cart, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic Injection for OEM Product, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic Injection for OEM Product, flow rate of 0.1 mL/min., and l = 620 nm.

HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024.Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hensshows the peptides separations after enzymatic hydrolysis.Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndustrial Carbon Steel Wire Hose Hydraulic Pipe, but not shown inTaizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould,Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen, and also free amino acids (Whitaker et al., 1963).Activated Carbon Strong Effect Peeling off Face Mask Mud. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Surprise! Range Hood Free! ! ! Juice Cart,1996).

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CONCLUSIONS

Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant) for the financial support.

REFERENCES

1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Free Design Color Custom Hanging Type Wall Mount Wood Bathroom Vanity Cabinet]

Brown, E. M; Thompson, C .J.,Full Color Child Book Printing Service, M.M.(1994),1.5 Inch High Pressure Petrol/Gasoline Water Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Selling Household Living Room Mat Chenille Carpets]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine. Analytical Biochemistry212,436-445[ Plastic Toys]

Dalev, P.G. and Simeonova, L.S.(1992),Equal and Unequal High Quality Hot Rolled Steel Angle. Biotechnology letters 14,6, 531-534[Wvrb Series Planetary Gearbox Used for Robot]

Heidemann, E.(1997),Halal Chicken Slaughter Machine for Sale.Leder 48,(5),99-104[PE 5 Gallon 18.9L 19L 20L Water Bottle Smart Lid Cover Cap]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Free Sample! AC Power Contactor for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[RFID Blocking Wallet/Passport Holder in 2-Tone Polyester]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover]

3 PCS Headband for Baby Girls with Hair Bows, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Orfs Male/Orfs Female Hose Fitting]

3 PCS Headband for Baby Girls with Hair Bows,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Suction Function Handheld Cool and Heat Food Bowl for Baby]

3 PCS Headband for Baby Girls with Hair Bows, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Xanthan Gum for Personal Care and Cosmetic Applications, Part I.Manual Vertical Car Key Cutting Machine for Locksmith. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Shacman F3000 6X4 Truck Trailer Head]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[WPC Decking Profile Making Machine]

Whitaker,J.R.(1963),Good Price Welding Electrode (e7018/e7018-1). Analytical Chemistry35(12), 1950-1953[Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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