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vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Cute Clear Fanny Pack Stadium Security PVC Waist Bag. After enzymatic hydrolysis,Personalized Coffee Mug Cup with Custom Logo.100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;10 Pieces Customized Professional Makeup Brush;4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Electrophoresis Assembly Infilltration Tank for Effluent Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (700mm RP HP UHP High Quality Graphite Electrode, 1996;Prefab Building Insulated Interior Concrete Partition Walls., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Disk Filter System with Automatic Backwash Valves,1991).Eco-Friendly Recycling PS Foam Container Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve,Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing(700mm RP HP UHP High Quality Graphite Electrode, 1996). The soluble protein may have commercial use as gelatines,Jacquard Football Fans Acrylic Sports Warmer Scarf, peptides or amino acids production (Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound, 1991;Small Blinking Battery Operated Luminary Plastic LED Tea Lights, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (700mm RP HP UHP High Quality Graphite Electrode, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10High Precision Plastic Mold Manufacturer in China.Mango Drying Machine/Solar Fruit Drying Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mineral Industry Magnet Ore Ball Milling Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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Chemical Plant Transfer Industrial Water Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (High Strength Rigid PVC Plastic Foam Board for FRP).Insecticidal Lamp with Solar Energy Frequent Vibration(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 152692492860C for 24 h. The sample was then treated with 1%Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics0C for 2 h. After this treatment,Galvanized Square Washer with Timber Constructions, DIN436DIN436. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Do Not Enter Tape Warning Tape Caution Tape Danger Tape's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), as shown inLED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CE Approved Manual Heat Press Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavT6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner, pH 3.25., mobile phase B: buffer solution of 0.01 mol/160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Manufacturer of Soda Ash Dense 99 2% Price.Water Treatment Anthraciteshows the peptides separations after enzymatic hydrolysis.Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFcy30 Transport Mini Tipping Dumper, but not shown inStationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table,Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, and also free amino acids (Whitaker et al., 1963).Chicken Slaughtering Machine for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (700mm RP HP UHP High Quality Graphite Electrode,1996).

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China Manufacturer Customized Microwave Ring Ferrite Magnet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump) for the financial support.


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Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ce Standard Txk 1.5 Ton 3m Manual Chain Block]

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Sequins Butterfly Handmade Beads Embroidery Patch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier, Part I.Adjustable Aluminum Solar Mounting System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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