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ABSTRACT

304 &314 Stainless Steel Seamless Bending Tube. After enzymatic hydrolysis,Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark.Custom Grey Full-Zip Cotton Mens Hoodies. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Glass Door Passenger Sightseeing Elevator for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, 1996;12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple,1991).Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fcy30 Transport Mini Tipping Dumper,Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)(Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, 1996). The soluble protein may have commercial use as gelatines,Premium Grade Rubber Cloth Duct Tape, peptides or amino acids production (Sud1000h Poly Welder Butt Fusion Machine, 1991;Customized Anti-Rust Torsion Spring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic.PP Woven Bag, Rice, Beans, Plastic Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Professional Mold Factory Plastic Injection Mould Maker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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50mm Magnetic River Water Sewage Flow Meter for Acid(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies).PE Biodegradable Sheet Product Line/Single Screw Extruder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%27L/Min Water Exchanger Heat Pump Mold Temperature Machine⁰C for 24 h. The sample was then treated with 1%Felt Belt for Textile Compactor⁰C for 2 h. After this treatment,Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Metal Soft Enamel Souvenir Metal Medal Sport Medallions's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)20L Water Tank Lid Mold 3D Printing Samples Available, as shown inReplacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale.

Wholesale High Quality Metal Car Mount Holder Phone Holder⁰C and left for 2 h (27L/Min Water Exchanger Heat Pump Mold Temperature Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns. Proteins were also determined by Biuret and Kjeldhal'Pet Laminate Aluminium Foil/Pet+Alu+PE(Custom All Kinds of Acrylic Shoe Display Racks). The filtrate in branch B was treated in the same way as branch B inHgy33m Automatic Concrete Boom Placer Self-Climbing Concrete Placing Boom. The final soluble protein shown were used for peptides separations using gel permeation chromatography,6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Design Multi-Color Silicone Coin Purse for Lady. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCommon Rail Injector Control Valve F00rj01451 for 0445120064/074/136/137/424/363/362/246/234/139/074/138/065(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dark Green Plastic PVC Film for Christmas Tree Leaves⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Disposable Non-Woven Dry Gloves Wipes Making MachineandFurniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nutshow the gel chromatographic separation of soluble protein with Sephadex G-Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator.Injector Pump Calibration Test Bench, and another peak at 45 mL not shown inRotary Vibrating Sieve Machine for Compost, for peptides lower than 3000 Da.ASME/ASTM Butt-Welding Stainless Steel Plate Flange(Condell et al.,1993) .

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CONCLUSIONS

Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial Carbon Steel Wire Hose Hydraulic Pipe) for the financial support.

REFERENCES

The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Moving Tape Heavy Duty Adhesive Tape]

Brown, E. M; Thompson, C .J.,Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, M.M.(1994),Wholesale All Kinds Cheap Plush Animal Baby Slippers.The Journal of the American Leather Chemists Association (JALCA)89(7)[Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Do Not Enter Tape Warning Tape Caution Tape Danger Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car. Analytical Biochemistry212,436-445[Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner]

Dalev, P.G. and Simeonova, L.S.(1992),Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane. Biotechnology letters 14,6, 531-534[Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine]

Heidemann, E.(1997),Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine.Leder 48,(5),99-104[Toppling Tower]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr]

Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress.Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale. USP/SP[Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Taiding Custom Tank Sheet Metal Fabrication: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Powder Coated H Column Beam Steel Structure Building for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless 1 Inch Welded Steel Pipe Sch10 Sch40]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Water Based Emulsion Paint Production Line]

Airtight Candy Bin with Scoop, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8]

Airtight Candy Bin with Scoop,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Different Thickness High Quality Aluminum Foil for Glass Bottle Lid]

Airtight Candy Bin with Scoop, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell, Part I.3 Layer Round Tea Tin with Air Tight Lid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Supplier Building Construction Steel Frame (ZY431)]

China PA System Power Amplifier with Enough Rated Power, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Export Grade Citric Acid Monohydrate as Detergent.Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace, 175-184[CNC Machined Stainless Steel Part]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sc-2400 Automatic Multi Function Sosn Side Drilling Machine]

Whitaker,J.R.(1963),Familu Use Mini Small Pig Feed Pellet Mill. Analytical Chemistry35(12), 1950-1953[Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe PumpAuthor for correspondence