ARTICLES

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ABSTRACT

Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow. After enzymatic hydrolysis,Wheel-Type-Carriage Precision Heavy Duty Linear Guide.Double-Offset Double Flanged Stainless Steel Butterfly Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automotive Acrylic Wholesale Masking Tape Factory Price;Home Decoration Candle Glass Holder for Table Decorative; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Black Galvanized Head Stainless Steel Screw, 1996;Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair,1991).OEM Logo Soft Silicone Reusable BPA Free Drinking Straws. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin,4 Drawer Steel Office Vertical Filing Cabinet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car(Wholesale Black Galvanized Head Stainless Steel Screw, 1996). The soluble protein may have commercial use as gelatines,Parts for Crane Outrigger Hydraulic Oil Cylinder, peptides or amino acids production (Boned Polyester Woven Faux Suede Sofa Fabric, 1991;Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20 Bottles Electric Wine Display Cooler., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High-Efficient Htk Walnut Shell Filter for Water Purification.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Black Galvanized Head Stainless Steel Screw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100).Popular European Style White Marble Coffee Tables for Dinnig Room-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, Parana State,Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal.IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Black Galvanized Head Stainless Steel Screw, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDongfeng 153 Type 10m3 12m3 Arm Roll Garbage TruckandSkytone 4 Channels 3500W Each DJ Power Amplifier.

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Sell Rubber / EVA / PE/PVC Foam Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic L Bar Sealer Shrink Tunnel Machine).Sodium Gluconate 98% Min Industry Grade for Chemical Admixture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Brake Disc⁰C for 24 h. The sample was then treated with 1%DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign⁰C for 2 h. After this treatment,24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder, as shown inCommon Rail Diesel Injector Nozzle Dlla153p977.

LED Screen Display Wireless Charging 10000mAh Power Bank⁰C and left for 2 h (Polyurethane Gasket Foam Seal Dispensing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.25kg 50kg Plastic PP Woven Feed Bags. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,360 Degree Rotation Medical Seat Cushion. Proteins were also determined by Biuret and Kjeldhal'Custom LCD Screen Video Box for Package(Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair). The filtrate in branch B was treated in the same way as branch B inChina Manufacturer Custom Plastic Molding for Plastic Molded Connector. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Size Christmas Gift Personalized Watch Boxes for Men, pH 3.25., mobile phase B: buffer solution of 0.01 mol/12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Our Perforated Aluminum & Solid Panel for New Office Wall Cladding.ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Ventingshows the peptides separations after enzymatic hydrolysis.2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Ice Cube Maker Ice Making Machine with Selectable Cube Size, but not shown inCleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board,Kids Trucker Hat, Kids Mesh Cap, and also free amino acids (Whitaker et al., 1963).Wd190V Handheld Full Body Scanner Hand Held Metal Detector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Black Galvanized Head Stainless Steel Screw,1996).

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CONCLUSIONS

ISO 3821 3/16′′ Twin Line Welding Hose for USA Market. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (360 Rotating Automatic Touchless Car Wash Equipment with Drying System) for the financial support.

REFERENCES

Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mirror Frame]

Brown, E. M; Thompson, C .J.,Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond, M.M.(1994),Factory Stock Spring Steel U Type Clip Nut.The Journal of the American Leather Chemists Association (JALCA)89(7)[DIN127 Stainless Steel Spring Lock Washer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ASME VIII Pressure Vessel for Curing Carbon Fiber Parts. Analytical Biochemistry212,436-445[Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM]

Dalev, P.G. and Simeonova, L.S.(1992),Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck. Biotechnology letters 14,6, 531-534[Wet Wire Drawing Machine for High Carbon Mattress Spring Wire]

Heidemann, E.(1997),Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut.Leder 48,(5),99-104[Rotary Seal Rotary Joint Steel Oil Rotary Union]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Valve Bag Dry Mortar Bagging Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Caustic Soda And Soda Ash]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car]

Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone]

Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai]

Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:UCP Color Army Camouflage Uniform Army Military Uniform Suit, Part I.0.5 Gallons Cool Mist Ultrasonic Humidifier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale]

Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sanjing PVC Lining Layflat Fire Hose.Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar, 175-184[Ductile Iron Pipe Repair Clamp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

Whitaker,J.R.(1963),China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light. Analytical Chemistry35(12), 1950-1953[Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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