ARTICLES

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ABSTRACT

Cantilever Oxygen Plasma Cutter 80. After enzymatic hydrolysis,Stainless Steel Expansion Anchor Bolt Steel Zinc Plated.CNC Fiber Laser Cutting Machine for Sheet and Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval, 1996;High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper,1991).300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,BS 5163 Ductile Iron Rising Stem Gate Valve,CNC Aluminum Sheet Press Brake,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ID25mm Od86mm Standard Through Hole Slip Ring(Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval, 1996). The soluble protein may have commercial use as gelatines,Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, peptides or amino acids production (Bio Media, 1991;Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Platform Trolley with 150kg Capacity, 4 Castor Wheel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰All Size Colored Tapping Hex Head Self Drill Screw Wood Screw.Pre-Engineered Cage Broiler Poultry Farm Building-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Alumina Ball with High Quality(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hero Brand Polypropylene Bags Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper).CNC Machining Metal/Aluminum/Brass Auto Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Surprise! Range Hood Free! ! ! Juice Cart⁰C for 24 h. The sample was then treated with 1%Chicken Layer Cage Poultry Cage Chicken Cages⁰C for 2 h. After this treatment,316L Cold Rolled Stainless Steel Coil 316L Inox. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fully Automatic C Z Purlin Cold Roll Forming Machinery, as shown inSteel Bar Peeling Lathe Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED Bulb Light with Remote Control RGB Christmas Bulb(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Price Gi/PPGI Roofing Sheet in China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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M4X10 Round Head Core Pulling Aluminum Nail.Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truckshows the peptides separations after enzymatic hydrolysis.Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPearls Flake 99% Factory Naoh Caustic Soda, but not shown inLinear Guideway Flat Bed CNC Lathe Machine (BL-Z0640),Skytone 4 Channels 3500W Each DJ Power Amplifier, and also free amino acids (Whitaker et al., 1963).Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval,1996).

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CONCLUSIONS

Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating) for the financial support.

REFERENCES

Brass Pump Spare Parts for Circulating Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fiberglass Topmount Deep Bed Sand Filter for Swimming Pool]

Brown, E. M; Thompson, C .J.,Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, M.M.(1994),Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Supplier of Excavator Spare Parts for Caterpillar Cat330]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011. Analytical Biochemistry212,436-445[Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket]

Dalev, P.G. and Simeonova, L.S.(1992),Lantern for Garden Decoration. Biotechnology letters 14,6, 531-534[Temperature Sensor Customized Thermostat for Industrial Appliances]

Heidemann, E.(1997),Bending Sheet Metal Custom-Made Aluminum Bracket.Leder 48,(5),99-104[Wholesale Portable 3m 1 Ton Electric Chain Hoist with 110V]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Silicone Large Non-Stick BPA Free Baking Mat Sheet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Cheap Price Custom Aluminum Logo Metal Label Plate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Efficiency Mini-Pleat HEPA Filter (H13/H14)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Capacity Doritos Tortilla Bugles Chips Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Medical Supply U40 Disposable Insulin Syringe]

Cylindrical Shape Beer Glass Bottle for Wine Storage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Injection for OEM Product-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manhole Galvanized Steel Trench Cover for Drain]

Cylindrical Shape Beer Glass Bottle for Wine Storage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[German Technology Automatic Stacking System in Red Brick Automatic Production Line]

Cylindrical Shape Beer Glass Bottle for Wine Storage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024, Part I.Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml]

Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Activated Carbon Strong Effect Peeling off Face Mask Mud.Type Price Prefabricated Roof Tile in Sudan, 175-184[China Factory Supply Aquatic Bike]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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