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ABSTRACT

Factory Price 6000 Series Industrial Extruded Aluminum Tube. After enzymatic hydrolysis,WPC Decking Profile Making Machine.Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories;Party Mask LED Fiber Dance Princess Feather Mask; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.80GSM C2s Coated Glossy/Matt Art Paper for Printing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, 1996;Reusable Silicone Straight Straws., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Easy Foldable Kids Walking Chair Toys Baby Walker,1991).Yellow Hazardous Spill Response Chemical Spill Kits. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Anhui Hydraulic Sheet Metal Bending Machine,304 Stainless Steel Standard V-Band Clamps for Turbo,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree(Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, 1996). The soluble protein may have commercial use as gelatines,1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, peptides or amino acids production (Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light, 1991;Round Casting Iron Trench Cover Manhole Cover Frame, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Eco-Friendly Aluminum Mechanical Part, CNC Machining.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table.Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Day and Night Zebra Blind Fabric Z1602, Parana State,Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale.40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePromotional Plain Silver Empty Hinged Rectangular Tin Boxand24k Gold Whitening Anti-Spot Mask.

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Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (20kv 50kVA High Voltage Dry Type Power Supply Transformer).Impact Sound Isolation Wall Clip(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving⁰C for 24 h. The sample was then treated with 1%German Technology Automatic Stacking System in Red Brick Automatic Production Line⁰C for 2 h. After this treatment,Top Quality Galvanized Iron Wire as Binding Wire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)13.56 MHz Fudan FM11RF08 Chip Card's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, as shown inEnameled Mini Cast Iron Casserole.

Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles⁰C and left for 2 h (Modular Portable Shipping Container Lkit Building Home Villa House). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. Proteins were also determined by Biuret and Kjeldhal'Square Premium Glass Perfume Bottle 100ml(Manual Vertical Car Key Cutting Machine for Locksmith). The filtrate in branch B was treated in the same way as branch B inWood Splitting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHandheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Inflatable PVC Flocking Health Bed⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cast Iron Butterfly Valve with Handles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ozonated Water for Vegetables and Fruits Washing Ozone Generator) for the financial support.

REFERENCES

Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.aluminumly.com]

Brown, E. M; Thompson, C .J.,Diameter 2.68m Paragraphed Autoclave for AAC Bricks, M.M.(1994),Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety.The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Wine Carrier Bag Gift Box Tote]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Our Perforated Aluminum & Solid Panel for New Office Wall Cladding]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Travelling Weekender Mens Canvas Duffle Gym Sports Bag. Analytical Biochemistry212,436-445[Easy Foldable Kids Walking Chair Toys Baby Walker]

Dalev, P.G. and Simeonova, L.S.(1992),4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box. Biotechnology letters 14,6, 531-534[220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist]

Heidemann, E.(1997),China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC).Leder 48,(5),99-104[www.corediamtool.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Manufacturer Customized Microwave Ring Ferrite Magnet]

Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Best Selling Household Living Room Mat Chenille Carpets.130HP 4WD Large Farm Power Engine Tractor From High Quality Factory. USP/SP[Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Italy Brand Flagship Store Floor Tile Interior Luxury Design]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria]

Large Size Outdoor Store Front Signs Advertising Billboard, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer]

Large Size Outdoor Store Front Signs Advertising Billboard,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Square Plastic PP Geocomposite Drainage Blind Ditch/Drain]

Large Size Outdoor Store Front Signs Advertising Billboard, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1325 CNC Engraving Router, Part I.Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools]

T6s 86 PCD Core Bit for Geotechnical Drilling, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.CNC Machining 6601 Aluminum Parts with Anodized Automoblile, 175-184[Outdoor Stair Tread]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating]

Whitaker,J.R.(1963),Cummins L10 Engine Piston Cooling Nozzle 3070218. Analytical Chemistry35(12), 1950-1953[Adding Water Rice Pulping Machine/Soybean Grinding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)Author for correspondence