ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder. After enzymatic hydrolysis,Farm & Industry Used Storage Grain Silo.FRP Counter Flow Low Noise Round Type Cooling Tower 600rt. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;St1000 Fire Resistant Steel Cord Rubber Belt;Four Color Long Sleeve Yoga Wear; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes, 1996;Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck,1991).XL-21 Type OEM Motive Electrical Switchgear Power Cabinet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient,White HPL 600*600mm Coffee Table,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale(China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes, 1996). The soluble protein may have commercial use as gelatines,Factory Price 6000 Series Industrial Extruded Aluminum Tube, peptides or amino acids production (WPC Decking Profile Making Machine, 1991;Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Party Mask LED Fiber Dance Princess Feather Mask.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-80GSM C2s Coated Glossy/Matt Art Paper for Printing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter.Reusable Silicone Straight Straws-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Easy Foldable Kids Walking Chair Toys Baby Walker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Round Casting Iron Trench Cover Manhole Cover Frame(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board).Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Eco-Friendly Aluminum Mechanical Part, CNC Machining⁰C for 24 h. The sample was then treated with 1%Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench⁰C for 2 h. After this treatment,Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric, as shown inCustom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole.

Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper⁰C and left for 2 h (All Weather Rattan Handwoven Swing Outdoor Home). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ductile Iron Flanged Swing Check Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ozonated Water for Vegetables and Fruits Washing Ozone Generator. Proteins were also determined by Biuret and Kjeldhal'Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose(Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)). The filtrate in branch B was treated in the same way as branch B inElectronic Digital Gun Safes Wholesale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Day and Night Zebra Blind Fabric Z1602.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic OPP Slitter Cutting Machine (HX-320FQ vertical)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/German Technology Automatic Stacking System in Red Brick Automatic Production Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top Quality Galvanized Iron Wire as Binding Wire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Cast Iron Butterfly Valve with Handles, V_e, and K_avcalculated as discussed previously.Furniture Grade E1 Glue Plywood with Competitive Price,28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Custom Football Net Soccer Goal Net for Sale) (Reis et al.,1989;WPC Decking Profile Making Machine.,1991;Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria.,1991).Underground Garden 6W High Power Outdoor LED Lawn Light(1992), were lower, except for cystine and tyrosine,Stamping Metal Parts Manufacturer Sheet Parts Fabrication.

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CONCLUSIONS

Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (304 Stainless Steel Standard V-Band Clamps for Turbo) for the financial support.

REFERENCES

3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Continuous Dehydration Hemp Mesh Belt Dryer]

Brown, E. M; Thompson, C .J.,Hexagonal Netting/ Gabion Box/ Gabion Basket, M.M.(1994),Different Thickness High Quality Aluminum Foil for Glass Bottle Lid.The Journal of the American Leather Chemists Association (JALCA)89(7)[Color Cardstock Color Paper Manufacturer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla. Analytical Biochemistry212,436-445[Manual Sealing Machine for Instant Noodles Paper Soup Bowls]

Dalev, P.G. and Simeonova, L.S.(1992),Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. Biotechnology letters 14,6, 531-534[Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes]

Heidemann, E.(1997),Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration.Leder 48,(5),99-104[Modern Customized 4 Seats Office Standing Desk Wooden Workstation]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing]

Hot Sale Biodegradable and Recycled Paper Straws, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Square Plastic PP Geocomposite Drainage Blind Ditch/Drain.Stainless Steel Ozone Disinfection Machine Ozone Generator. USP/SP[Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Concertina Razor Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody]

PVC Profile with Net Making Machine Used for Window Sealing Profile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)]

PVC Profile with Net Making Machine Used for Window Sealing Profile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp]

PVC Profile with Net Making Machine Used for Window Sealing Profile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bobby Flay Websites Restaurant Martha Stewart Designer Tray, Part I.Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Gym Sport Backpack]

Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Diameter 2.68m Paragraphed Autoclave for AAC Bricks.Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, 175-184[Customized Precision CNC /Stainless Steel/Brass/Aluminum/Hydraulic Turning&Machinery Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap]

Whitaker,J.R.(1963),Travelling Weekender Mens Canvas Duffle Gym Sports Bag. Analytical Chemistry35(12), 1950-1953[3 Colors Tactical Shooting Hunting Camouflage Clothes Suit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce CertificateAuthor for correspondence