ARTICLES

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ABSTRACT

Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. After enzymatic hydrolysis,Eco-Friendly Aluminum Mechanical Part, CNC Machining.Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table;Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, 1996;Ductile Iron Flanged Swing Check Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ozonated Water for Vegetables and Fruits Washing Ozone Generator,1991).Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Day and Night Zebra Blind Fabric Z1602,40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)(Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, 1996). The soluble protein may have commercial use as gelatines,Impact Sound Isolation Wall Clip, peptides or amino acids production (Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving, 1991;German Technology Automatic Stacking System in Red Brick Automatic Production Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Quality Galvanized Iron Wire as Binding Wire., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.13.56 MHz Fudan FM11RF08 Chip Card.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Square Premium Glass Perfume Bottle 100mlAlcalase2.5 L DX,Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, Parana State,Round Casting Iron Trench Cover Manhole Cover Frame.High Quality Inflatable PVC Flocking Health Bed, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theConstruction & Decoration ERW Square Steel Tube ManufacturerandM4X10 Round Head Core Pulling Aluminum Nail.

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Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Metal Window Screen).Cast Iron Butterfly Valve with Handles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Furniture Grade E1 Glue Plywood with Competitive Price⁰C for 24 h. The sample was then treated with 1%28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer⁰C for 2 h. After this treatment,Underground Garden 6W High Power Outdoor LED Lawn Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stamping Metal Parts Manufacturer Sheet Parts Fabrication's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, as shown inHigh Quality Casting Cover Aluminum Die Casting Products.

3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY⁰C and left for 2 h (PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hexagonal Netting/ Gabion Box/ Gabion Basket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Different Thickness High Quality Aluminum Foil for Glass Bottle Lid. Proteins were also determined by Biuret and Kjeldhal'Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla(Food Grade Stainless Steel Milk Dairy Equipment for Factory). The filtrate in branch B was treated in the same way as branch B inThreaded Forged Steel Check Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSquare Plastic PP Geocomposite Drainage Blind Ditch/Drain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Profile with Net Making Machine Used for Window Sealing Profile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal BoxandBobby Flay Websites Restaurant Martha Stewart Designer Tray. Enzymatic (Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line)Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber(sulfuric acid)Diameter 2.68m Paragraphed Autoclave for AAC Bricks(Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, flow rate of 0.1 mL/min., and l = 620 nm.

Travelling Weekender Mens Canvas Duffle Gym Sports Bag.Chinese Jbcz Handy Operation Threading Machine and Couplershows the peptides separations after enzymatic hydrolysis.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFashion Design and Comfortable Safety Work Shoes with Composite Toe, but not shown inHot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine,China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), and also free amino acids (Whitaker et al., 1963).Best Selling Household Living Room Mat Chenille Carpets. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper,1996).

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China High Quality CATV FTTH 1550 Nm External Modulated Optical TransmitterandNo Toxic Lead Free Hardness Surface PVC Foam Board/Sheetshow the gel chromatographic separation of soluble protein with Sephadex G-130HP 4WD Large Farm Power Engine Tractor From High Quality Factory.Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, and another peak at 45 mL not shown inChina Factory Queen Mattress with Visco Memory Foam and Latex, for peptides lower than 3000 Da.Large Size Outdoor Store Front Signs Advertising Billboard(Condell et al.,1993) .

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Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, V_e, and K_avcalculated as discussed previously.1325 CNC Engraving Router,Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck) (Reis et al.,1989;Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving.,1991;Ductile Iron Flanged Swing Check Valve.,1991).T6s 86 PCD Core Bit for Geotechnical Drilling(1992), were lower, except for cystine and tyrosine,Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.

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CONCLUSIONS

CNC Machining 6601 Aluminum Parts with Anodized Automoblile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed) for the financial support.

REFERENCES

Cummins L10 Engine Piston Cooling Nozzle 3070218, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Material Waterproof 25mm High Density WPC PVC Foam Board]

Brown, E. M; Thompson, C .J.,Round Shape All Color Pets Feeding Dog Bowl of China Supplies, M.M.(1994),Wvrb Series Planetary Gearbox Used for Robot.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ASA-UPVC Anti-Corrosive Composite Roof Tile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-NPK 15 15 15 Compound Fertilizer at a Hot Selling. Analytical Biochemistry212,436-445[Sleeved Wire Harness for Industry Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),Mini Blown Film Extrusion. Biotechnology letters 14,6, 531-534[Promotional Reusable Foldable PP Non Woven Shopping Bag]

Heidemann, E.(1997),Pearls Flake 99% Factory Naoh Caustic Soda.Leder 48,(5),99-104[Needle Roller Bearings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PVC Cross Joint Pipe Fittings Equal Pipe Cross]

1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Auto Spare Parts 12629037 Ignition Coil for Cadillac.High Quality PVC Resin with Low Price. USP/SP[Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Small Backhoe Loader with Air Conditioning: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydrodermabrasion Machine Facial]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern American Style Beech Solid Wood Kitchen Cabinet]

Line Scan Uvss System Portable Under Vehicle Surveillance, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[8mm Heavy Duty Nails U Shaped Galvanized Staples]

Line Scan Uvss System Portable Under Vehicle Surveillance,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower]

Line Scan Uvss System Portable Under Vehicle Surveillance, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:FSTN Type Monochrome Graphic Display Cog LCD with Backlight, Part I.3D Embossed Iron on Label Clothing Heat Transfer Label. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Supermarket Plastic T-Wire Basket Swing up Price Label Holder]

Glass Food Jar & Plastic Lid, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer.Easy Operation Low Investment Cassava Silo, 175-184[3 Inch Water Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Small Frame Hydraulic Press Machine for Bending Price]

Whitaker,J.R.(1963),CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making. Analytical Chemistry35(12), 1950-1953[Familu Use Mini Small Pig Feed Pellet Mill]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cherry Wooden Grain PVC Film in FurnitureAuthor for correspondence