ARTICLES

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ABSTRACT

Round Shape All Color Pets Feeding Dog Bowl of China Supplies. After enzymatic hydrolysis,Wvrb Series Planetary Gearbox Used for Robot.NPK 15 15 15 Compound Fertilizer at a Hot Selling. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mini Blown Film Extrusion;Pearls Flake 99% Factory Naoh Caustic Soda; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Spare Parts 12629037 Ignition Coil for Cadillac, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality PVC Resin with Low Price, 1996;Small Backhoe Loader with Air Conditioning., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Line Scan Uvss System Portable Under Vehicle Surveillance,1991).Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,FSTN Type Monochrome Graphic Display Cog LCD with Backlight,3D Embossed Iron on Label Clothing Heat Transfer Label,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Glass Food Jar & Plastic Lid(High Quality PVC Resin with Low Price, 1996). The soluble protein may have commercial use as gelatines,0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer, peptides or amino acids production (Easy Operation Low Investment Cassava Silo, 1991;CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Building Material High Quality 316 Stainless Steel Grating.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality PVC Resin with Low Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Quality Shaper Machine Bc6085 Shapping Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5, Parana State,Cummins L10 Engine Piston Cooling Nozzle 3070218.Free Sample! AC Power Contactor for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality PVC Resin with Low Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFull Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power PlantandAir Cooled Scroll Type Water Chiller for Industrial Process.

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PA System Loudspeaker 100W Outdoor Column Speaker (2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured).Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Breathable Surgical Disposable 3 Ply Earloop Face Mask⁰C for 24 h. The sample was then treated with 1%Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9⁰C for 2 h. After this treatment,Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flower Design Custom Made Silk Scarf, as shown in1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer.

New Design Indoor Artificial Mini Rockery Fountains⁰C and left for 2 h (Rectangular Box Facial Tissue Paper Fk-110). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Medical Endoscope Disposable Bite Block with Bandage. Proteins were also determined by Biuret and Kjeldhal'Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels(1.75mm 3mm ABS PLA PC 3D Printer Filament Machine). The filtrate in branch B was treated in the same way as branch B in2017 Latest Design Galvanized High Strength Hexagon Head Screw - Stainless Steel thread rod - Jiangyu . The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ladder Type Cable Tray with Ce/ GOST/ TUV/UL. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av12V Electric Portable Outdoor Water Fruit Car Fridge with Ce(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Cheap Price Custom Aluminum Logo Metal Label Plate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3120/17/38 Boltable Crane Rail Clips, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3.7kw Air Compressor Parts Oil Free Scroll Compressor Headsand2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide. Enzymatic (China Supplier Rubber Injection Molding Machine with Ce)Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China(sulfuric acid)Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery(High Quality PVC Resin with Low Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free, flow rate of 0.1 mL/min., and l = 620 nm.

Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag.Flexible Board FPC PCB Camera Module Rigid Flex PCBshows the peptides separations after enzymatic hydrolysis.Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBaking Soda 99.8% Min Sodium Bicarbonate, but not shown inHigh Quality PVC Resin with Low Price,High Quality & High Speed Straight Line Wire Drawing Machine, and also free amino acids (Whitaker et al., 1963).High Speed 3000W Fiber Laser Cutter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality PVC Resin with Low Price,1996).

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CONCLUSIONS

Embossed Textured Wallpaper for Solvent Printing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts) for the financial support.

REFERENCES

Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[17oz 500ml Cola Shaped Bottle Insulated Double Wall Vacuum High-Luminance Water Bottle]

Brown, E. M; Thompson, C .J.,4-14 Manual Staple Gun with Staples for Hand Tools, M.M.(1994),500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3).The Journal of the American Leather Chemists Association (JALCA)89(7)[JIS S Type Cast Iron Water Filter Dn25]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Smallest Portable Waste Transformer Oil Purifier Machine Zy-6. Analytical Biochemistry212,436-445[High Strength Rigid PVC Plastic Foam Board for FRP]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles. Biotechnology letters 14,6, 531-534[LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker]

Heidemann, E.(1997),High Speed Automatic Packing Machine (GK-650 BA).Leder 48,(5),99-104[New Product 1080P HD 30 Fps Small Button Police Wearable Body Worn Camera]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ASME/ASTM Butt-Welding Stainless Steel Plate Flange]

Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),5000-5900MHz 4G Built-in Automotive Antenna.Glass Lined Reactor for Chemical Factory. USP/SP[Transport System China Manufacturer for Table Belt Conveyor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Metal Stamping Stainless Steel Parts with CNC Machining: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Water Treatment Anthracite]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lab Multi-Function High Speed Mixer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fcy30 Transport Mini Tipping Dumper]

Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Supermarket Plastic T-Wire Basket Swing up Price Label Holder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table]

Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve]

Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, Part I.Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems]

Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Degradable PLA Plastic Bottle.Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, 175-184[Curing Lamp Infrared]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine]

Whitaker,J.R.(1963),20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House. Analytical Chemistry35(12), 1950-1953[2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding MachineAuthor for correspondence