ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace

ABSTRACT

for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile. After enzymatic hydrolysis,100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree.School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle;Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (125kHz RFID Hotel Key Card with T5577 Chip, 1996;USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag,1991).Crop Fertilizer Agriculture Nh4cl Ammonium Chloride. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale High Pressure 80L Nitrogen Gas Cylinder,10mm Red Green Yellow Warning China Supplier Signal Lamp,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor(125kHz RFID Hotel Key Card with T5577 Chip, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Thermostat Electric Heating Element for Solar Water Heaters, peptides or amino acids production (Phillip Countersunk Head Screw Hot DIP Galvanized, 1991;Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heat Shrink Automatic Flow Wrapping Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (125kHz RFID Hotel Key Card with T5577 Chip, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ductile Iron Standard Brackets Pipe Hanger.Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10mm Red Green Yellow Warning China Supplier Signal Lamp).AVR Se350 Automatic Voltage Regulator for Marathon Alternator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage⁰C for 24 h. The sample was then treated with 1%Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147⁰C for 2 h. After this treatment,High Strength Rigid PVC Plastic Foam Board for FRP. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, as shown inDurable Steel Retainer Plastic Road Safety Trench Cover.

Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve⁰C and left for 2 h (AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps. Proteins were also determined by Biuret and Kjeldhal'New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels(Wear Resitant Rope End Cap Plastic Flange Cover). The filtrate in branch B was treated in the same way as branch B inm.wh-machine.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avADSS Newest Ultra Therapy Skin Tightening 3D Hifu(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Ceramic Chrome Hybrid Bearing Oil, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CE Approved Manual Heat Press MachineandCAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder. Enzymatic (Digger Arm Small Wheel Loader (HQ908) with CE)15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer(sulfuric acid)Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output(125kHz RFID Hotel Key Card with T5577 Chip, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Water Based Emulsion Paint Production Line, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Water Based Emulsion Paint Production Line, flow rate of 0.1 mL/min., and l = 620 nm.

Cnm Single Strand Anchor Coupler.Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Signshows the peptides separations after enzymatic hydrolysis.Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water, but not shown inHigh Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles,18X16mesh Fiberglass Window Screen Insect Screen, and also free amino acids (Whitaker et al., 1963).Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (125kHz RFID Hotel Key Card with T5577 Chip,1996).

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2019 New Advertising Inflatables Air Walking Backpack Ball, V_e, and K_avcalculated as discussed previously.Side Pack Manual Gas Engine Power Brush Cutter,Punching Bending Welding Steel Work Fabrication. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Replacement Cell Phone LCD for iPhone 6s Spare Parts) (Reis et al.,1989;Phillip Countersunk Head Screw Hot DIP Galvanized.,1991;USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug.,1991).Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus(1992), were lower, except for cystine and tyrosine,Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears.

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CONCLUSIONS

1 Ton Chain Pulley Block with Geared Trolley. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Different Size Glass Test Tubes, High Quality Laboratory Test Tubes) for the financial support.

REFERENCES

Powder Coating Polyester Resin with Factory Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rolling Shutter Built-in Tubular Motor WiFi Smart Controller Yet846WiFi]

Brown, E. M; Thompson, C .J.,Auto Plastic Part Hardware Plastic Injection Mold, M.M.(1994),Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Diameter 2.68m Paragraphed Autoclave for AAC Bricks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-IEC 62196-2 Type 2 EV Charging 32A Male Plug. Analytical Biochemistry212,436-445[Ferrite Core for Power Transformer (Ee16e)]

Dalev, P.G. and Simeonova, L.S.(1992),PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter. Biotechnology letters 14,6, 531-534[PS 200 Upper Discharge Centrifuge]

Heidemann, E.(1997),180W 1850mm*80mm Reci Laser Tube.Leder 48,(5),99-104[Ce DOT Kgs Certified Low Pressure 18bar 13.4L Helium Gas Helium Tank for Balloon Filling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture]

Pond Self Priming Booster suction  Water Pump with Pressure Switch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM Factory Balloon Padding Textile Machinery.NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy. USP/SP[Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PE Biodegradable Sheet Product Line/Single Screw Extruder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Solar Panel System off-Grid PV Solar Systems 5kw for Home Power Energy System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wheel-Type-Carriage Precision Heavy Duty Linear Guide]

Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Concert Stage Equipment Aluminum Stage Portable Stage-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper]

Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ductile Iron Flanged Swing Check Valve]

Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home, Part I.22*30mm 12SMD 5050 LED Reading Light for Car. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3.5-7.0g Ozone Generator Purification ---Commercial]

SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness.Outdoor Cheap Portable Stage Aluminum Truss Stage, 175-184[Toilet Shower Beach Changing Room Tent High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[20L Water Tank Lid Mold 3D Printing Samples Available]

Whitaker,J.R.(1963),One Arm Motorized Multifunction Hospital Equipment for China Supply. Analytical Chemistry35(12), 1950-1953[ for Patio Garden Backyard (Warm white)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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