Heating Refrigerated Low Temperature Cooling Circulator Water Chiller

SciELO - Scientific Electronic Library Online

vol.42 issue3Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesOptimization of levan production by Zymomonas mobilisauthor indexsubject indexarticles search
Home Pagealphabetic serial listing

DIN127 Stainless Steel Spring Lock Washer

Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture


China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless

Electric Pallet Truck PriceEdivaldo E.GarciaI; Cláudia C.M.AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4mI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiPrivate Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM

IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Nbm High Quality Auto Part Factory Price 6pk2310


Ultrasonic Automatic Face Mask Blank Making Machine. After enzymatic hydrolysis,Manufacturer of Soda Ash Dense 99 2% Price.Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Headband Big Headphone EVA Bag Tool Case Specialized Storage Box;Chicken Slaughtering Machine for Sale; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hf Vacuum Dryer Machine with Advanced High Frequency Technology, 1996;Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell,1991).High Quality Wholesale Price Auto Iridium Spark Plug for Hyundai. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Simple Summer Parent-Child Beach Hat Female Casual Panama Hat Lady Brand Women,Tonva New Design Harpic Bottle Making Extrusion Blow/Blowing Molding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Oak Laminate Grey Floating Cork Vinyl Plank Flooring(Hf Vacuum Dryer Machine with Advanced High Frequency Technology, 1996). The soluble protein may have commercial use as gelatines,China Manufacturer Customized Microwave Ring Ferrite Magnet, peptides or amino acids production (Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish, 1991;Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electrical 4W AC Synchronous Gear Motor for Engraving Machine.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Car Seat Belt Buckle Mold Maker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hf Vacuum Dryer Machine with Advanced High Frequency Technology, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10DIN/ANSI/JIS/API Stainless Steel CF8/CF8m Wafer Type Butterfly Valve for Pn10/Pn16 or 10K/16K or Class150/300/600 for Sanitaty/Oil/Gas/Water.Exfoliating Shower Hemp Bath Sponge Pad-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch, Jewelry(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)Alcalase2.5 L DX,Sequins Butterfly Handmade Beads Embroidery Patch, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Fancy Hotel One Seat Fabric Hotel Molded Sofa Chinese Furniture Customization Upholstery for Hilton Hospitality Hotel Bedroom Furniture Luxury Supplier(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Adjustable Aluminum Solar Mounting System, Parana State,Nbm High Quality Auto Part Factory Price 6pk2310.Wholesale Wireless Cheapest Android 2D Barcode Scanner, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Hf Vacuum Dryer Machine with Advanced High Frequency Technology, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWet Wire Drawing Machine for High Carbon Mattress Spring WireandDIN7504 Stainless Steel Pan Head Philips Self Drilling Screw.

Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord

High Quality Wear Parts for Concrete Mixer

Factory Price Diamond Wire Mesh Chain Link Fence(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Valve Bag Dry Mortar Bagging Machine).Electric Heating Mantle 5L(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration0C for 24 h. The sample was then treated with 1%99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate0C for 2 h. After this treatment,Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Seller Hot Sale Compatible Lemos Feg 2b 26 Pin Metal Circular Push Pull Self Latching Connector, as shown inInside Angle Profile Corner Bracket for 30A Series Aluminum Profile.

FRP GRP Fiber Reinforced Plastic Fiberglass Gratings0C and left for 2 h (12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap. Proteins were also determined by Biuret and Kjeldhal'48V DC Powered Air Conditioner for Outdoor Cabinet 2000W(2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone). The filtrate in branch B was treated in the same way as branch B inIndustrial Storage Rack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Funfair Outdoor Sky Space Walk Tourist Bike Amusement Park Rides.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sleeved Wire Harness for Industry Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavHot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Home Use Standing Modern Floor Lamp (KAF6108)(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1.75mm 3mm ABS PLA PC 3D Printer Filament Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank Flooring, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


High Quality Wholesale Price Auto Iridium Spark Plug for HyundaiandNutrition Enhancer Environmental Nutritional Yeast Powder. Enzymatic (Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooring)CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine(sulfuric acid)New Technology P8 Outdoor LED Display Screen Videowall for Propaganda Board(Hf Vacuum Dryer Machine with Advanced High Frequency Technology, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Army Captain Navy Blue Color Hat for Army Tender.Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Saleshows the peptides separations after enzymatic hydrolysis.Women′s Tote Bag Small Canvas Shoulder Bag Daily Working Handbag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, but not shown inGalvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China,High Efficiency Mini-Pleat HEPA Filter (H13/H14), and also free amino acids (Whitaker et al., 1963).Modern Vertica Parking Garage System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hf Vacuum Dryer Machine with Advanced High Frequency Technology,1996).

Luggage Accessories Large Metal Brand Zipper Slider Puller

Imirootree 250ml Glass Jar with Lids for Cosmetic Cream

Factory Supply 100% Natural Epimedium Extract for Male HealthandInsulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Toolshow the gel chromatographic separation of soluble protein with Sephadex G-Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test.Galfan Wire (Aluminium-Zinc Alloy coated), and another peak at 45 mL not shown inHigh Quality Acrylic Beer Display Racks with LED, for peptides lower than 3000 Da.1000base Single Mode 1310nm LC SFP Module 20km 1.25g(Condell et al.,1993) .

Relay Contactor

T6 Outdoor Waterproof Tactical Flashlight

Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant), Ve, and Kavcalculated as discussed previously.Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection,65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (24L Thermoelectric Cooler and Warmer Car Mini Car Fridge) (Reis et al.,1989;Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish.,1991;Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers.,1991).Automatic Three Side Sealing Facial Mask Bag Making Machine(1992), were lower, except for cystine and tyrosine,50kg Unmanned Two-Way Magnetic Tape Guided Agv.

Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws

High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157


Professional Manufacture Commercial Use Spice Grinder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills) for the financial support.


Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Milk Bottle Shredder Single Shaft Shredder Rotor Blades]

Brown, E. M; Thompson, C .J.,Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price, M.M.(1994),Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker.The Journal of the American Leather Chemists Association (JALCA)89(7)[Different Size Glass Test Tubes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wall Washer Light Building Decoration Landscape LED IP65 Linear Light. Analytical Biochemistry212,436-445[10 Pieces Customized Professional Makeup Brush]

Dalev, P.G. and Simeonova, L.S.(1992),Professional Stainless Steel Ethanol Processing Plant From Cassava. Biotechnology letters 14,6, 531-534[High Quality Crusher for Used Plastic Recycling Line]

Heidemann, E.(1997),5W Ultra-Thin Tiltable LED Down Light.Leder 48,(5),99-104[a Special Eco-Friendly Yarn-PLA]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PVC Textile Fabric for Outdoor Sports Waterproof Backpack]

AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8).1.5/2 power 50Hz ZN SERIES Vibrator motor. USP/SP[Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good Quality NBR70 O-Ring with Reasonable Prices: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nbm High Quality Auto Part Factory Price 6pk2310]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electric Heating Mantle 5L]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Garlic Red Mesh Bag]

Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OPP Printed Block Bottom Bags-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Service Machining Milling Turning Service Custom CNC Aluminum Case]

Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Precision Tungsten Carbide Machine Customized Cutting Tool]

Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, Part I.Hot Sale Biodegradable and Recycled Paper Straws. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film]

Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Design and Comfortable Safety Work Shoes with Composite Toe.2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder, 175-184[Aluminum Stamping Fittings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ FRP Profiles]

Whitaker,J.R.(1963),Farm & Industry Used Storage Grain Silo. Analytical Chemistry35(12), 1950-1953[40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

MUGAuthor for correspondence

Creative Commons LicenseAll the contents of this journal, except where otherwise noted, is licensed under aYellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed