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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vertical Type Automatic Box Erector Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Air Cooled Scroll Type Water Chiller for Industrial Process, 1996;Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090,1991).Rotary Vibrating Sieve Machine for Compost. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud,Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa(Air Cooled Scroll Type Water Chiller for Industrial Process, 1996). The soluble protein may have commercial use as gelatines,Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, peptides or amino acids production (Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, 1991;Condoms/Sex Toys/Capsule Toy Vending Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Type 40kgs Movable Ice Maker for Store Use. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Air Cooled Scroll Type Water Chiller for Industrial Process, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts.Electric 100W Three Wheels Toy Car for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2*2 4G Lte MIMO Antenna Rrouter/CPE(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plain Makeup Bag Canvas Cosmetic Bag).China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cute Clear Fanny Pack Stadium Security PVC Waist Bag⁰C for 24 h. The sample was then treated with 1%Personalized Coffee Mug Cup with Custom Logo⁰C for 2 h. After this treatment,100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)10 Pieces Customized Professional Makeup Brush's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, as shown in7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane.

Electrophoresis Assembly Infilltration Tank for Effluent Treatment⁰C and left for 2 h (2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.700mm RP HP UHP High Quality Graphite Electrode. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Prefab Building Insulated Interior Concrete Partition Walls. Proteins were also determined by Biuret and Kjeldhal'Disk Filter System with Automatic Backwash Valves(Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)). The filtrate in branch B was treated in the same way as branch B inNylon Waterproof Pet Cushion (WY1204020-2A/C). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Eco-Friendly Recycling PS Foam Container Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCommercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Jacquard Football Fans Acrylic Sports Warmer Scarf⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Small Blinking Battery Operated Luminary Plastic LED Tea Lights, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mineral Industry Magnet Ore Ball Milling Machine.High Quality Orthopedic Fiberglass Splintshows the peptides separations after enzymatic hydrolysis.DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlain Chipboard, but not shown inCNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories,Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel, and also free amino acids (Whitaker et al., 1963).Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Air Cooled Scroll Type Water Chiller for Industrial Process,1996).

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Chemical Plant Transfer Industrial Water Pump, V_e, and K_avcalculated as discussed previously.Insecticidal Lamp with Solar Energy Frequent Vibration,Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Plastic Food Box Container Covers by Injection Mould Mold Tool) (Reis et al.,1989;Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube.,1991;Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China.,1991).Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics(1992), were lower, except for cystine and tyrosine,Galvanized Square Washer with Timber Constructions, DIN436DIN436.

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CONCLUSIONS

Do Not Enter Tape Warning Tape Caution Tape Danger Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging) for the financial support.

REFERENCES

CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Travel Toiletries Bag Foldable Children Travel Trolley Luggage Bag]

Brown, E. M; Thompson, C .J.,Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, M.M.(1994),Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist.The Journal of the American Leather Chemists Association (JALCA)89(7)[Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[25kg 50kg Plastic PP Woven Feed Bags]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Quality PVC Plastic Ceiling for Wall Panel. Analytical Biochemistry212,436-445[360 Degree Rotation Medical Seat Cushion]

Dalev, P.G. and Simeonova, L.S.(1992),Heavy Steel Structures for Power, Cement, Coal Plant. Biotechnology letters 14,6, 531-534[New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings]

Heidemann, E.(1997),Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+.Leder 48,(5),99-104[A4 X 4 Pockest Acrylic Literature Holder Brochure Holder Stand Display Stand]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit]

Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CE Approved Manual Heat Press Machine.T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820). USP/SP[Oak Laminate Grey Floating Cork Vinyl Plank Flooring]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Insulation Copper Wire Braiding Soft Electrical Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Headband Big Headphone EVA Bag Tool Case Specialized Storage Box]

Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System]

Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Home Use Standing Modern Floor Lamp (KAF6108)]

Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain, Part I.China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts]

AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner.Nbm High Quality Auto Part Factory Price 6pk2310, 175-184[Video Door Intercom System]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ 230V]

Whitaker,J.R.(1963),Ultrasonic Automatic Face Mask Blank Making Machine. Analytical Chemistry35(12), 1950-1953[160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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