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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. After enzymatic hydrolysis,Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator.Injector Pump Calibration Test Bench. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ASME/ASTM Butt-Welding Stainless Steel Plate Flange;500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Polyester / Polypropylene Woven Geotextile for Road Construction, 1996;Granite Stone Jaw Crusher of Mining Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor,1991).Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine,Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale All Kinds Cheap Plush Animal Baby Slippers(Polyester / Polypropylene Woven Geotextile for Road Construction, 1996). The soluble protein may have commercial use as gelatines,Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car, peptides or amino acids production (Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, 1991;Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Taiding Custom Tank Sheet Metal Fabrication. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Polyester / Polypropylene Woven Geotextile for Road Construction, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Airtight Candy Bin with Scoop.Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Familu Use Mini Small Pig Feed Pellet Mill, Parana State,Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable.3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Polyester / Polypropylene Woven Geotextile for Road Construction, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEnergy Saving Street Light E27 E40 27W LED Corn BulbandMulti-Function Small Tree Digging Shovel/Tree Machine.

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Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (1 Ton Chain Pulley Block with Geared Trolley).Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hairdressing Equipment Takara Belmont Barber Chair High Quality0C for 24 h. The sample was then treated with 1%Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)0C for 2 h. After this treatment,Buy Single Item Moving Bee Eye Light Lowly Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Customized Aluminum Foil Shisha Sheet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank, as shown inHigh Quality Spooling Pet Polyester Mylar Film for Cable Material.

Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment0C and left for 2 h (Micro-Combustion Tube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Rectangular Box Facial Tissue Paper Fk-110. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. Proteins were also determined by Biuret and Kjeldhal'Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door(Chemical Product Sodium Gluconate Used for Concrete Admixture). The filtrate in branch B was treated in the same way as branch B inGood Quality Boron Nitride Price for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,AAC Block Production Line / AAC Equipment Manufacturing/ Machinery.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavQ32 Series Tumble Belt/Crawler Shot Blasting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Galvanized Bar Grating(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Custom Self Adhesive Leather Patch for Jackets0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/380mm Working Area Concrete Diamond Floor Edge Grinder, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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Custom Sheet Metal Stamping Bending Product Manufacturing Service.High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unitshows the peptides separations after enzymatic hydrolysis.Waterproof EVA Rubber Roofing Material for Shingle Building(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Manufacturer Industrial Aluminum Floor Bar Grating, but not shown inStainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain,OEM Gray Cast Iron Sand Casting Products with Universal Parts, and also free amino acids (Whitaker et al., 1963).U. S. Type Drop Forged Wire Rope Clip. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Polyester / Polypropylene Woven Geotextile for Road Construction,1996).

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Aluminum Low Profile Adjustable Bracket, Ve, and Kavcalculated as discussed previously.Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter,High Quality Acrylic Beer Display Racks with LED. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Smallest Portable Waste Transformer Oil Purifier Machine Zy-6) (Reis et al.,1989;Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane.,1991;Granite Stone Jaw Crusher of Mining Machine.,1991).Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)(1992), were lower, except for cystine and tyrosine,Baby Feeding Chair Plastic Chair for Feeding Hebei China.

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Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Export Grade Citric Acid Monohydrate as Detergent) for the financial support.


Plastic Injection Molding for Auto Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Li-ion Battery Power Tool Cordless Hammer Drill]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System. Analytical Biochemistry212,436-445[Wholesale 30W LED Down Lights with Budget Price]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Trendy High Quality 925 Stering Silver Tennis Glod Charm]

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Reis, M. and Beleza, V.(1989), Utilization of leather waste-Precision Engineering Parts Bearing Support Unit for Ball Screw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[6mm Aluminum Honeycomb Panel Composite Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Trailer RV Vision Security]

Whitaker,J.R.(1963),DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh. Analytical Chemistry35(12), 1950-1953[Custom Waterproof Pizza Sign Large Advertising Signboard]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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